FlyBase Allele Report: Dmel\upd3[d232a]

General Information

Symbol

Dmel\upd3^d232a

Species

D. melanogaster

Name

FlyBase ID

FBal0302832

Feature type

allele

Associated gene

Dmel\upd3

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Wang et al., 2014)

Progenitor genotype

upd3^d00871

(Wang et al., 2014)

Cytology

Description

Imprecise excision of the P{XP} element in P{XP}upd3^d00871 has removed the entire last exon of upd3.

(Wang et al., 2014)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal immune response

(Wang et al., 2014)

female fertile

(Wang et al., 2014)

male fertile

(Wang et al., 2014)

male sterile | progressive

(Wang et al., 2014)

visible

(Wang et al., 2014)

visible (with Ab(1)os^s)

(Wang et al., 2014)

visible (with Df(1)os1A2)

(Wang et al., 2014)

visible (with Df(1)os¹)

(Wang et al., 2014)

visible (with Df(1)os^o)

(Wang et al., 2014)

visible (with upd3^x21b)

(Wang et al., 2014)

Phenotype Manifest In

border follicle cell

(Wang et al., 2014)

eye

(Wang et al., 2014)

eye (with Ab(1)os^s)

(Wang et al., 2014)

eye (with Df(1)os1A2)

(Wang et al., 2014)

eye (with Df(1)os¹)

(Wang et al., 2014)

eye (with Df(1)os^o)

(Wang et al., 2014)

eye (with upd3^x21b)

(Wang et al., 2014)

haltere

(Wang et al., 2014)

wing

(Wang et al., 2014)

wing (with Df(1)os1A2)

(Wang et al., 2014)

wing (with Df(1)os¹)

(Wang et al., 2014)

wing (with upd3^x21b)

(Wang et al., 2014)

Detailed Description

Statement

Reference

The eyes of upd3^d232a mutant flies are reduced in size compared to controls occasionally resulting in complete eye loss. The number of ommatidia is reduced, but most remaining facets appear normal. Missing ommatidia appear to be replaced by head cuticle, resulting in increased bare cuticle between the bristles surrounding the eye and the ommatidia.

upd3^d232a mutant flies display outstretched wings.

In contrast to wild type flies, upd3^d232a mutant flies hold their halteres down.

The egg chambers in upd3^d232a mutant ovaries contain significantly fewer border cells than controls. A greater proportion of micropyles from upd3^d232a mutant females have blocked or missing channels. The length of the micropyle is shorter on average in eggs from upd3^d232a mutant females. The specification of both anterior and posterior terminal follicle cells is impaired. Young females contain a similar number of fused egg chambers to wild type, but the proportion increases with age compared to the controls.

A greater proportion of embryos from upd3^d232a mutant mothers fail to hatch compared to wild type. Many of the unhatched eggs are unfertilized.

upd3^d232a mutant males become infertile at a much younger age than wild-type controls.

upd3^d232a mutant larvae have a significantly higher rate of parasite encapsulation than wild-type controls when infected with the wasp L.boullardi. The differentiation of lamellocytes is unaffected. No reduction in survival is observed when either adults or larvae are subjected to infection by E.coli.

upd3^d232a/upd3^x21b mutant flies have outstretched wings and small eyes compared to wild type.

upd3^d232a/Df(1)os1A2 mutant flies have outstretched wings and small eyes compared to wild type.

Df(1)os¹/upd3^d232a mutant flies have outstretched wings and small eyes compared to wild type.

Ab(1)os^s/upd3^d232a mutant flies have small eyes compared to wild type. No wing defects are seen.

Df(1)os^o/upd3^d232a mutant flies have outstretched wings. Eye size is similar to wild type.

(Wang et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

upd3^d232a has visible phenotype, enhanceable by Stat92E³⁹⁷/Stat92E[+]

(Wang et al., 2014)

upd3^d232a has visible phenotype, enhanceable by Stat92E^85C9/Stat92E[+]

(Wang et al., 2014)

NOT Enhanced by

Statement

Reference

upd3^d232a has visible phenotype, non-enhanceable by Stat92E³⁹⁷/Stat92E[+]

(Wang et al., 2014)

upd3^d232a has visible phenotype, non-enhanceable by Stat92E^85C9/Stat92E[+]

(Wang et al., 2014)

NOT suppressed by

Statement

Reference

upd3^d232a has visible phenotype, non-suppressible by Socs36E[+]/Socs36E^189a

(Wang et al., 2014)

Phenotype Manifest In

Enhanced by

Statement

Reference

upd3^d232a has wing phenotype, enhanceable by Stat92E³⁹⁷/Stat92E[+]

(Wang et al., 2014)

upd3^d232a has haltere phenotype, enhanceable by Socs36E[+]/Socs36E^189a

(Wang et al., 2014)

upd3^d232a has haltere phenotype, enhanceable by Stat92E³⁹⁷/Stat92E[+]

(Wang et al., 2014)

upd3^d232a has wing phenotype, enhanceable by Stat92E^85C9/Stat92E[+]

(Wang et al., 2014)

upd3^d232a has haltere phenotype, enhanceable by Stat92E^85C9/Stat92E[+]

(Wang et al., 2014)

NOT Enhanced by

Statement

Reference

upd3^d232a has eye phenotype, non-enhanceable by Stat92E³⁹⁷/Stat92E[+]

(Wang et al., 2014)

upd3^d232a has eye phenotype, non-enhanceable by Stat92E^85C9/Stat92E[+]

(Wang et al., 2014)

NOT suppressed by

Statement

Reference

upd3^d232a has eye phenotype, non-suppressible by Socs36E[+]/Socs36E^189a

(Wang et al., 2014)

upd3^d232a has wing phenotype, non-suppressible by Socs36E[+]/Socs36E^189a

(Wang et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

One copy of Stat92E³⁹⁷ modestly but significantly enhances the reduction in eye size seen in upd3^d232a mutant flies, but the reduction in eye size is similar to that seen in Stat92E³⁹⁷ mutants alone.

One copy of Stat92E³⁹⁷ strongly enhances the outstretched wing and held down haltere phenotypes seen in upd3^d232a mutant flies.

One copy of Stat92E^85C9 modestly but significantly enhances the reduction in eye size seen in upd3^d232a mutant flies, but the reduction in eye size is similar to that seen in Stat92E^85C9 mutants alone.

One copy of Stat92E^85C9 strongly enhances the outstretched wing and held down haltere phenotypes seen in upd3^d232a mutant flies.

One copy of Socs36E^189a modestly but significantly suppresses the reduction in eye size seen in upd3^d232a mutant flies. but the increase in eye size is no greater than that seen in Socs36E^189a mutant flies alone.

One copy of Socs36E^189a has no effect on the outstretched wing phenotype seen in upd3^d232a mutant flies.

One copy of Socs36E^189a enhances the held out haltere phenotype seen in upd3^d232a mutant flies.

upd3^x21b/Df(1)os^o mutant flies have outstretched wings. Eye size is similar to wild type.

(Wang et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

upd3^d232a is rescued by upd3^UAS.GFP/Scer\GAL4^upd3.PA

(Wang et al., 2014)

Not rescued by

upd3^d232a is not rescued by upd3^t13

(Wang et al., 2014)

Comments

Expression of upd3^t13 is unable to rescue the eye and wing phenotypes seen in upd3^d232a mutant flies.

Expression of upd3^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^upd3.PA rescues the reduction in eye size seen in upd3^d232a mutant males.

(Wang et al., 2014)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

upd3^d232a

(Wang et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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