FB2024_03 , released June 25, 2024
Allele: Dmel\slik04807b
Open Close
General Information
Symbol
Dmel\slik04807b
Species
D. melanogaster
Name
disrupted innervation
FlyBase ID
FBal0129067
Feature type
allele
Associated gene
Dmel\sli
Associated Insertion(s)
P{lacW}slik04807b
Carried in Construct
Also Known As
slidui, slitdui
Key Links
Allele class

hypomorphic allele - molecular evidence

loss of function allele

Mutagen
    Nature of the Allele
    Allele class

    hypomorphic allele - molecular evidence

    (Caipo et al., 2020)

    loss of function allele

    (Tayler et al., 2004)
    Mutagen
    Progenitor genotype
    Associated Insertion(s)
    P{lacW}slik04807b
    Cytology
    Description

    P{lacW} insertion 29,404bp upstream of the 5' end of the sli transcript, with the Ecol\lacZ coding region oriented toward the sli locus.

    (Tayler et al., 2004)
    Allele components
    Component
    Use(s)
    Inserted element
    P{lacW}
    Encoded product / tool
    lacZ
    Tagged with
    Tag:NLS(P)
    Mutations Mapped to the Genome
    Curation Data
    Type
    Location
    Additional Notes
    References
    Variant Molecular Consequences
    Associated Sequence Data
    DDBJ / EMBL / GenBank
    DNA sequence
    Protein sequence
     
    UniProtKB/Swiss-Prot
      UniProtKB/TrEMBL
        Expression Data
        Reporter Expression
        Additional Information
        Statement
        Reference
         
        Marker for
        Reflects expression of
        Reporter construct used in assay
        Human Disease Associations
        Disease Ontology (DO) Annotations
        Models Based on Experimental Evidence ( 0 )
        Disease
        Evidence
        References
        Modifiers Based on Experimental Evidence ( 0 )
        Disease
        Interaction
        References
        Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
         
        Disease-implicated variant(s)
         
        Phenotypic Data
        Phenotypic Class
        Phenotype Manifest In

        adult mushroom body

        (Oliva et al., 2016)

        adult mushroom body (with sli2)

        (Oliva et al., 2016)

        adult optic lobe

        (Caipo et al., 2020)

        adult optic lobe (with sli2)

        (Caipo et al., 2020)

        adult s-LNv neuron | adult stage

        (Oliva et al., 2016)

        adult s-LNv neuron | adult stage (with sli2)

        (Oliva et al., 2016)

        axon | adult stage

        (Oliva et al., 2016)

        axon | adult stage (with sli2)

        (Oliva et al., 2016)

        axon | P-stage

        (Caipo et al., 2020)

        chiasma

        (Pappu et al., 2011)

        ellipsoid body | adult stage

        (Oliva et al., 2016)

        eye photoreceptor cell

        (Pappu et al., 2011)

        eye photoreceptor cell & axon (with sli2)

        (Tayler et al., 2004)

        eye photoreceptor cell & axon (with sli05248)

        (Tayler et al., 2004)

        eye photoreceptor cell & axon (with sliE-158)

        (Tayler et al., 2004)

        eye photoreceptor cell | P-stage

        (Caipo et al., 2020)

        lamina anlage

        (Tayler et al., 2004)

        lamina anlage (with sli2)

        (Tayler et al., 2004)

        lamina anlage (with sli05248)

        (Tayler et al., 2004)

        lamina anlage (with sliE-158)

        (Tayler et al., 2004)

        lamina anlage glial cell (with sli2)

        (Tayler et al., 2004)

        larval inner optic anlage | ectopic | third instar larval stage

        (Caipo et al., 2020)

        larval neuroblast | third instar larval stage

        (Caipo et al., 2020)

        lobula plate | adult stage

        (Caipo et al., 2020)

        medulla | adult stage

        (Caipo et al., 2020)

        medulla | P-stage

        (Caipo et al., 2020)

        medulla | third instar larval stage

        (Caipo et al., 2020)

        neuron projection | adult stage | ectopic

        (Caipo et al., 2020)

        neuropil | adult stage (with sli2)

        (Oliva et al., 2016)

        photoreceptor cell R1 & axon

        (Tayler et al., 2001)

        photoreceptor cell R2 & axon

        (Tayler et al., 2004, Tayler et al., 2001)

        photoreceptor cell R3 & axon

        (Tayler et al., 2004, Tayler et al., 2001)

        photoreceptor cell R4 & axon

        (Tayler et al., 2004, Tayler et al., 2001)

        photoreceptor cell R5 & axon

        (Tayler et al., 2004, Tayler et al., 2001)

        photoreceptor cell R6 & axon

        (Tayler et al., 2001)

        T neuron | adult stage | ectopic

        (Caipo et al., 2020)
        Detailed Description
        Statement
        Reference

        slik04807b homozygosity leads to ectopic localization of inner proliferating center (IPC) neuroblasts in the medulla of third instar larvae; defects in optic lobe organization with an absence of the organization of photoreceptor cell axons in pupae; and elongated optic lobes with architecture defects (such as absence of organization of the medulla and elongated lobula plate but no changes in the organization of lobula or lamina), ectopic projections in the medulla (likely to be originated from lamina neurons), ectopic localization of IPC-derived cell populations (such as lobula plate T4/T5 neurons) misplaced in the proximal medulla and incorrect connection of the lamina to the medulla through its proximal region (next to the lobula plate) instead of connecting through the distal medulla in adults when compared to controls.

        slik04807b homozygous adults exhibit defects in response to the light compared to controls.

        slik04807b heterozygous adults do not exhibit any optic lobe neuropil defects when compared to controls.

        (Caipo et al., 2020)

        The adult brain of sli2/slik04807b transheterozygotes exhibits major defects in neuropil organization in several central brain areas including the central complex, mushroom body lobes, antennal lobes and Robo2/Robo3-expressing axons, as compared to either heterozygous controls; their adult sLNv neurons exhibit significantly longer axon projections compared to controls.

        The adult brain of slik04807b homozygotes exhibits defects in the organization of the ellipsoid body and the mushroom body, and their adult sLNv neurons exhibit significantly longer axon projections, as compared to controls.

        (Oliva et al., 2016)

        Photoreceptor cell axons do not form an optic chiasm in slik04807b mutants.

        (Pappu et al., 2011)

        There are gaps in the lamina neuropil in the visual system of homozygous third instar larvae and increased numbers of photoreceptor axons enter the medulla; many R2-R5 axons pass through the lamina and enter the medulla, in contrast to wild type where they stop in the lamina neuropil. slik04807b/sli05248, slik04807b/sliE-158 and slik04807b/sli2 third instar larvae show defects in photoreceptor axon targeting, with gaps in the lamina and increased numbers of axons entering the medulla. slik04807b/sli2 animals show disruption of the lamina glia layer; clumps of glia and gaps in the glial layers are seen. The regions of photoreceptor axon mistargeting correlate with areas of lamin glial disruption. Many distal cell neurons enter the base of the lamina in slik04807b/sli2 third instar larvae and some distal cell neurons invade the lamina neuropil, disrupting photoreceptor innervation.

        (Tayler et al., 2004)

        Mutant strongly disrupts the target layer selections of R1-R6 photoreceptor axons.

        (Tayler et al., 2001)
        External Data
        Interactions
        Show genetic interaction network for Enhancers & Suppressors
        Phenotypic Class
        NOT Enhancer of
        Statement
        Reference

        sli[+]/slik04807b is a non-enhancer of abnormal neuroanatomy phenotype of egh7

        (Fan et al., 2005)
        Suppressor of
        NOT Suppressor of
        Statement
        Reference

        sli[+]/slik04807b is a non-suppressor of abnormal neuroanatomy phenotype of egh7

        (Fan et al., 2005)
        Phenotype Manifest In
        NOT Enhancer of
        Statement
        Reference

        sli[+]/slik04807b is a non-enhancer of photoreceptor cell R1 & axon phenotype of egh7

        (Fan et al., 2005)

        sli[+]/slik04807b is a non-enhancer of photoreceptor cell R2 & axon phenotype of egh7

        (Fan et al., 2005)

        sli[+]/slik04807b is a non-enhancer of photoreceptor cell R3 & axon phenotype of egh7

        (Fan et al., 2005)

        sli[+]/slik04807b is a non-enhancer of photoreceptor cell R4 & axon phenotype of egh7

        (Fan et al., 2005)

        sli[+]/slik04807b is a non-enhancer of photoreceptor cell R5 & axon phenotype of egh7

        (Fan et al., 2005)

        sli[+]/slik04807b is a non-enhancer of photoreceptor cell R6 & axon phenotype of egh7

        (Fan et al., 2005)
        Suppressor of
        NOT Suppressor of
        Statement
        Reference

        sli[+]/slik04807b is a non-suppressor of photoreceptor cell R1 & axon phenotype of egh7

        (Fan et al., 2005)

        sli[+]/slik04807b is a non-suppressor of photoreceptor cell R2 & axon phenotype of egh7

        (Fan et al., 2005)

        sli[+]/slik04807b is a non-suppressor of photoreceptor cell R3 & axon phenotype of egh7

        (Fan et al., 2005)

        sli[+]/slik04807b is a non-suppressor of photoreceptor cell R4 & axon phenotype of egh7

        (Fan et al., 2005)

        sli[+]/slik04807b is a non-suppressor of photoreceptor cell R5 & axon phenotype of egh7

        (Fan et al., 2005)

        sli[+]/slik04807b is a non-suppressor of photoreceptor cell R6 & axon phenotype of egh7

        (Fan et al., 2005)
        Additional Comments
        Genetic Interactions
        Statement
        Reference

        The expression of robo2UAS.EGFP leads to shorter sLNv neuron projections (Scer\GAL4Pdf.PU-driven expression) and to the absence of the normal DCN neuron commissure (Scer\GAL4ato.PU-driven expression), both of which are suppressed by sli2/slik04807b transheterozygosity.

        (Oliva et al., 2016)

        In egh7/Y; slik04807b/+ mutants, the R-cell axonal projection pattern is defective like in egh7 single mutants. egh7/+; slik04807b/+ mutants appear wild type.

        (Fan et al., 2005)
        Xenogenetic Interactions
        Statement
        Reference
        Complementation and Rescue Data
        Images (0)
        Mutant
        Wild-type
        Stocks (1)
        Notes on Origin
        Discoverer

        Separable from: P{lacW}k04807a.

        (Tayler et al., 2004)
        Comments
        Comments

        Precise excision of the P{lacW} element reverts the mutant phenotype.

        (Tayler et al., 2004)
        External Crossreferences and Linkouts ( 0 )
        Synonyms and Secondary IDs (4)
        Reported As
        Symbol Synonym
        slidui
        (Caipo et al., 2020, Suzuki et al., 2018, Oliva et al., 2016, Fan et al., 2005, Tayler et al., 2004)
        slik04807b
        slitdui
        (González-Ramírez et al., 2022, Plazaola-Sasieta et al., 2019)
        Name Synonyms
        disrupted innervation
        (Tayler et al., 2004, Tayler et al., 2001)
        Secondary FlyBase IDs
          References (9)