FlyBase Allele Report: Dmel\Sarm[EP3610]

General Information

Symbol

Dmel\Sarm^EP3610

Species

D. melanogaster

Name

FlyBase ID

FBal0337127

Feature type

allele

Associated gene

Dmel\Sarm

Associated Insertion(s)

P{EP}Sarm^EP3610

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Associated Insertion(s)

P{EP}Sarm^EP3610

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:8,063,844..8,063,844 [+]

(Waller and Collins, 2023, Foldi et al., 2017)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | larval stage, with Scer\GAL4^Toll-6-D42

(Waller and Collins, 2023)

decreased cell number | pupal stage, with Scer\GAL4^elav.PU

(Foldi et al., 2017)

increased cell death | pupal stage, with Scer\GAL4^elav.PU

(Foldi et al., 2017)

Phenotype Manifest In

embryonic/larval motor neuron | larval stage, with Scer\GAL4^Toll-6-D42

(Waller and Collins, 2023)

embryonic/larval neuromuscular junction | larval stage, with Scer\GAL4^Toll-6-D42

(Waller and Collins, 2023)

larval ventral nerve cord | pupal stage, with Scer\GAL4^elav.PU

(Foldi et al., 2017)

neuron | pupal stage, with Scer\GAL4^elav.PU

(Foldi et al., 2017)

Detailed Description

Statement

Reference

Expression of Ect4^EP3610 under the control of Scer\GAL4^elav.PU results in increased number of apoptotic (Dcp-1-positive) cells and loss of eve-positive neurons in pupal ventral nerve cord (VNC) as compared to driver-only controls. This reduction in the number of eve+ neurons in pupal VNC is also observed when expression of Ect4^EP3610 is driven by Scer\GAL4^Myd88-NP6394, which also leads to general decrease in cell numbers within the drivers expression domain in the VNC (assayed using a nuclear histone-reporter).

(Foldi et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Sarm^EP3610, Scer\GAL4^elav.PU has increased cell death | pupal stage phenotype, enhanceable by Myd88^kra-56/Myd88^kra-56

(Foldi et al., 2017)

Suppressed by

Statement

Reference

Sarm^EP3610, Scer\GAL4^Toll-6-D42 has abnormal neuroanatomy | larval stage phenotype, suppressible by kay^Fbz.UAS, Scer\GAL4^Toll-6-D42

(Waller and Collins, 2023)

Sarm^EP3610, Scer\GAL4^elav.PU has increased cell death | pupal stage phenotype, suppressible by bsk^GD10555, Scer\GAL4^elav.PU

(Foldi et al., 2017)

NOT suppressed by

Statement

Reference

Sarm^EP3610, Scer\GAL4^elav.PU has increased cell death | pupal stage phenotype, non-suppressible by wek^EX14/Df(2L)BSC690

(Foldi et al., 2017)

Suppressor of

Statement

Reference

Sarm^EP3610, Scer\GAL4^tey-5053A is a suppressor | partially of abnormal neuroanatomy | larval stage phenotype of Scer\GAL4^tey-5053A, raw^KK104042

(Waller and Collins, 2023)

Phenotype Manifest In

Enhanced by

Statement

Reference

Sarm^EP3610, Scer\GAL4^elav.PU has larval ventral nerve cord | pupal stage phenotype, enhanceable by Myd88^kra-56/Myd88^kra-56

(Foldi et al., 2017)

Suppressed by

Statement

Reference

Sarm^EP3610, Scer\GAL4^Toll-6-D42 has embryonic/larval neuromuscular junction | larval stage phenotype, suppressible by kay^Fbz.UAS, Scer\GAL4^Toll-6-D42

(Waller and Collins, 2023)

Sarm^EP3610, Scer\GAL4^Toll-6-D42 has embryonic/larval motor neuron | larval stage phenotype, suppressible by kay^Fbz.UAS, Scer\GAL4^Toll-6-D42

(Waller and Collins, 2023)

Sarm^EP3610, Scer\GAL4^elav.PU has larval ventral nerve cord | pupal stage phenotype, suppressible by bsk^GD10555, Scer\GAL4^elav.PU

(Foldi et al., 2017)

NOT suppressed by

Statement

Reference

Sarm^EP3610, Scer\GAL4^elav.PU has larval ventral nerve cord | pupal stage phenotype, non-suppressible by wek^EX14/Df(2L)BSC690

(Foldi et al., 2017)

Suppressor of

Statement

Reference

Sarm^EP3610, Scer\GAL4^tey-5053A is a suppressor | partially of distal axon | larval stage phenotype of Scer\GAL4^tey-5053A, raw^KK104042

(Waller and Collins, 2023)

Sarm^EP3610, Scer\GAL4^tey-5053A is a suppressor | partially of embryonic/larval motor neuron | larval stage phenotype of Scer\GAL4^tey-5053A, raw^KK104042

(Waller and Collins, 2023)

Additional Comments

Genetic Interactions

Statement

Reference

The increased cell death (number of apoptotic Dcp-1-positive cells) in the ventral nerve cord of pupae expressing Ect4^EP3610 under the control of Scer\GAL4^elav.PU can be increased further by Myd88^kra-56 homozygosity and is efficiently suppressed by co-expression of bsk^GD10555 but not by combination with wek^EX14/Df(2L)BSC690.

(Foldi et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

17144

w¹¹¹⁸; P{EP}Sarm^EP3610/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Ect4^EP3610

Sarm^EP3610

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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