FB2024_03 , released June 25, 2024
Allele: Dmel\eghUAS.Tag:HA
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General Information
Symbol
Dmel\eghUAS.Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0194159
Feature type
allele
Associated gene
Dmel\egh
Associated Insertion(s)
Carried in Construct
P{UAS-egh.HA}
Key Links
Transgenic product class
wild_type
(Soller et al., 2006)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Soller et al., 2006)
Mutagen
in vitro construct
(Soller et al., 2006)
Progenitor genotype
Carried in construct
P{UAS-egh.HA}
Cytology
Description

UASt regulatory sequences drive expression of egh, that is tagged with Tag:HA at the C terminus. The construct has a ewg 3' UTR.

(Soller et al., 2006)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
egh
Tagged with
Tag:HA
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      eghUAS.Tag:HA/Scer\GAL4ap-md544 rescues eghEY03917

      (Ellis and Carney, 2011)

      Scer\GAL4elav-C155/eghUAS.Tag:HA rescues egh7/eghcm

      (Soller et al., 2006)

      eghUAS.Tag:HA/Scer\GAL4Tub.PU rescues egh7

      (Soller et al., 2006)

      Scer\GAL4elav-C155/eghUAS.Tag:HA rescues egh7

      (Soller et al., 2006)
      Partially rescues

      eghUAS.Tag:HA/Scer\GAL4Tub.PU partially rescues egh7/eghcm

      (Soller et al., 2006)

      eghUAS.Tag:HA/Scer\GAL4ap-md544 partially rescues egh7/eghcm

      (Soller et al., 2006)

      Scer\GAL4tsh-md621/eghUAS.Tag:HA partially rescues egh7/eghcm

      (Soller et al., 2006)

      Scer\GAL4tsh-md621/eghUAS.Tag:HA partially rescues egh7

      (Soller et al., 2006)
      Fails to rescue

      eghUAS.Tag:HA/Scer\GAL4gcm-rA87.C fails to rescue egh7/eghcm

      (Soller et al., 2006)

      eghUAS.Tag:HA/Scer\GAL4repo fails to rescue egh7/eghcm

      (Soller et al., 2006)

      eghUAS.Tag:HA/Scer\GAL4elav.PLu fails to rescue egh7/eghcm

      (Soller et al., 2006)

      eghUAS.Tag:HA/Scer\GAL4gcm-rA87.C fails to rescue egh7

      (Soller et al., 2006)

      eghUAS.Tag:HA/Scer\GAL4repo fails to rescue egh7

      (Soller et al., 2006)

      eghUAS.Tag:HA/Scer\GAL4elav.PLu fails to rescue egh7

      (Soller et al., 2006)

      eghUAS.Tag:HA/Scer\GAL4ap-md544 fails to rescue egh7

      (Soller et al., 2006)
      Comments

      The reduced courtship index of eghEY03917 males towards wild-type virgin females is rescued by expression of eghScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4ap-md544.

      (Ellis and Carney, 2011)

      Expression of eghScer\UAS.T:Ivir\HA1 under the control of either Scer\GAL4ap-md544 or Scer\GAL4tsh-md621 rescues the egg retention phenotype of eghcm/egh7 females. Most eghcm/egh7 larval brains show a complete rescue of the ventral nerve cord defect when eghScer\UAS.T:Ivir\HA1 in expressed under the control of Scer\GAL4ap-md544.

      Expression of eghScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4tsh-md621 rescues the viability of egh7 flies but these flies are short lived. Scer\GAL4ap-md544>eghScer\UAS.T:Ivir\HA1 expression does not rescue egh7 viability.

      Expression of eghScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4tsh-md621 rescues the receptivity response of eghcm/egh7 females to sex peptide completely. This response is partially rescued when eghScer\UAS.T:Ivir\HA1 is expressed under the control of either Scer\GAL4tub or Scer\GAL4ap-md544, but is not rescued when Scer\GAL4elav.PLu is used to drive expression. Oviposition after treatment with sex peptide is rescued in 67% of eghcm/egh7 females (the other 33% lay no eggs) when eghScer\UAS.T:Ivir\HA1 is expressed under the control of Scer\GAL4ap-md544 and is rescued to wild-type levels when driven by either Scer\GAL4tub or Scer\GAL4tsh-md621. Expression of eghScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4elav.PLu does not rescue oviposition.

      (Soller et al., 2006)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      eghScer\UAS.T:Ivir\HA1
      eghUAS.Tag:HA
      Name Synonyms
      Secondary FlyBase IDs
        References (2)