FlyBase Allele Report: Dmel\robo1[RNAi.UAS]

General Information

Symbol

Dmel\robo1^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0178178

Feature type

allele

Associated gene

Dmel\robo1

Associated Insertion(s)

Carried in Construct

P{UAS-robo1.RNAi}

Also Known As

UAS-RoboRNAi, UAS-robo-RNAi

Key Links

Transgenic product class

RNAi_reagent

(Tayler et al., 2004)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Tayler et al., 2004)

Mutagen

in vitro construct

(Tayler et al., 2004)

Progenitor genotype

Carried in construct

P{UAS-robo1.RNAi}

Cytology

Description

UASt regulatory sequences drive expression of a robo1 RNAi construct.

(Tayler et al., 2004)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

robo1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell migration | embryonic stage, with Scer\GAL4^Mef2.247

(Raza and Jacobs, 2016)

abnormal neuroanatomy | adult stage | female, with Scer\GAL4^Poxn.14

(Mellert et al., 2010)

abnormal neuroanatomy | adult stage | male, with Scer\GAL4^Poxn.14

(Mellert et al., 2010)

Phenotype Manifest In

embryonic heart cardioblast, with Scer\GAL4^Mef2.247

(Raza and Jacobs, 2016)

filopodium | embryonic stage, with Scer\GAL4^Mef2.247

(Raza and Jacobs, 2016)

lamellipodium | embryonic stage, with Scer\GAL4^Mef2.247

(Raza and Jacobs, 2016)

prothoracic leg taste bristle chemosensory neuron | female, with Scer\GAL4^Poxn.14

(Mellert et al., 2010)

prothoracic leg taste bristle chemosensory neuron | male, with Scer\GAL4^Poxn.14

(Mellert et al., 2010)

Detailed Description

Statement

Reference

The expression of robo1^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Pdf.PU does not significantly affect the length of sLNv axons compared to controls.

(Oliva et al., 2016)

The expression of robo1^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Mef2.247 leads to significant decreases in migration velocity, as well as in filopodial and lamellopodial extensions in embryonic heart cardioblasts, as compared to controls.

(Raza and Jacobs, 2016)

Males expressing robo^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Poxn.14 show an increase in midline crossing by foreleg gustatory receptor neuron (GRN) axons, with an increase in GRN axonal projections in the commissural region. Females expressing robo^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Poxn.14 often have visible crossing of the midline by foreleg GRN axons (this midline crossing is normally only seen in wild-type males, and not in wild-type females).

(Mellert et al., 2010)

Expression of a single copy of robo^{dsRNA.Scer\UAS} under the control of Scer\GAL4^elav-C155 or Scer\GAL4^sca-375.1 has no effect on visual system development.

(Tayler et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

Scer\GAL4^elav-C155/robo1^RNAi.UAS is a non-enhancer of abnormal neuroanatomy phenotype of egh⁷

(Fan et al., 2005)

NOT Suppressor of

Statement

Reference

Scer\GAL4^elav-C155/robo1^RNAi.UAS is a non-suppressor of abnormal neuroanatomy phenotype of egh⁷

(Fan et al., 2005)

Other

Statement

Reference

Scer\GAL4^αTub84B.PL, robo1^RNAi.UAS, robo2^RNAi.UAS, robo3^RNAi.UAS has abnormal neuroanatomy phenotype

(Tayler et al., 2004)

Scer\GAL4^sca-375.1, robo1^RNAi.UAS, robo3^RNAi.UAS has abnormal neuroanatomy phenotype

(Tayler et al., 2004)

Scer\GAL4^sca-375.1, robo1^RNAi.UAS, robo2^RNAi.UAS, robo3^RNAi.UAS has abnormal neuroanatomy phenotype

(Tayler et al., 2004)

Scer\GAL4^elav-C155, robo1^RNAi.UAS, robo2^RNAi.UAS, robo3^RNAi.UAS has abnormal neuroanatomy phenotype

(Tayler et al., 2004)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

Scer\GAL4^elav-C155/robo1^RNAi.UAS is a non-enhancer of photoreceptor cell R1 & axon phenotype of egh⁷

(Fan et al., 2005)

Scer\GAL4^elav-C155/robo1^RNAi.UAS is a non-enhancer of photoreceptor cell R2 & axon phenotype of egh⁷

(Fan et al., 2005)

Scer\GAL4^elav-C155/robo1^RNAi.UAS is a non-enhancer of photoreceptor cell R3 & axon phenotype of egh⁷

(Fan et al., 2005)

Scer\GAL4^elav-C155/robo1^RNAi.UAS is a non-enhancer of photoreceptor cell R4 & axon phenotype of egh⁷

(Fan et al., 2005)

Scer\GAL4^elav-C155/robo1^RNAi.UAS is a non-enhancer of photoreceptor cell R5 & axon phenotype of egh⁷

(Fan et al., 2005)

Scer\GAL4^elav-C155/robo1^RNAi.UAS is a non-enhancer of photoreceptor cell R6 & axon phenotype of egh⁷

(Fan et al., 2005)

NOT Suppressor of

Statement

Reference

Scer\GAL4^elav-C155/robo1^RNAi.UAS is a non-suppressor of photoreceptor cell R1 & axon phenotype of egh⁷

(Fan et al., 2005)

Scer\GAL4^elav-C155/robo1^RNAi.UAS is a non-suppressor of photoreceptor cell R2 & axon phenotype of egh⁷

(Fan et al., 2005)

Scer\GAL4^elav-C155/robo1^RNAi.UAS is a non-suppressor of photoreceptor cell R3 & axon phenotype of egh⁷

(Fan et al., 2005)

Scer\GAL4^elav-C155/robo1^RNAi.UAS is a non-suppressor of photoreceptor cell R4 & axon phenotype of egh⁷

(Fan et al., 2005)

Scer\GAL4^elav-C155/robo1^RNAi.UAS is a non-suppressor of photoreceptor cell R5 & axon phenotype of egh⁷

(Fan et al., 2005)

Scer\GAL4^elav-C155/robo1^RNAi.UAS is a non-suppressor of photoreceptor cell R6 & axon phenotype of egh⁷

(Fan et al., 2005)

Other

Statement

Reference

Scer\GAL4^Poxn.14, robo1^RNAi.UAS, robo2^RNAi.UAS, robo3^RNAi.UAS has prothoracic leg taste bristle chemosensory neuron | male phenotype

(Mellert et al., 2010)

Scer\GAL4^αTub84B.PL, robo1^RNAi.UAS, robo2^RNAi.UAS, robo3^RNAi.UAS has eye photoreceptor cell & axon phenotype

(Tayler et al., 2004)

Scer\GAL4^sca-375.1, robo1^RNAi.UAS, robo2^RNAi.UAS, robo3^RNAi.UAS has lamina anlage phenotype

(Tayler et al., 2004)

Scer\GAL4^sca-375.1, robo1^RNAi.UAS, robo2^RNAi.UAS, robo3^RNAi.UAS has eye photoreceptor cell & axon phenotype

(Tayler et al., 2004)

Scer\GAL4^elav-C155, robo1^RNAi.UAS, robo2^RNAi.UAS, robo3^RNAi.UAS has lamina anlage phenotype

(Tayler et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

The co-expression of either robo1^{dsRNA.Scer\UAS} and robo2^{dsRNA.Scer\UAS} or robo1^{dsRNA.Scer\UAS} and robo3^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Pdf.PU does not significantly affect the length of sLNv axons compared to controls.

(Oliva et al., 2016)

Males and females simultaneously co-expressing robo^{dsRNA.Scer\UAS}, lea^{dsRNA.Scer\UAS} and robo3^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Poxn.14 show severe disruption of foreleg gustatory receptor neuron axon morphology, with axons collapsing onto the midline.

(Mellert et al., 2010)

Expression of robo^{dsRNA.Scer\UAS}, under the control of Scer\GAL4^elav-C155, does not alter the R-cell phenotype of egh⁷ mutants.

(Fan et al., 2005)

Simultaneous co-expression of robo^{dsRNA.Scer\UAS}, lea^{dsRNA.Scer\UAS} and robo3^{dsRNA.Scer\UAS} under the control of Scer\GAL4^elav-C155 results in defects in visual system development; distal cell neurons invade the developing lamina. Simultaneous co-expression of robo^{dsRNA.Scer\UAS}, lea^{dsRNA.Scer\UAS} and robo3^{dsRNA.Scer\UAS} under the control of Scer\GAL4^sca-375.1 results in distal cell neurons invading the developing lamina. The distal cell neurons and lamina glia are intermingled in these animals. In addition defects in photoreceptor axon targeting are seen, with the regions of mistargeting correlating with the regions where the distal cell neurons enter the lamina. Co-expression of two copies of robo^{dsRNA.Scer\UAS} and two copies of robo3^{dsRNA.Scer\UAS} under the control of Scer\GAL4^sca-375.1 results in modest defects in the visual system. Simultaneous co-expression of robo^{dsRNA.Scer\UAS}, lea^{dsRNA.Scer\UAS} and robo3^{dsRNA.Scer\UAS} under the control of Scer\GAL4^αTub84B.PL results in many photoreceptor axons extending through the lamina and too many photoreceptor axons entering the medulla. Simultaneous co-expression of robo^{dsRNA.Scer\UAS}, lea^{dsRNA.Scer\UAS} and robo3^{dsRNA.Scer\UAS} under the control of Scer\GAL4^GMR.PF does not result in any defects in photoreceptor axon targeting.

(Tayler et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

9285

w^*; P{UAS-robo1.RNAi}3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

robo1^RNAi.UAS

robo1^{dsRNA.Scer\UAS}

robo1^dsRNA.UAS

robo^{dsRNA.Scer\UAS}

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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