FB2024_03 , released June 25, 2024
Allele: Dmel\wekEX14
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General Information
Symbol
Dmel\wekEX14
Species
D. melanogaster
Name
FlyBase ID
FBal0194093
Feature type
allele
Associated gene
Dmel\wek
Associated Insertion(s)
P{3'PZ}wekEX14
Carried in Construct
Key Links
Genomic Maps

Allele class

loss of function allele

Mutagen
Nature of the Allele
Allele class

loss of function allele

(Chen et al., 2006)
Mutagen
Delta2-3 transposase
(Chen et al., 2006)
Progenitor genotype
wek05271a
(Chen et al., 2006)
Associated Insertion(s)
P{3'PZ}wekEX14
Cytology
Description

Imprecise excision of the P{PZ} element. A 1.1kb fragment from the 3' end of the element is left behind (inserted in reverse orientation) at nucleotide 13 and nucleotides 6-13 are duplicated.

(Chen et al., 2006)
Mutations Mapped to the Genome
Curation Data
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      wekEX14/Df(2L)BSC690 pupae display reduced levels of cell death (number of Dcp-1-positive cells) in the ventral nerve cord relative to controls but the number of eve-positive neurons is unaffected.

      (Foldi et al., 2017)

      Embryos

      derived from mothers homozygous mutant for wekEX14 in the germline develop dorsalized cuticular structures with a range of phenotypes. 51% of these embryos show an extreme dorsalized phenotype as they secrete a hollow tube of dorsal epidermis, while the other 49% lack ventral and lateral structures but retain the dorsolaterally derived filzkorper.

      Although weklor/wekEX14 transheterozygotes are pupal lethal, some survive to adulthood but have crooked legs.

      Embryos derived from weklor/wekEX14 mothers exhibit a dorsalized phenotype that is weaker than embryos derived from wekEX14 mothers, but stronger than embryos derived from weklor mothers.

      (Chen et al., 2006)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressor of
      NOT Suppressor of
      Statement
      Reference

      wekEX14/Df(2L)BSC690 is a non-suppressor of increased cell death | pupal stage phenotype of SarmEP3610, Scer\GAL4elav.PU

      (Foldi et al., 2017)
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The increased cell death (number of apoptotic Dcp-1-positive cells) in the ventral nerve cord of pupae expressing Toll-6C1020Y.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4elav.PU can be rescued by combination with wekEX14/Df(2R)BSC279. In contrast, the increase in Dcp-1-positive cells in pupal VNC induced by Scer\GAL4elav.PU-driven expression of Ect4EP3610 cannot be suppressed by loss of combination with wekEX14/Df(2L)BSC690).

      (Foldi et al., 2017)

      The variable dorsalized embryonic phenotype of wekEX14 mutants is enhanced by mutations in the genes Tl, pll and tub. While 51% of wekEX14 embryos show an extreme dorsalized phenotype, this penetrance is increased to 97% in wekEX14; Tlr4/+ embryos, 92% in wekEX14; pll7/+ embryos and 96% in wekEX14; tub2/+ embryos.

      Embryos produced by weklor/wekEX14; Tl3/+ mothers show a biphasic phenotype distribution. 76% show a moderately dorsalized phenotype; these embryos lack ventral and lateral structures but retain the dorsolaterally-derived filzkorper. 24% show a lateralized phenotype; these embryos are elongated and thin in appearance and contain fine rings of laterally-derived ventral denticle belts.

      (Chen et al., 2006)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      wekEX14/Df(2L)BSC690 is rescued by Scer\GAL4elav.PU/wekUAS.ORF.GW.Tag:HA

      (Foldi et al., 2017)
      Comments

      The reduced level of cell death (decreased number of Dcp-1-positive cells) observed in the ventral nerve cord (VNC) of wekEX14/Df(2L)BSC690 and the increase in cell death induced by Scer\GAL4elav.PU-driven expression of wekScer\UAS.ORF.GW.T:Ivir\HA1 can both be mitigated by combining the two manipulations together: The number of apoptotic cells in the VNC of wekEX14/Df(2L)BSC690 mutants expressing wekScer\UAS.ORF.GW.T:Ivir\HA1 is intermediate to each of the two perturbations and higher than in controls.

      (Foldi et al., 2017)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Based on the extent of the dorsalized phenotype in embryos from germ-line clones, the following wek alleles can be ranked from strongest to weakest as follows: wekEX14 > wekRAR14 > weklor.

      (Chen et al., 2006)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      wekEX14
      (Foldi et al., 2017, Chen et al., 2006)
      Name Synonyms
      Secondary FlyBase IDs
        References (2)