FlyBase Allele Report: Dmel\Myd88[kra-56]

General Information

Symbol

Dmel\Myd88^kra-56

Species

D. melanogaster

Name

FlyBase ID

FBal0144724

Feature type

allele

Associated gene

Dmel\Myd88

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Luschnig et al., 2004)

Mutagen

ethyl methanesulfonate

(Luschnig et al., 2004, Luschnig et al., 2004)

Progenitor genotype

Cytology

Description

Amino acid replacement: R298C. Nucleotide substitution: C?T.

(Charatsi et al., 2003)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:9,305,327..9,305,327 [-]

Nucleotide change:

C9305327T

Reported nucleotide change:

C?T

Amino acid change:

R298C | Myd88-PA; R298C | Myd88-PB; R298C | Myd88-PC

Reported amino acid change:

R298C

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Charatsi et al., 2003)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell number | embryonic stage

(Foldi et al., 2017)

female sterile | recessive

(Luschnig et al., 2004)

increased cell death | pupal stage (with Df(2R)BSC279)

(Foldi et al., 2017)

lethal | abnormal dorsal/ventral axis specification | maternal effect (with Myd88^kra-1)

(Charatsi et al., 2003)

lethal | abnormal dorsal/ventral axis specification | maternal effect | recessive

(Charatsi et al., 2003)

lethal - all die during embryonic stage | germline clone | maternal effect | recessive

(Luschnig et al., 2004)

lethal - all die during embryonic stage | germline clone | non-rescuable maternal effect | recessive

(Luschnig et al., 2004)

some die during embryonic stage | maternal effect | recessive

(Luschnig et al., 2004)

some die during embryonic stage | non-rescuable maternal effect | recessive

(Luschnig et al., 2004)

some die during pupal stage

(Foldi et al., 2017)

viable

(Luschnig et al., 2004, Luschnig et al., 2004)

Phenotype Manifest In

Detailed Description

Statement

Reference

Myd88^kra-56 mutants show only moderate loss of eve-positive neurons in the embryonic central nervous system (only significant for lateral and U/CQ eve[+] neurons) compared to controls.

Myd88^kra-56/Df(2R)BSC279 mutant pupae display a significant increase in the number of apoptotic (Dcp-1-positive) cells in pupal ventral nerve cord. Myd88^kra-56 homozygotes are semi-lethal with a lethality phase at pupariation, but the levels of cell death in the pupal ventral nerve cord are comparable to controls.

(Foldi et al., 2017)

Weak allele. Embryos derived from females carrying homozygous germ line clones are dorsalised.

(Luschnig et al., 2004)

Embryos derived from females carrying homozygous germ line clones are partially dorsalised.

(Luschnig et al., 2004)

Embryos laid by homozygous Myd88^kra-56 or Myd88^kra-56/Myd88^kra-1 mothers fail to gastrulate properly and die as hollow tubes of dorsal cuticle. The Filzkorper is present in these embryos.

(Charatsi et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Myd88^kra-56/Myd88^kra-56 is an enhancer of increased cell death | pupal stage phenotype of Scer\GAL4^elav.PU, Toll-6^{C1020Y.UAS.Tag:FLAG}

(Foldi et al., 2017)

Myd88^kra-56/Myd88^kra-56 is an enhancer of increased cell death | pupal stage phenotype of Sarm^EP3610, Scer\GAL4^elav.PU

(Foldi et al., 2017)

Phenotype Manifest In

Enhanced by

Statement

Reference

Myd88^kra-56 has embryonic/larval central nervous system | embryonic stage phenotype, enhanceable by Toll-6²⁶/Toll-6²⁶

(Foldi et al., 2017)

Myd88^kra-56 has neuron | embryonic stage phenotype, enhanceable by Toll-7^P8/Toll-7^P8/Toll-6²⁶/Toll-6²⁶

(Foldi et al., 2017)

NOT Enhanced by

Statement

Reference

Myd88^kra-56 has embryonic/larval central nervous system | embryonic stage phenotype, non-enhanceable by Toll-7^P8/Toll-7^P8

(Foldi et al., 2017)

Myd88^kra-56 has neuron | embryonic stage phenotype, non-enhanceable by Toll-7^P8/Toll-7^P8

(Foldi et al., 2017)

Myd88^kra-56 has embryonic/larval central nervous system | embryonic stage phenotype, non-enhanceable by Toll-6²⁶/Toll-6²⁶

(Foldi et al., 2017)

Myd88^kra-56 has neuron | embryonic stage phenotype, non-enhanceable by Toll-6²⁶/Toll-6²⁶

(Foldi et al., 2017)

Enhancer of

Statement

Reference

Myd88^kra-56/Myd88^kra-56 is an enhancer of larval ventral nerve cord | pupal stage phenotype of Scer\GAL4^elav.PU, Toll-6^{C1020Y.UAS.Tag:FLAG}

(Foldi et al., 2017)

Myd88^kra-56/Myd88^kra-56 is an enhancer of larval ventral nerve cord | pupal stage phenotype of Sarm^EP3610, Scer\GAL4^elav.PU

(Foldi et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The moderate loss of eve-positive neurons (from the lateral, EL, and U/CQ eve[+] clusters) in embryonic central nervous system characteristic for Myd88^kra-56 mutants is not exacerbated by combination with two copies of either Toll-7^P8 or Toll-6²⁶. However, in Myd88^kra-56 Toll-7^P8;Toll-6²⁶ triple mutant embryos the neuronal loss is enhanced further as in addition to loss of neurons from the EL+U/CQ clusters, the number of neurons in the aCC pCC and RP2 groups are also reduced compared to controls.

The increased cell death (number of apoptotic Dcp-1-positive cells) in the ventral nerve cord of pupae expressing either Toll-6^{C1020Y.Scer\UAS.T:Zzzz\FLAG} or Ect4^EP3610 under the control of Scer\GAL4^elav.PU can be increased further by Myd88^kra-56 homozygosity.

(Foldi et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

2R-56-12

(Luschnig et al., 2004)

MyD88^kra56

(Foldi et al., 2017)

Myd88^kra-56

kra⁵⁶

(Charatsi et al., 2003)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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