FlyBase Allele Report: Dmel\egh[7]

General Information

Symbol

Dmel\egh⁷

Species

D. melanogaster

Name

FlyBase ID

FBal0006837

Feature type

allele

Associated gene

Dmel\egh

Associated Insertion(s)

Carried in Construct

Also Known As

egh^65h5

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Soller et al., 2006)

Mutagen

X ray

ethylenimine

ethyl methanesulfonate

(Goode et al., 1996)

Progenitor genotype

Cytology

Description

Nucleotide substitution: A?T.

(Soller et al., 2006)

The A to T transversion results in the M308K substitution. T347C and T409C are point mutations in the UTR of exon 2 and there is also a C added at position 278.

(Soller et al., 2006)

Nucleotide substitution: T347C.

(Soller et al., 2006)

Nucleotide substitution: T409C.

(Soller et al., 2006)

Amino acid replacement: M308K.

(Soller et al., 2006, Wandall et al., 2005)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:2,597,448..2,597,448 [+]

Nucleotide change:

T2597448A

Amino acid change:

M308K | egh-PB; M308K | egh-PC; M308K | egh-PD

Reported amino acid change:

M308K

Comment:

Site of nucleic acid difference inferred by FlyBase based on reported amino acid change.

(Wandall et al., 2005)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

dorsal appendage | germline clone

(Goode et al., 1996)

egg chamber | germline clone

(Goode et al., 1996)

embryonic/larval neuromuscular junction (with egh^62d18)

(Huang et al., 2018)

embryonic/larval optic lobe

(Fan et al., 2005)

embryonic/larval peripheral nervous system | third instar larval stage

(Dahlgaard et al., 2012)

embryonic/larval plasmatocyte | ectopic

(Dahlgaard et al., 2012)

female germline cyst | germline clone

(Goode et al., 1996)

follicle cell | germline clone

(Goode et al., 1996)

lamina anlage

(Fan et al., 2005)

lamina anlage glial cell

(Fan et al., 2005)

lamina plexus

(Fan et al., 2005)

larval abdominal segment 9 | third instar larval stage

(Dahlgaard et al., 2012)

larval ventral nerve cord (with egh^cm)

(Soller et al., 2006)

larval ventral nerve cord | somatic clone

(Soller et al., 2006)

larval ventral nerve cord | third instar larval stage

(Dahlgaard et al., 2012)

lobula

(Fan et al., 2005)

lobula & neuron

(Fan et al., 2005)

nurse cell | germline clone

(Goode et al., 1996)

oocyte | germline clone

(Goode et al., 1996)

photoreceptor cell R1 & axon

(Fan et al., 2005)

photoreceptor cell R2 & axon

(Fan et al., 2005)

photoreceptor cell R3 & axon

(Fan et al., 2005)

photoreceptor cell R4 & axon

(Fan et al., 2005)

photoreceptor cell R5 & axon

(Fan et al., 2005)

photoreceptor cell R6 & axon

(Fan et al., 2005)

presumptive embryonic/larval nervous system | germline clone

(Goode et al., 1996)

stalk follicle cell | germline clone

(Goode et al., 1996)

terminal bouton | larval stage (with egh^62d18)

(Huang et al., 2018)

Detailed Description

Statement

Reference

egh^62d18/egh⁷ larvae have significantly reduced bouton number and significantly increased bouton size at the neuromuscular junction of muscle 4, compared to controls, with no effect on the number of satellite boutons.

(Huang et al., 2018)

egh⁷ mutant third instar larvae exhibit enlargement of peripheral nerves attached immune cells. This is most pronounced in the peripheral nerves that project from the most caudal segment (A9) of the ventral nerve cord. The immune cells are identified as plasmatocytes, that are normally circulating in the hemolymph. In severe cases, large plasmatocyte aggregates completely enfold the nerves in the mutants. There is no obvious correlation between nerve diameter and the degree of plasmatocyte accumulation in the mutants. Neither nerve growth nor plasmatocyte accumulation is observed in wild-type larvae.

(Dahlgaard et al., 2012)

egh^cm/egh⁷ females are insensitive to injection with sex peptide; oviposition is not induced in these females and mating is not suppressed.

Remating in egh^cm and egh^cm/egh⁷ females is about 48% and 24% compared to mating of virgin egh^cm and egh^cm/egh⁷ females, which is a higher amount than in control females. Sex appeal of mated egh^cm/egh⁷ females to males is reduced to the same extent as control mated females.

egh⁷ clones in the VNC Ap neurons do not extend neuronal projections to the anterior part of the central brain and fail to form the elaborate arborizations that are observed in wild-type flies.

In larval brains of egh^cm/egh⁷ mutants, Ap neurons projecting from the VNC to the CNS show pathfinding errors and fail to reach the target area. In many cases, these axons defasciculate and turn backward. Mostly, these axons stop extending toward the central brain but rarely defasciculate.

(Soller et al., 2006)

In the brains of egh⁷ third instar larvae, some of the axons of R-cells 1-6 fail to terminate in the lamina and project into the medulla, causing thick axon bundles in this structure. R1-R6 axons that do terminate in the lamina are disorganized and form abnormal patches in the lamina plexus. In egh⁷ mutants, the boundary region that exists in wild type between the lobula and lamina is no longer apparent and the sheath-like glial processes that usually delimit this boundary are severely disrupted or missing altogether. This allows lobula distal neurons to cross into the base of the developing lamina, disrupt the pattern of lamina glia and result in the perturbed R1-R6 axonal projections. Clonal analysis shows that the egh⁷ is required in cells of the lobula complex primordium, as egh⁷ mutant clones in this structure are associated the same perturbed R1-R6 axonal projection phenotype as egh⁷ flies. However, egh⁷ clones in the eye, glial cells, lamina precursors, or lamina neurons do not cause this phenotype.

(Fan et al., 2005)

Females with germline clones lay eggs with fused dorsal appendages and the embryos have an expanded nervous system. Only a few eggs are laid because early oogenesis is blocked due to the development of egg chambers containing multiple oocyte-nurse cell complexes. Germ cell divisions in the egg chambers are not regulated. Follicle cells at the posterior of egg chamber become mesenchymal-like indicating they have not adopted a border cell fate. Stalk cells are determined correctly but are usually disorganised compared to wild type.

(Goode et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

egh⁷ has abnormal neuroanatomy phenotype, non-enhanceable by Scer\GAL4^elav-C155/robo1^RNAi.UAS

(Fan et al., 2005)

egh⁷ has abnormal neuroanatomy phenotype, non-enhanceable by sli[+]/sli^k04807b

(Fan et al., 2005)

egh⁷ has abnormal neuroanatomy phenotype, non-enhanceable by sli²/sli[+]

(Fan et al., 2005)

Suppressed by

Statement

Reference

egh⁷ has abnormal neuroanatomy phenotype, suppressible by Hsap\B4GALT6^UAS.cWa/Scer\GAL4^arm.PS

(Fan et al., 2005)

NOT suppressed by

Statement

Reference

egh⁷ has abnormal neuroanatomy phenotype, non-suppressible by Scer\GAL4^elav-C155/robo1^RNAi.UAS

(Fan et al., 2005)

egh⁷ has abnormal neuroanatomy phenotype, non-suppressible by sli[+]/sli^k04807b

(Fan et al., 2005)

egh⁷ has abnormal neuroanatomy phenotype, non-suppressible by sli²/sli[+]

(Fan et al., 2005)

NOT Enhancer of

Statement

Reference

egh⁷ is a non-enhancer of visible phenotype of H^UAS.cMa, Scer\GAL4^GMR.PU

(Protzer et al., 2009)

NOT Suppressor of

Statement

Reference

egh⁷ is a non-suppressor of visible phenotype of H^UAS.cMa, Scer\GAL4^GMR.PU

(Protzer et al., 2009)

Other

Statement

Reference

egh⁷, hryⁱ²²/h[+] has partially lethal phenotype

(Bianchi-Frias et al., 2004)

egh⁷, h[+]/hry²⁶ has partially lethal phenotype

(Bianchi-Frias et al., 2004)

egh⁷, hry²⁶ has partially lethal phenotype

(Bianchi-Frias et al., 2004)

egh⁷, hryⁱ²² has partially lethal phenotype

(Bianchi-Frias et al., 2004)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

egh⁷ has photoreceptor cell R1 & axon phenotype, non-enhanceable by Scer\GAL4^elav-C155/robo1^RNAi.UAS

(Fan et al., 2005)

egh⁷ has photoreceptor cell R3 & axon phenotype, non-enhanceable by sli²/sli[+]

(Fan et al., 2005)

egh⁷ has photoreceptor cell R4 & axon phenotype, non-enhanceable by sli²/sli[+]

(Fan et al., 2005)

egh⁷ has photoreceptor cell R5 & axon phenotype, non-enhanceable by sli²/sli[+]

(Fan et al., 2005)

egh⁷ has photoreceptor cell R6 & axon phenotype, non-enhanceable by sli²/sli[+]

(Fan et al., 2005)

egh⁷ has photoreceptor cell R1 & axon phenotype, non-enhanceable by sli[+]/sli^k04807b

(Fan et al., 2005)

egh⁷ has photoreceptor cell R2 & axon phenotype, non-enhanceable by sli[+]/sli^k04807b

(Fan et al., 2005)

egh⁷ has photoreceptor cell R3 & axon phenotype, non-enhanceable by sli[+]/sli^k04807b

(Fan et al., 2005)

egh⁷ has photoreceptor cell R4 & axon phenotype, non-enhanceable by sli[+]/sli^k04807b

(Fan et al., 2005)

egh⁷ has photoreceptor cell R5 & axon phenotype, non-enhanceable by sli[+]/sli^k04807b

(Fan et al., 2005)

egh⁷ has photoreceptor cell R2 & axon phenotype, non-enhanceable by Scer\GAL4^elav-C155/robo1^RNAi.UAS

(Fan et al., 2005)

egh⁷ has photoreceptor cell R6 & axon phenotype, non-enhanceable by sli[+]/sli^k04807b

(Fan et al., 2005)

egh⁷ has photoreceptor cell R3 & axon phenotype, non-enhanceable by Scer\GAL4^elav-C155/robo1^RNAi.UAS

(Fan et al., 2005)

egh⁷ has photoreceptor cell R4 & axon phenotype, non-enhanceable by Scer\GAL4^elav-C155/robo1^RNAi.UAS

(Fan et al., 2005)

egh⁷ has photoreceptor cell R5 & axon phenotype, non-enhanceable by Scer\GAL4^elav-C155/robo1^RNAi.UAS

(Fan et al., 2005)

egh⁷ has photoreceptor cell R6 & axon phenotype, non-enhanceable by Scer\GAL4^elav-C155/robo1^RNAi.UAS

(Fan et al., 2005)

egh⁷ has photoreceptor cell R1 & axon phenotype, non-enhanceable by sli²/sli[+]

(Fan et al., 2005)

egh⁷ has photoreceptor cell R2 & axon phenotype, non-enhanceable by sli²/sli[+]

(Fan et al., 2005)

Suppressed by

Statement

Reference

egh⁷ has photoreceptor cell R1 & axon phenotype, suppressible by Hsap\B4GALT6^UAS.cWa/Scer\GAL4^arm.PS

(Fan et al., 2005)

egh⁷ has photoreceptor cell R2 & axon phenotype, suppressible by Hsap\B4GALT6^UAS.cWa/Scer\GAL4^arm.PS

(Fan et al., 2005)

egh⁷ has photoreceptor cell R3 & axon phenotype, suppressible by Hsap\B4GALT6^UAS.cWa/Scer\GAL4^arm.PS

(Fan et al., 2005)

egh⁷ has photoreceptor cell R4 & axon phenotype, suppressible by Hsap\B4GALT6^UAS.cWa/Scer\GAL4^arm.PS

(Fan et al., 2005)

egh⁷ has photoreceptor cell R5 & axon phenotype, suppressible by Hsap\B4GALT6^UAS.cWa/Scer\GAL4^arm.PS

(Fan et al., 2005)

egh⁷ has photoreceptor cell R6 & axon phenotype, suppressible by Hsap\B4GALT6^UAS.cWa/Scer\GAL4^arm.PS

(Fan et al., 2005)

NOT suppressed by

Statement

Reference

egh⁷ has photoreceptor cell R3 & axon phenotype, non-suppressible by sli²/sli[+]

(Fan et al., 2005)

egh⁷ has photoreceptor cell R3 & axon phenotype, non-suppressible by sli[+]/sli^k04807b

(Fan et al., 2005)

egh⁷ has photoreceptor cell R4 & axon phenotype, non-suppressible by Scer\GAL4^elav-C155/robo1^RNAi.UAS

(Fan et al., 2005)

egh⁷ has photoreceptor cell R4 & axon phenotype, non-suppressible by sli²/sli[+]

(Fan et al., 2005)

egh⁷ has photoreceptor cell R4 & axon phenotype, non-suppressible by sli[+]/sli^k04807b

(Fan et al., 2005)

egh⁷ has photoreceptor cell R5 & axon phenotype, non-suppressible by Scer\GAL4^elav-C155/robo1^RNAi.UAS

(Fan et al., 2005)

egh⁷ has photoreceptor cell R5 & axon phenotype, non-suppressible by sli²/sli[+]

(Fan et al., 2005)

egh⁷ has photoreceptor cell R5 & axon phenotype, non-suppressible by sli[+]/sli^k04807b

(Fan et al., 2005)

egh⁷ has photoreceptor cell R6 & axon phenotype, non-suppressible by Scer\GAL4^elav-C155/robo1^RNAi.UAS

(Fan et al., 2005)

egh⁷ has photoreceptor cell R6 & axon phenotype, non-suppressible by sli²/sli[+]

(Fan et al., 2005)

egh⁷ has photoreceptor cell R6 & axon phenotype, non-suppressible by sli[+]/sli^k04807b

(Fan et al., 2005)

egh⁷ has photoreceptor cell R1 & axon phenotype, non-suppressible by Scer\GAL4^elav-C155/robo1^RNAi.UAS

(Fan et al., 2005)

egh⁷ has photoreceptor cell R1 & axon phenotype, non-suppressible by sli²/sli[+]

(Fan et al., 2005)

egh⁷ has photoreceptor cell R1 & axon phenotype, non-suppressible by sli[+]/sli^k04807b

(Fan et al., 2005)

egh⁷ has photoreceptor cell R2 & axon phenotype, non-suppressible by Scer\GAL4^elav-C155/robo1^RNAi.UAS

(Fan et al., 2005)

egh⁷ has photoreceptor cell R2 & axon phenotype, non-suppressible by sli²/sli[+]

(Fan et al., 2005)

egh⁷ has photoreceptor cell R2 & axon phenotype, non-suppressible by sli[+]/sli^k04807b

(Fan et al., 2005)

egh⁷ has photoreceptor cell R3 & axon phenotype, non-suppressible by Scer\GAL4^elav-C155/robo1^RNAi.UAS

(Fan et al., 2005)

NOT Enhancer of

Statement

Reference

egh⁷ is a non-enhancer of eye phenotype of H^UAS.cMa, Scer\GAL4^GMR.PU

(Protzer et al., 2009)

NOT Suppressor of

Statement

Reference

egh⁷ is a non-suppressor of eye phenotype of H^UAS.cMa, Scer\GAL4^GMR.PU

(Protzer et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

The eye phenotype caused by expression of H^Scer\UAS.cMa under the control of Scer\GAL4^GMR.PU is unaffected if the flies also carry egh⁷.

(Protzer et al., 2009)

In egh⁷/Y; sli²/+ and egh⁷/Y; sli^k04807b/+ mutants, the R-cell axonal projection pattern is defective like in egh⁷ single mutants. When egh⁷ is heterozygous in these double mutants, flies appear wild type. Expression of robo^{dsRNA.Scer\UAS}, under the control of Scer\GAL4^elav-C155, does not alter the R-cell phenotype of egh⁷ mutants.

(Fan et al., 2005)

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\β4Gal-T6^Scer\UAS.cWa, under the control of Scer\GAL4^arm.PS, in egh⁷ mutants rescues the R-cell axonal projection phenotype.

(Fan et al., 2005)

Complementation and Rescue Data

Rescued by

egh⁷/egh^cm is rescued by egh^+tP2

(Soller et al., 2006)

egh⁷/egh^cm is rescued by Scer\GAL4^elav-C155/egh^UAS.Tag:HA

(Soller et al., 2006)

egh⁷ is rescued by egh^+tP2

(Soller et al., 2006)

egh⁷ is rescued by egh^UAS.Tag:HA/Scer\GAL4^Tub.PU

(Soller et al., 2006)

egh⁷ is rescued by Scer\GAL4^elav-C155/egh^UAS.Tag:HA

(Soller et al., 2006)

egh⁷ is rescued by Scer\GAL4^αTub84B.PL/egh^UAS.cFa

(Fan et al., 2005)

egh⁷ is rescued by egh^hs.PG

(Goode et al., 1996)

egh⁷ is rescued by egh^+t7

(Goode et al., 1996)

egh⁷ is rescued by egh^+t7

(Williams et al., 1995)

Partially rescued by

egh⁷/egh^cm is partially rescued by egh^UAS.Tag:HA/Scer\GAL4^Tub.PU

(Soller et al., 2006)

egh⁷/egh^cm is partially rescued by egh^UAS.Tag:HA/Scer\GAL4^ap-md544

(Soller et al., 2006)

egh⁷/egh^cm is partially rescued by Scer\GAL4^tsh-md621/egh^UAS.Tag:HA

(Soller et al., 2006)

egh⁷ is partially rescued by egh^P1

(Soller et al., 2006)

egh⁷ is partially rescued by Scer\GAL4^tsh-md621/egh^UAS.Tag:HA

(Soller et al., 2006)

egh⁷ is partially rescued by Scer\GAL4^elav-C155/egh^UAS.cFa

(Fan et al., 2005)

Not rescued by

egh⁷ is not rescued by egh^UAS.Tag:MYC/Scer\GAL4^C57

(Huang et al., 2018)

egh⁷ is not rescued by egh^UAS.Tag:MYC/Scer\GAL4^repo

(Huang et al., 2018)

egh⁷/egh^cm is not rescued by egh^UAS.Tag:HA/Scer\GAL4^gcm-rA87.C

(Soller et al., 2006)

egh⁷/egh^cm is not rescued by egh^UAS.Tag:HA/Scer\GAL4^repo

(Soller et al., 2006)

egh⁷/egh^cm is not rescued by egh^UAS.Tag:HA/Scer\GAL4^elav.PLu

(Soller et al., 2006)

egh⁷ is not rescued by egh^UAS.Tag:HA/Scer\GAL4^gcm-rA87.C

(Soller et al., 2006)

egh⁷ is not rescued by egh^UAS.Tag:HA/Scer\GAL4^repo

(Soller et al., 2006)

egh⁷ is not rescued by egh^UAS.Tag:HA/Scer\GAL4^elav.PLu

(Soller et al., 2006)

egh⁷ is not rescued by egh^UAS.Tag:HA/Scer\GAL4^ap-md544

(Soller et al., 2006)

egh⁷ is not rescued by Scer\GAL4^1.3D2/egh^UAS.cFa

(Fan et al., 2005)

egh⁷ is not rescued by Scer\GAL4^GMR.PF/egh^UAS.cFa

(Fan et al., 2005)

egh⁷ is not rescued by Scer\GAL4^gcm-rA87.C/egh^UAS.cFa

(Fan et al., 2005)

Comments

Expression of egh^{Scer\UAS.T:Ivir\HA1} under the control of either Scer\GAL4^ap-md544 or Scer\GAL4^tsh-md621 rescues the egg retention phenotype of egh^cm/egh⁷ females. Most egh^cm/egh⁷ larval brains show a complete rescue of the ventral nerve cord defect when egh^{Scer\UAS.T:Ivir\HA1} in expressed under the control of Scer\GAL4^ap-md544.

Expression of egh^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^tsh-md621 rescues the viability of egh⁷ flies but these flies are short lived. Scer\GAL4^ap-md544>egh^{Scer\UAS.T:Ivir\HA1} expression does not rescue egh⁷ viability.

Expression of egh^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^tsh-md621 rescues the receptivity response of egh^cm/egh⁷ females to sex peptide completely. This response is partially rescued when egh^{Scer\UAS.T:Ivir\HA1} is expressed under the control of either Scer\GAL4^tub or Scer\GAL4^ap-md544, but is not rescued when Scer\GAL4^elav.PLu is used to drive expression. Oviposition after treatment with sex peptide is rescued in 67% of egh^cm/egh⁷ females (the other 33% lay no eggs) when egh^{Scer\UAS.T:Ivir\HA1} is expressed under the control of Scer\GAL4^ap-md544 and is rescued to wild-type levels when driven by either Scer\GAL4^tub or Scer\GAL4^tsh-md621. Expression of egh^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^elav.PLu does not rescue oviposition.

(Soller et al., 2006)

Expression of egh^Scer\UAS.cFa under the control of Scer\GAL4^elav-C155 rescues the R-cell projection phenotype in 80% of mutant egh⁷ brain hemispheres tested.

(Fan et al., 2005)

Lethality and fertility phenotypes are rescued by egh^+t7 and egh^hs.PG.

(Goode et al., 1996)

Lethality is rescued by egh^+t7, rescued adults are fertile.

(Williams et al., 1995)

Images (0)

Mutant

Wild-type

Stocks (4)

Bloomington

3902

egh⁷/FM7a/Dp(1;2;Y)w⁺