FB2024_03 , released June 25, 2024
Allele: Dmel\TfAP-215
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General Information
Symbol
Dmel\TfAP-215
Species
D. melanogaster
Name
FlyBase ID
FBal0123337
Feature type
allele
Associated gene
Dmel\TfAP-2
Associated Insertion(s)
Carried in Construct
Also Known As
dAP-215, AP-215
Key Links
Genomic Maps

Allele class

amorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - genetic evidence

(Monge et al., 2001)
Mutagen
ethyl methanesulfonate
(Kerber et al., 2001, Monge et al., 2001)
Progenitor genotype
Cytology
Description

Nucleotide substitution: GT changed to GA in exon 7 splice donor.

(Monge et al., 2001)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3L:21,612,782..21,612,782 [+]
Nucleotide change:

T21612782A

Reported nucleotide change:

T?A

Comment:

Reported as nucleotide substitution: GT to GA in the exon 7 splice donor.

(Monge et al., 2001)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of AP-2Scer\UAS.cMa under the control of Scer\GAL4AP-2.E6 partially rescues the AP-22/AP-215 leg phenotypes. In some cases, an almost complete rescue of the outgrowth defect in the proximal and intermediate leg segments (coxa, trochanter, femur and tibia) is observed. In the distal leg, Scer\GAL4AP-2.E6-driven AP-2Scer\UAS.cMa significantly rescues the outgrowth defect of the tarsus, but fails to restore tarsal joints.

      (Ahn et al., 2011)

      AP-22/AP-215 mutant males display a reduced accessory gland size, vas deferens are narrower at their distal ends, and the anterior ejaculatory ducts less bulbous.

      (Chatterjee et al., 2011)

      In AP-215 homozygotes, all leg joints fail to form and the leg is shortened along the proximal-distal axis. The femur and tibia are reduced in length, and in the distal leg only a fusion of tarsal segments 1 and 5 remains.

      Legs from AP-21215/AP-215 flies show a very similar phenotype to AP-21215 homozygous mutants.

      (Ciechanska et al., 2007)

      Heterozygotes with AP-2stummelbein show a similar mutant short leg/no joint phenotype as do AP-2stummelbein homozygotes. Early stages of axial patterning of the leg disc appear normal. Somatic clones induced in the interjoint region do not show any mutant phenotype. When the clones cross between tarsal segments the joint is affected.

      (Kerber et al., 2001)

      Pharate pupae and the few eclosing adults show a short leg phenotype. Leg length = 30% wild type. Tarsal joints are lacking. Legs are non-functional. The proximodistal order of segments is not grossly affected as landmarks for the coxa, femur, tibia, and first and last (5th) tarsal segments (sex comb and claws, respectively) are present in the correct order. Rows of misoriented sensory bristles indicate that proximodistal polarity is locally perturbed. The stunted legs show no nervous activity except for a faint twitching of the claws and of cuticle over the femur-tibia junction. The proboscis is reduced in length and width. Null mutants show a substantial reduction in length and width of the labellum and number of pseudo tracheal rows. The prestomal cavity is present and newly eclosed adults can drink. Clypeus, maxillary palps and antennae are apparently normal but there is increased spacing between antennae.

      (Monge et al., 2001)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to complement

      TfAP-2stummelbein

      (Kerber et al., 2001)
      Partially rescued by

      TfAP-215/TfAP-22 is partially rescued by TfAP-2UAS.cMa/Scer\GAL4AP-2.E6

      (Ahn et al., 2011)
      Comments

      Expression of AP-2Scer\UAS.cMa under the control of Scer\GAL4AP-2.E6 partially rescues the AP-22/AP-215 leg phenotypes. In some cases, an almost complete rescue of the outgrowth defect in the proximal and intermediate leg segments (coxa, trochanter, femur and tibia) is observed. In the distal leg, Scer\GAL4AP-2.E6-driven AP-2Scer\UAS.cMa significantly rescues the outgrowth defect of the tarsus, but fails to restore tarsal joints.

      (Ahn et al., 2011)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Alleles fall into an allelic series based on leg length defect. In order of decreasing severity of phenotype: AP-22 = AP-23 = AP-24 = AP-25 = AP-213 = AP-214 = AP-215 = AP-216 = AP-219 > AP-28 = AP-212 > AP-211 > AP-217 > AP-218 > AP-210 = AP-29.

      (Monge et al., 2001)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      15
      (Ahn et al., 2011)
      AP-215
      (Pinto et al., 2022, Chatterjee et al., 2011)
      TfAP-215
      (Kucherenko et al., 2016)
      dAP-215
      (Guarner et al., 2014, Ciechanska et al., 2007, Kerber et al., 2001)
      Name Synonyms
      Secondary FlyBase IDs
        References (8)