FB2024_03 , released June 25, 2024
Allele: Dmel\robo2UAS.EGFP
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General Information
Symbol
Dmel\robo2UAS.EGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0284777
Feature type
allele
Associated gene
Dmel\robo2
Associated Insertion(s)
Carried in Construct
P{UAS-robo2.EGFP}
Also Known As
UAS-ROBO2-EGFP
Key Links
Transgenic product class
cDNA
(Katsuki et al., 2009)
wild_type
(Katsuki et al., 2009)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Katsuki et al., 2009)
wild_type
(Katsuki et al., 2009)
Mutagen
in vitro construct
(Katsuki et al., 2009)
Progenitor genotype
Carried in construct
P{UAS-robo2.EGFP}
Cytology
Description

UAS drives expression of a robo2 cDNA (minus its 3' UTR) with a C-terminal EGFP tag.

(Katsuki et al., 2009)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
robo2
Tagged with
EGFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The expression of robo2UAS.EGFP under the control of Scer\GAL4Pdf.PU results in adult sLNv neurons exhibiting significantly shorter axon projections and failing to reach their targets, but does not have an obvious effect on the lLNv neurons, as compared to controls; this defect occurs in the early pupa.

      The expression of robo2UAS.EGFP under the control of Scer\GAL4ato.PU results in adult DCN neurons failing to form the characteristic commissure and instead stalling in the proximity of the mushroom body peduncle in the larval brain, eventually turning around and innervating the ipsilateral optic lobes in adult brains.

      (Oliva et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The expression of robo2UAS.EGFP leads to shorter sLNv axon projections (Scer\GAL4Pdf.PU-driven expression) and to the absence of the normal DCN neuron commissure (Scer\GAL4ato.PU-driven expression), both of which are suppressed by sli2/slik04807b transheterozygosity. The shorter sLNv axon projections resulting from the Scer\GAL4Pdf.PU-driven expression are also partially suppressed by the co-expression of Ptp69DKK105220.

      The DCN axon projection defects resulting from the expression of robo2UAS.EGFP under the control of Scer\GAL4ato.PU are enhanced by the co-expression of Ptp69DUAS.cDa.Tag:HA.

      (Oliva et al., 2016)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      leaScer\UAS.T:Avic\GFP-EGFP
      robo2Scer\UAS.T:Avic\GFP-EGFP
      robo2UAS.EGFP
      Name Synonyms
      Secondary FlyBase IDs
        References (2)