FlyBase Allele Report: Dmel\Stat92E[397]

General Information

Symbol

Dmel\Stat92E³⁹⁷

Species

D. melanogaster

Name

FlyBase ID

FBal0130586

Feature type

allele

Associated gene

Dmel\Stat92E

Associated Insertion(s)

Carried in Construct

Also Known As

stat³⁹⁷

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Silver and Montell, 2001)

Progenitor genotype

Cytology

Description

Nucleotide substitution: G?A.

(Silver and Montell, 2001)

Amino acid replacement: W594term.

(Silver and Montell, 2001)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:20,539,772..20,539,772 [-]

Nucleotide change:

G20539772A

Reported nucleotide change:

G?A

Amino acid change:

Reported amino acid change:

W594term

Comment:

G to A nucleotide change at the second or third position of the Trp codon leads to a nonsense mutation (exact site of mutation unspecified).

(Silver and Montell, 2001)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates hematologic cancer

modeled by hop^T42

(Bausek and Zeidler, 2014)

modeled by hop^Tum

(Bailetti et al., 2019)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Stat92E³⁹⁷ mutant clones exhibit defects in enterocyte differentiation.

(Zhai et al., 2015)

The eyes of Stat92E³⁹⁷ mutant flies show a mild but significant reduction in eye size.

(Wang et al., 2014)

30% of Stat92E³⁹⁷/Df(3R)H-B79 animals die during embryogenesis and the remainder die during the first larval instar.

Mosaic animals in which the eye-antennal disc is almost completely homozygous for Stat92E³⁹⁷ show a reduced eye phenotype.

(Ekas et al., 2010)

Few escapers with eye-antennal disc Stat92E³⁹⁷ clones (in the Minute background) exhibit often only a rudimentary head when compared to controls.

(Flaherty et al., 2010)

Wild type eyes containing Stat92E³⁹⁷ mutant clones are normal in size. Eyes containing Stat92E³⁹⁷ mutant clones in a Minute background are small compared with Minute controls. Elimination of cells adjacent to Stat92E³⁹⁷ mutant clones (using hid) also results in reduced eye size.

(Wu et al., 2010)

Stat92E³⁹⁷/+ third instar larval lymph glands show hyperplasia and increased plasmatocyte differentiation. There is no marked increase in lamellocyte differentiation.

(Gao et al., 2009)

Stat92E³⁹⁷ wing disc clones average 6.4% of the wing pouch area, compared to 28% for wild-type clones. When wing discs are subjected to x-irradiation (5,000 rad daily for 3 days), Stat92E³⁹⁷ clones take up 1.1% of the total pouch area. The level of apoptosis observed is increased in mutant wing discs subjected to x-irradiation compared to controls.

(Betz et al., 2008)

Adults with Stat92E³⁹⁷ clones in a Minute background have aberrantly small eyes.

(Ekas et al., 2006)

In Stat92E^EP3391/Stat92E³⁹⁷ egg chambers, border cell migration is reduced but border cell number is not affected.

(Silver et al., 2005)

Homozygous mutant mosaic egg chambers display severe border cell migration defects. Border cells frequently fail to penetrate into the nurse cell clusters and can be found at the anterior tip or along the sides of the egg chamber. Alternatively the cells migrate partially, but fail top reach the oocyte by stage 10.

(Silver and Montell, 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Stat92E³⁹⁷ has increased cell death | conditional | third instar larval stage phenotype, suppressible by Scer\GAL4^Tub.PU/Diap1^{UAS.Tag:MYC.cBa}

(Betz et al., 2008)

Enhancer of

Statement

Reference

Stat92E³⁹⁷/Stat92E[+] is an enhancer of visible phenotype of upd3^x21b

(Wang et al., 2014)

Stat92E³⁹⁷/Stat92E[+] is an enhancer of visible phenotype of upd3^d232a

(Wang et al., 2014)

NOT Enhancer of

Statement

Reference

Stat92E³⁹⁷/Stat92E[+] is a non-enhancer of visible phenotype of upd3^x21b

(Wang et al., 2014)

Stat92E³⁹⁷/Stat92E[+] is a non-enhancer of visible phenotype of upd3^d232a

(Wang et al., 2014)

Suppressor of

Statement

Reference

Stat92E³⁹⁷ is a suppressor of increased size | third instar larval stage phenotype of bi^omb3-GAL4

(Tsai et al., 2015)

Stat92E³⁹⁷/Stat92E[+] is a suppressor of melanotic mass phenotype phenotype of hop^T42

(Bausek and Zeidler, 2014)

Phenotype Manifest In

Suppressed by

Statement

Reference

Stat92E³⁹⁷ has enterocyte | somatic clone phenotype, suppressible by Sox21a^UAS.Enh/Scer\GAL4^Tub.PU

(Zhai et al., 2015)

Stat92E³⁹⁷ has wing disc | conditional | third instar larval stage phenotype, suppressible by Scer\GAL4^Tub.PU/Diap1^{UAS.Tag:MYC.cBa}

(Betz et al., 2008)

Enhancer of

Statement

Reference

Stat92E³⁹⁷/Stat92E[+] is an enhancer of wing phenotype of upd3^d232a

(Wang et al., 2014)

Stat92E³⁹⁷/Stat92E[+] is an enhancer of haltere phenotype of upd3^d232a

(Wang et al., 2014)

NOT Enhancer of

Statement

Reference

Stat92E³⁹⁷/Stat92E[+] is a non-enhancer of eye phenotype of upd3^x21b

(Wang et al., 2014)

Stat92E³⁹⁷/Stat92E[+] is a non-enhancer of eye phenotype of upd3^d232a

(Wang et al., 2014)

Suppressor of

Statement

Reference

Stat92E³⁹⁷ is a suppressor of eye disc | third instar larval stage phenotype of bi^omb3-GAL4

(Tsai et al., 2015)

Stat92E³⁹⁷/Stat92E[+] is a suppressor of melanotic mass phenotype of hop^T42

(Bausek and Zeidler, 2014)

Other

Statement

Reference

Stat92E³⁹⁷/Stat92E[+], upd3^x21b has wing phenotype

(Wang et al., 2014)

Stat92E³⁹⁷/Stat92E[+], upd3^x21b has haltere phenotype

(Wang et al., 2014)

Ras85D^ΔC40B, Stat92E³⁹⁷ has intestinal stem cell phenotype

(Jiang et al., 2011)

Ras85D^ΔC40B, Stat92E³⁹⁷ has enterocyte phenotype

(Jiang et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Sox21a^Scer\UAS.Enh under the control of Scer\GAL4^tub.PU can partially suppress the defects in enterocyte differentiation seen in Stat92E³⁹⁷ mutant clones.

(Zhai et al., 2015)

One copy of Stat92E³⁹⁷ modestly but significantly enhances the reduction in eye size seen in upd3^d232a mutant flies, but the reduction in eye size is similar to that seen in Stat92E³⁹⁷ mutants alone.

One copy of Stat92E³⁹⁷ strongly enhances the outstretched wing and held down haltere phenotypes seen in upd3^d232a mutant flies.

Stat92E³⁹⁷ does not significantly enhances the reduction in eye size seen in upd3^x21b mutant flies.

Stat92E³⁹⁷ produces outstretched wing and held down haltere phenotypes in upd3^x21b mutant flies.

(Wang et al., 2014)

Ras85D^ΔC40B, Stat92E³⁹⁷ double mutant intestinal stem cell clones are smaller than adjacent cells and lack enterocyte cells.

(Jiang et al., 2011)

When th^Scer\UAS.cBa is over-expressed in the wing disc under the control of Scer\GAL4^tub.PU from first instar larval stage (by heat-shock induction to inactivate Scer\GAL80^αTub84B.PL), Stat92E³⁹⁷ mutant clones take up 7.7% of the wing pouch after x-irradiation treatment (5,000 rads daily for 3 days). Low levels of cell death are observed.

(Betz et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Stat92E^56D3

(Silver and Montell, 2001)

Stat92E^80A7

(Silver and Montell, 2001)

Stat92E^85C9

(Silver and Montell, 2001)

Stat92E⁰⁶³⁴⁶

(Silver and Montell, 2001)

Rescued by

Stat92E³⁹⁷/Stat92E⁰⁶³⁴⁶ is rescued by Stat92E^{sfGFP,Tag:FLAG}

(Sotillos et al., 2013)

Stat92E³⁹⁷ is rescued by Stat92E^UASp.Tag:HA/Scer\GAL4^ey.PH

(Ekas et al., 2010)

Stat92E³⁹⁷ is rescued by Stat92E^{ΔN.UASp.Tag:HA}/Scer\GAL4^ey.PH

(Ekas et al., 2010)

Stat92E³⁹⁷ is rescued by Stat92E^{ΔC.UASp.Tag:HA}/Scer\GAL4^ey.PH

(Ekas et al., 2010)

Not rescued by

Stat92E³⁹⁷ is not rescued by Stat92E^{R442P.UASp.Tag:HA}/Scer\GAL4^ey.PH

(Ekas et al., 2010)

Stat92E³⁹⁷ is not rescued by Stat92E^{Y711F.UASp.Tag:HA}/Scer\GAL4^ey.PH

(Ekas et al., 2010)

Comments

The mutant eye phenotype seen in mosaic animals in which the eye-antennal disc is almost completely homozygous for Stat92E³⁹⁷ is rescued by Stat92E^{Scer\UAS.P\T.T:Ivir\HA1}, Stat92E^{ΔN.Scer\UAS.P\T.T:Ivir\HA1} or Stat92E^{ΔC.Scer\UAS.P\T.T:Ivir\HA1} expressed under the control of Scer\GAL4^ey.PH.

The mutant eye phenotype seen in mosaic animals in which the eye-antennal disc is almost completely homozygous for Stat92E³⁹⁷ is not rescued by Stat92E^{Y711F.Scer\UAS.P\T.T:Ivir\HA1} or Stat92E^{R442P.Scer\UAS.P\T.T:Ivir\HA1} expressed under the control of Scer\GAL4^ey.PH.

(Ekas et al., 2010)

Stat92E³⁹⁷ fails to complement Stat92E^56D3, Stat92E^80A7 and Stat92E^85C9 with respect to lethality.

(Silver and Montell, 2001)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

STAT92E³⁹⁷

(Tsai et al., 2015)

Stat92E³⁹⁷

(Bailetti et al., 2019, Fregoso Lomas et al., 2016, Monahan and Starz-Gaiano, 2016, Wang et al., 2014, Woodfield et al., 2013, Ekas et al., 2010, Flaherty et al., 2010, Gutierrez-Aviño et al., 2009)

Stat³⁹⁷

(Zhai et al., 2015)

stat92E³⁹⁷

(Saadin and Starz-Gaiano, 2018, Bausek and Zeidler, 2014, Hatini et al., 2013, Sotillos et al., 2013, Wu et al., 2010, Flaherty et al., 2009, Betz et al., 2008, Sotillos et al., 2008, Ekas et al., 2006, Henriksen et al., 2002)

stat92e³⁹⁷

(Santabárbara-Ruiz et al., 2015)

stat^397-6

(Gao et al., 2009)

stat³⁹⁷

(Jiang et al., 2011, Yoon et al., 2011, Krzemień et al., 2007, Silver et al., 2005)

Name Synonyms

Secondary FlyBase IDs

References (27)

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