FB2024_03 , released June 25, 2024
Allele: Dmel\sliE-158
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General Information
Symbol
Dmel\sliE-158
Species
D. melanogaster
Name
FlyBase ID
FBal0035623
Feature type
allele
Associated gene
Dmel\sli
Associated Insertion(s)
P{lArB}sliE-158
Carried in Construct
Also Known As
slitE158, sliE158, slitE-158
Key Links
Genomic Maps

Allele class

loss of function allele

hypomorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

hypomorphic allele - genetic evidence

(Battye et al., 1999)

loss of function allele

(Battye et al., 2001)
Mutagen
P-element activity
(Nambu et al., 1990, Rothberg et al., 1990)
Progenitor genotype
Associated Insertion(s)
P{lArB}sliE-158
Cytology
Description

P-element insert 500bp 5' to the start of translation.

(Nambu et al., 1990)

P{lArB} insertion 105bp upstream of the coding region and transcription initiation sequence.

(Rothberg et al., 1990)
Allele components
Component
Use(s)
Inserted element
P{lArB}
Encoded product / tool
lacZ
Tagged with
Tag:NLS(P)
Mutations Mapped to the Genome
Curation Data
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      sliE-158 homozygous embryos exhibit severe axon guidance defects in the longitudinal connective from 14h post-fertilization onwards, as compared to controls.

      (Manavalan et al., 2017)

      Medial longitudinal tracts are seen to cross the midline in 14 hour mutant embryos.

      (Bhat, 2005)

      slik04807b/sliE-158 third instar larvae show defects in photoreceptor axon targeting, with gaps in the lamina and increased numbers of axons entering the medulla.

      (Tayler et al., 2004)

      3.1% of expected mutant embryos hatch as first instar larvae. The latest observed stage of mutants is pupa. Nerve cord length is 82% of embryo length. Midline guidance failure of repellence phenotype, evidenced by midline crossing, is mild.

      (Battye et al., 2001)

      Embryos show a partial midline collapse of the central nervous system.

      (Kidd et al., 1999)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Enhanced by
      Statement
      Reference

      sliE-158 has abnormal neuroanatomy phenotype, non-enhanceable by fraUAS.cKa/Scer\GAL4elav.PLu

      (Bhat, 2005)
      Phenotype Manifest In
      Enhanced by
      Statement
      Reference

      sliE-158 has larval ventral nerve cord phenotype, enhanceable by robo[+]/robo11

      (Battye et al., 2001)

      sliE-158 has larval longitudinal connective phenotype, enhanceable by robo[+]/robo11

      (Battye et al., 1999)
      NOT Enhanced by
      Statement
      Reference

      sliE-158 has larval longitudinal connective phenotype, non-enhanceable by fraUAS.cKa/Scer\GAL4elav.PLu

      (Bhat, 2005)
      Enhancer of
      Statement
      Reference

      sli[+]/sliE-158 is an enhancer of larval longitudinal connective phenotype of robo11

      (Battye et al., 1999)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The longitudinal tracts of embryos expressing NetBScer\UAS.cHa under the control of Scer\GAL4unspecified in a sliE-158 background appear similar to those seen in strong loss-of-function sli mutant embryos. Expression of fraScer\UAS.cKa under the control of Scer\GAL4elav.PLu does not enhance the sliE-158 longitudinal tract phenotype.

      (Bhat, 2005)

      Percentage of segments with midline crossovers in robo1/sliE-158 transheterozygotes is 35%.

      (Battye et al., 2001)

      Embryos heterozygous for both robo1 and sliE-158 show deviation of longitudinal fascicles towards the midline in 32% of segments.

      (Battye et al., 1999)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (8)
      Reported As
      Symbol Synonym
      E158
      (Rothberg et al., 1990)
      sli158
      (Battye et al., 1999)
      sliE-158
      sliE158
      (Bhat, 2005, Tayler et al., 2004)
      slitE-158
      (Manavalan et al., 2017, Kidd et al., 1999)
      slitE158
      (Parsons et al., 2003, Battye et al., 2001)
      slite158
      (Chance and Bashaw, 2015)
      slithypo
      (Manavalan et al., 2017)
      Name Synonyms
      Secondary FlyBase IDs
        References (10)