FlyBase Allele Report: Dmel\TfAP-2[UAS.cMa]

General Information

Symbol

Dmel\TfAP-2^UAS.cMa

Species

D. melanogaster

Name

FlyBase ID

FBal0123326

Feature type

allele

Associated gene

Dmel\TfAP-2

Associated Insertion(s)

Carried in Construct

P{UAS-AP-2.M}

Also Known As

UAS-dAP-2

Key Links

Transgenic product class

cDNA

(Monge et al., 2001)

wild_type

(Monge et al., 2001)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Monge et al., 2001)

wild_type

(Monge et al., 2001)

Mutagen

in vitro construct

(Kerber et al., 2001, Monge et al., 2001)

Progenitor genotype

Carried in construct

P{UAS-AP-2.M}

Cytology

Description

Expression of the A isoform of TfAP-2 is under the control of UASt.

(Mitchell, 2007.4)

Expression of TfAP-2 cDNA is governed by UASt regulatory sequences.

(Monge et al., 2001)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

TfAP-2

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased rate of movement, with Scer\GAL4^p339

(Monge et al., 2001)

lethal - all die before end of larval stage, with Scer\GAL4^αTub84B.PB

(Monge et al., 2001)

some die during larval stage, with Scer\GAL4^αTub84B.PB

(Monge et al., 2001)

visible, with Scer\GAL4^AP-2.E6, TfAP-2¹⁵/TfAP-2²

(Ahn et al., 2011)

visible, with Scer\GAL4^p339

(Monge et al., 2001)

Phenotype Manifest In

labellum, with Scer\GAL4^p339

(Monge et al., 2001)

leg | somatic clone, with Scer\GAL4^αTub84B.PB

(Monge et al., 2001)

leg joint | ectopic, with Scer\GAL4^ptc-559.1

(Kerber et al., 2001)

leg joint | increased number, with Scer\GAL4^ptc-559.1

(Kerber et al., 2001)

leg joint | somatic clone, with Scer\GAL4^αTub84B.PB

(Monge et al., 2001)

leg joint | somatic clone | increased number, with Scer\GAL4^αTub84B.PB

(Monge et al., 2001)

macrochaeta, with Scer\GAL4^p339

(Monge et al., 2001)

tarsal segment, with Scer\GAL4^AP-2.E6, TfAP-2¹⁵/TfAP-2²

(Ahn et al., 2011)

wing, with Scer\GAL4^p339

(Monge et al., 2001)

wing & sense organ | ectopic, with Scer\GAL4^p339

(Monge et al., 2001)

wing | somatic clone, with Scer\GAL4^αTub84B.PB

(Monge et al., 2001)

wing vein L3, with Scer\GAL4^p339

(Monge et al., 2001)

wing vein L4, with Scer\GAL4^p339

(Monge et al., 2001)

Detailed Description

Statement

Reference

The expression of TfAP-2^Scer\UAS.cMa under the control of Scer\GAL4^elav.PU, either throughout development and adulthood, or only during adulthood (temporally regulated by Gal80[ts]) does not lead to any significant change in sleep time, as compared to controls.

(Kucherenko et al., 2016)

Expression of AP-2^Scer\UAS.cMa under the control of Scer\GAL4^AP-2.E6 partially rescues the AP-2²/AP-2¹⁵ leg phenotypes. In some cases, an almost complete rescue of the outgrowth defect in the proximal and intermediate leg segments (coxa, trochanter, femur and tibia) is observed. In the distal leg, Scer\GAL4^AP-2.E6-driven AP-2^Scer\UAS.cMa significantly rescues the outgrowth defect of the tarsus, but fails to restore tarsal joints.

(Ahn et al., 2011)

When expression is driven by Scer\GAL4^ptc-559.1 many ectopic joints, with wild type morphology, appear in the tarsal segments. The ectopic joints are clustered together.

(Kerber et al., 2001)

When expression is driven by Scer\GAL4^αTub84B.PB between 0-24hr of development larval lethality occurs. Activation between 24-48 hours (first and second instar larvae) results in increased survival of adults with leg and wing defects of varying severity. Leg defects include shortening, and necrotic, abnormal joints in addition to the induction of supernumerary joints. When expression is driven by Scer\GAL4^p339 adults are ataxic, have abnormal labellums, misoriented notal bristles, wings with non-adherent dorsal and ventral surfaces and two rows of ectopic sensory bristles on both the dorsal and ventral and surfaces of the wing blade which apparently result from transformations of the distal portion of wing veins L3 and L4.

(Monge et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^ptc-559.1, TfAP-2^UAS.cMa has leg joint | increased number phenotype, suppressible by N^l1N-ts1

(Kerber et al., 2001)