Imprecise excision of the progenitor insertion, resulting in a deletion that removes sequences from +92 to +2416 relative to the MAN1 transcription start site. 240bp of the P{SUPor-P} element remain at the original insertion site.
Homozygous embryos (lacking zygotic MAN1 function) mostly show lethality during the pupal-to-adult transition.
Only 8% of homozygous embryos derived from homozygous females (lacking maternal and zygotic MAN1 function) hatch. A few of these larvae survive to adulthood, but die within a day of eclosion.
The morphology of salivary gland nuclei is normal in mutant third instar larvae.
Homozygous adults show wing patterning defects; the organisation of longitudinal veins and crossveins is disrupted. Often, the wings show thickening of all longitudinal veins, a variable number of anterior crossveins (1-4), branching of the posterior crossvein and folds in the wing blade. The wings are held-out in mutant adults, but the structure of the indirect flight muscles is not grossly altered.
Homozygous adults climb shorter distances than controls in a climbing assay. The defects become more pronounced in older flies.
Homozygous males have small, disorganised reproductive tissues that contain motile sperm.
Homozygous females have reduced fecundity.
3 day old homozygous females have ovarioles containing egg chambers representing all stages of oogenesis, with each containing the appropriate numbers of nurse cells and oocyte cells. The ovaries have an increased number of stage 8 egg chambers with condensed DNA in the nurse cell nuclei, indicative of apoptosis. Ovaries from 10 day old homozygous females lack early stage egg chambers, showing an accumulation of stage 14 oocytes. This may be indicative of a possible failure in egg deposition, and correlates with age-dependent changes in fecundity compared to controls.
MAN1Δ26/MAN1Δ81, OtePK/OteB279 has some die during P-stage phenotype
MAN1Δ26/MAN1Δ81, OtePK/OteB279 has some die during larval stage phenotype
MAN1Δ26/MAN1Δ81, bocksΔ10/bocksΔ66 has some die during P-stage phenotype
MAN1Δ26/MAN1Δ81, bocksΔ10/bocksΔ66 has some die during larval stage phenotype
