FB2024_03 , released June 25, 2024
Allele: Dmel\hbUAS.cWa
Open Close
General Information
Symbol
Dmel\hbUAS.cWa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Wimmer
FlyBase ID
FBal0104606
Feature type
allele
Associated gene
Dmel\hb
Associated Insertion(s)
Carried in Construct
P{UAS-hb.W}
Also Known As
UAS-hb, UAS-hbF4A
Key Links
Transgenic product class
wild_type
(Wimmer et al., 2000)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Wimmer et al., 2000)
Mutagen
in vitro construct
(Janody et al., 2000)
Progenitor genotype
Carried in construct
P{UAS-hb.W}
Cytology
Description

UASt regulatory sequences are fused upstream of a 2.4kb fragment containing the hb open reading frame.

(Wimmer et al., 2000)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
hb
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class

      abnormal cell number | larval stage, with Scer\GAL4eg-Mz360

      (Pérez et al., 2022, Guntur et al., 2021)

      abnormal cell number | larval stage, with Scer\GAL4elav-C155

      (Pérez et al., 2022)

      abnormal neuroanatomy

      (Losada-Pérez et al., 2010)

      abnormal neuroanatomy, with Scer\GAL4en-e16E

      (Tran et al., 2010, Pearson and Doe, 2003)

      abnormal neuroanatomy, with Scer\GAL4en-e16E, hb15/hbP1only

      (Tran et al., 2010)

      abnormal neuroanatomy, with Scer\GAL4pros.PMG

      (Tran et al., 2010)

      abnormal neuroanatomy | embryonic stage, with Scer\GAL4en-e16E

      (Kohwi et al., 2013)

      abnormal neuroanatomy | first instar larval stage, with Hsim\VP16AD.ac, Scer\GAL4DBD.gsb, Scer\GAL4KZip+.R25A05

      (Seroka and Doe, 2019)

      abnormal neuroanatomy | heat sensitive, with Scer\GAL4pros.PMG

      (Pearson and Doe, 2003)

      increased cell number | embryonic stage, with Scer\GAL4ase.PZ

      (Fox et al., 2022)

      increased cell number | embryonic stage, with Scer\GAL4eg-Mz360

      (Pérez et al., 2022)

      increased cell number | embryonic stage, with Scer\GAL4hkb.PU

      (Pérez et al., 2022)

      increased cell number | first instar larval stage, with Hsim\VP16AD.ac, Scer\GAL4DBD.gsb, Scer\GAL4KZip+.R25A05

      (Seroka and Doe, 2019)

      increased cell number | larval stage, with Scer\GAL4hkb.PU

      (Pérez et al., 2022)

      lethal - all die before end of larval stage, with Scer\GAL4eg-Mz360

      (Guntur et al., 2021)

      lethal - all die before end of pupal stage, with Scer\GAL4hkb.PU

      (Pérez et al., 2022)

      lethal - all die during embryonic stage | recessive, with Scer\GAL4GCN4.nanos.bcd3'UTR

      (Janody et al., 2000)

      some die during embryonic stage | recessive, with Scer\GAL4GCN4.nanos.bcd3'UTR

      (Janody et al., 2000)
      Phenotype Manifest In

      cephalopharyngeal skeleton, with Scer\GAL4GCN4.nanos.bcd3'UTR

      (Janody et al., 2000)

      dorsal arm of pharyngeal sclerite, with Scer\GAL4GCN4.nanos.bcd3'UTR

      (Janody et al., 2000)

      dorsal bridge, with Scer\GAL4GCN4.nanos.bcd3'UTR

      (Janody et al., 2000)

      embryonic/larval esophagus | embryonic stage, with Scer\GAL4GCN4.nanos.bcd3'UTR

      (Janody et al., 2000)

      embryonic/larval neuromuscular junction | first instar larval stage, with Hsim\VP16AD.ac, Scer\GAL4DBD.gsb, Scer\GAL4KZip+.R25A05

      (Seroka and Doe, 2019)

      embryonic/larval pharynx | embryonic stage, with Scer\GAL4GCN4.nanos.bcd3'UTR

      (Janody et al., 2000)

      embryonic/larval proventriculus | embryonic stage, with Scer\GAL4GCN4.nanos.bcd3'UTR

      (Janody et al., 2000)

      embryonic/larval tracheal dorsal trunk, with Scer\GAL412.1

      (Wolf and Schuh, 2000)

      embryonic ventral nerve cord, with Scer\GAL4eg-Mz360

      (Pérez et al., 2022)

      embryonic ventral nerve cord, with Scer\GAL4hkb.PU

      (Pérez et al., 2022)

      epipharyngeal sclerite, with Scer\GAL4GCN4.nanos.bcd3'UTR

      (Janody et al., 2000)

      EW1 neuron | ectopic, with Scer\GAL4en-e16E

      (Isshiki et al., 2001)

      eye, with Scer\GAL4GMR.PF

      (Goto et al., 2011)

      FMRFamide SE2 neuron | increased number, with Scer\GAL4elav-C155

      (Losada-Pérez et al., 2010)

      ganglion mother cell GMC1-1a | ectopic, with Scer\GAL4en-e16E

      (Isshiki et al., 2001)

      gastric caecum, with Scer\GAL4GCN4.nanos.bcd3'UTR

      (Janody et al., 2000)

      larval DA1 motor neuron | ectopic, with Scer\GAL4en-e16E

      (Isshiki et al., 2001)

      larval DA2 motor neuron, with Scer\GAL4sca-109-68

      (Grosskortenhaus et al., 2005)

      larval DA3 motor neuron, with Scer\GAL4sca-109-68

      (Grosskortenhaus et al., 2005)

      larval DO1 motor neuron | ectopic, with Scer\GAL4en-e16E

      (Pearson and Doe, 2003)

      larval DO1 motor neuron | ectopic | embryonic stage, with Scer\GAL4en-e16E

      (Kohwi et al., 2013)

      larval DO1 motor neuron | ectopic | heat sensitive, with Scer\GAL4pros.PMG

      (Pearson and Doe, 2003)

      larval DO2 motor neuron

      (Grosskortenhaus et al., 2005)

      larval DO2 motor neuron | ectopic | embryonic stage, with Scer\GAL4en-e16E

      (Kohwi et al., 2013)

      larval DO2 motor neuron | ectopic | heat sensitive, with Scer\GAL4pros.PMG

      (Pearson and Doe, 2003)

      larval fpCC neuron | ectopic, with Scer\GAL4en-e16E

      (Novotny et al., 2002)

      larval fpCC neuron | ectopic, with Scer\GAL4sca.PC

      (Novotny et al., 2002)

      larval LL1 motor neuron, with Scer\GAL4sca-109-68

      (Grosskortenhaus et al., 2005)

      larval RP2 motor neuron | ectopic, with Scer\GAL4en-e16E

      (Isshiki et al., 2001)

      larval ventral nerve cord, with Scer\GAL4eg-Mz360

      (Pérez et al., 2022, Guntur et al., 2021)

      larval ventral nerve cord, with Scer\GAL4elav-C155

      (Pérez et al., 2022)

      larval ventral nerve cord, with Scer\GAL4hkb.PU

      (Pérez et al., 2022)

      lineage NB7-1 primary motor neuron | first instar larval stage, with Hsim\VP16AD.ac, Scer\GAL4DBD.gsb, Scer\GAL4KZip+.R25A05

      (Seroka and Doe, 2019)

      medial-most cell body glial cell | ectopic, with Scer\GAL4en-e16E

      (Isshiki et al., 2001)

      median tooth, with Scer\GAL4GCN4.nanos.bcd3'UTR

      (Janody et al., 2000)

      muscle cell of dorsal oblique muscle 1 | first instar larval stage, with Hsim\VP16AD.ac, Scer\GAL4DBD.gsb, Scer\GAL4KZip+.R25A05

      (Seroka and Doe, 2019)

      muscle cell of dorsal oblique muscle 2 | first instar larval stage, with Hsim\VP16AD.ac, Scer\GAL4DBD.gsb, Scer\GAL4KZip+.R25A05

      (Seroka and Doe, 2019)

      muscle cell of lateral longitudinal muscle 1 | first instar larval stage, with Hsim\VP16AD.ac, Scer\GAL4DBD.gsb, Scer\GAL4KZip+.R25A05

      (Seroka and Doe, 2019)

      neuroblast NB7-3, with Scer\GAL4eg-Mz360

      (Novotny et al., 2002)

      pCC neuron | ectopic, with Scer\GAL4en-e16E

      (Isshiki et al., 2001)

      precursor cell of the stomatogastric nervous system, with Scer\GAL4GCN4.nanos.bcd3'UTR

      (Janody et al., 2000)

      U neuron | ectopic, with Scer\GAL4en-e16E

      (Novotny et al., 2002)

      U neuron | ectopic, with Scer\GAL4sca.PC

      (Novotny et al., 2002)

      U neuron | embryonic stage | increased number, with Scer\GAL4ase.PZ

      (Fox et al., 2022)

      U neuron | increased number, with Scer\GAL4en-e16E

      (Tran et al., 2010)

      U neuron | increased number, with Scer\GAL4en-e16E, hb15/hbP1only

      (Tran et al., 2010)

      U neuron | increased number, with Scer\GAL4pros.PMG

      (Tran et al., 2010)

      ventral arm of pharyngeal sclerite, with Scer\GAL4GCN4.nanos.bcd3'UTR

      (Janody et al., 2000)
      Detailed Description
      Statement
      Reference

      Expressing hbUAS.cWa under the control of the Split GAL4 pair Scer\GAL4DBD.gsb and Hsim\VP16AD.ac in combination with Scer\GAL4KZip+.R25A05 (the dominant-negative repressor of activity in the Split GAL4 system KZip+ expressed in the pattern of GMR25A05) induces changes in the morphology of late-born motor neurons developing from neuroblast NB7-1. Late-born motor neurons adopt a morphology similar to endogenous U1 neurons, producing a dorsal contralateral dendritic arbor which targets the same region of the neuropil as controls and an axon which exclusively innervates dorsal oblique body wall muscles DO1 and DO2. In hbUAS.cWa-expressing larvae, a significantly increased number of presynaptic brp-immunopositive puncta form on DO2 (but not DO1) at the expense of the lateral longitudinal muscle LL1 compared to controls. Unlike endogenous U1 neurons, in late-born hbUAS.cWa-expressing U motor neurons the dendritic arbor emerges from a dorsal location and not from the cell body. hbUAS.cWa overexpression leads to the generation of supernumerary U motor neurons with a shifted temporal identity, progressively adopting a typical 'late-born' morphology.

      U motor neurons in stage 13-15 embryos expressing hbUAS.cWa under the control of the Split GAL4 pair Scer\GAL4DBD.gsb and Hsim\VP16AD.ac in combination with Scer\GAL4KZip+.R25A05 display a sequential arbor extension and a gross arbor distribution similar to wild-type controls: early-born neurons project axons and dendrites before later-born neurons and have longer axon and dendritic projections. hbUAS.cWa overexpression has no effect on the morphology of the endogenous U1 or U2 neurons.

      (Seroka and Doe, 2019)

      Expression of hbScer\UAS.cWa under the control of Scer\GAL4en-e16E in embryos induces the U1/U2 fate, resulting in 20.3 +/- 5.1% of NB7-1 neuroblast lineages having more than five hb+ U1/U2 neurons in these animals.

      (Kohwi et al., 2013)

      Overexpression of hbScer\UAS.cWa in the developing eye under the control of Scer\GAL4GMR.PF generates a rough eye phenotype.

      (Goto et al., 2011)

      Expression of hbScer\UAS.cWa under the control of Scer\GAL4elav-C155 results in a significant increase in the number of SE2 neurons specified.

      (Losada-Pérez et al., 2010)

      In wild-type, neuroblast NB7-1 generates five U motoneurons. Overexpression of hbScer\UAS.cWa in hb15/hbP1only mutant NB7-1 under the control of Scer\GAL4en-e16E produces an average of twelve U neurons per hemisegment.

      Overexpression of hbScer\UAS.cWa in NB7-1 under the control of Scer\GAL4en-e16E produces an average of seventeen U neurons per hemisegment.

      Overexpression of hbScer\UAS.cWa in embryos under the control of Scer\GAL4pros.PMG generates an average of nine U neurons per hemisegment.

      (Tran et al., 2010)

      In hbScer\UAS.cWa ; Scer\GAL4sca-109-68 embryos motor neurons U2-U5 are born and differentiate much later than normal (first detected during stage 16 rather than during stage 13 as in wild-type). Their cell bodies are also mis-positioned.

      (Grosskortenhaus et al., 2005)

      Expression of two copies of hbScer\UAS.cWa under the control of Scer\GAL4pros.PMG at 23oC in the NB7-1 lineage just after the birth of the hb-negative GMC3 has no effect on the normal U1-U3 fates, but subsequently the neuroblast generates an average of 6.3 extra U1 motor neurons (based on molecular marker expression). Expression of one copy of hbScer\UAS.cWa under the control of Scer\GAL4pros.PMG at 18oC in the NB7-1 lineage just after the birth of the hb-negative GMC3 results in the production of ectopic presumptive U2 neurons. Expression of two copies of hbScer\UAS.cWa under the control of Scer\GAL4en-e16E results in a massive induction of U1 neurons. U neuron identity remains wild type in embryos expressing hbScer\UAS.cWa under the control of eve2xCQ.RC.T:Scer\GAL4.

      (Pearson and Doe, 2003)

      When hbScer\UAS.cWa is driven by Scer\GAL4eg-Mz360 mostly five to eight NB7-3 derived clls in abdominal segments compared to four in wild-type). However all of these neurons express markers of the early sublineage, while those of the late sublineage are missing. When hbScer\UAS.cWa is driven by Scer\GAL4sca.PC or Scer\GAL4en-e16E, an increase in eve positive neurons are seen in the CQ position, these are probably additional fpCCs and/or dorsal CQ neurons.

      (Novotny et al., 2002)

      When hbScer\UAS.cWa is driven by Scer\GAL4en-e16E, there are additional neurons in the EW1 neuron lineage indicating a duplication of the GMC1 fate. There also duplication of the first born aCC/pCC or duplications or triplications of RP2. Extra MM-CB glia are seen at the midline and there is a decrease in the number of midline channel glia.

      (Isshiki et al., 2001)

      Embryos expressing hbScer\UAS.cWa under the control of Scer\GAL4nos.bcd3'UTR.T:Scer\GCN4 (where Scer\GAL4nos.bcd3'UTR.T:Scer\GCN4 is provided maternally) show 80% embryonic lethality. The few surviving larvae never reach adulthood. 30% of the embryos show a strong head phenotype that mimics the tor loss-of-function phenotype; the median tooth and epistomal sclerite are absent, and the pharyngeal arms of the mouth skeleton, including the dorsal bridge, the dorsal arm and ventral arm are extremely reduced. The oesophagus is not apparent. 16% of embryos have a less severe phenotype, showing mostly truncations of a part of the dorsal bridge, distortions of the pharyngeal wall and defects of the labrum derivatives, which are either absent, truncated or displaced. Most remaining embryos (52%) have head involution defects that might be secondary. The fkh- and Kr-expressing cells of the stomatogastric nervous system precursor invaginations are missing. All surviving larvae derived from embryos expressing hbScer\UAS.cWa under the control of Scer\GAL4nos.bcd3'UTR.T:Scer\GCN4 (where Scer\GAL4nos.bcd3'UTR.T:Scer\GCN4 is provided maternally) have feeding problems, since food never reaches the midgut. Food accumulates in the pharynx and oesophagus, which is drastically shortened. The gastric caeca are slightly altered and food never reaches the proventriculus, which has an abnormal morphology.

      (Janody et al., 2000)

      Expression of hbScer\UAS.cWa driven by Scer\GAL412.1 (in sensory organ precursor cells in positions close to bridge cells) leads to the dorsal trunk of the embryo showing interruptions and abnormal bottleneck-like fusion points.

      (Wolf and Schuh, 2000)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhanced by
      Suppressed by
      Enhancer of
      Statement
      Reference

      hbUAS.cWa/Scer\GAL4GMR.PF is an enhancer of eye phenotype of Scer\GAL4GMR.PF, fruNP0021

      (Goto et al., 2011)
      NOT Enhancer of
      Suppressor of
      NOT Suppressor of
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The percentage of NB7-1 neuroblast lineages containing more than hb+ U1/U2 neurons is increased from 20.3 +/- 5.1% in animals expressing hbScer\UAS.cWa under the control of Scer\GAL4en-e16E to 50.3 +/- 3.6% in animals co-expressing LamTRiP.cUa and hbScer\UAS.cWa under the control of Scer\GAL4en-e16E.

      Co-expression of danScer\UAS.cEa in embryos expressing hbScer\UAS.cWa under the control of Scer\GAL4en-e16E increases the median number of U1/U2 neurons per NB7-1 neuroblast lineage from approximately five to approximately eight.

      (Kohwi et al., 2013)

      Simultaneous overexpression of hbScer\UAS.cWa and fruNP0021 in the developing eye under the control of Scer\GAL4GMR.PF disrupts the developing eye structure to a greater extent than expression of each transgene alone.

      (Goto et al., 2011)

      Co-expression of hbScer\UAS.cWa and casScer\UAS.cKa in neuroblast NB7-1 under the control of Scer\GAL4en-e16E produces an average of fifteen U neurons per hemisegment.

      Co-expression of hbScer\UAS.cWa and pdm2Scer\UAS.T:Ivir\HA1 in neuroblast NB7-1 under the control of Scer\GAL4en-e16E produces an average of nine U neurons per hemisegment.

      (Tran et al., 2010)

      The embryonic lethality seen in embryos expressing hbScer\UAS.cWa under the control of Scer\GAL4nos.bcd3'UTR.T:Scer\GCN4 (where Scer\GAL4nos.bcd3'UTR.T:Scer\GCN4 is provided maternally) is increased if they are derived from females carrying one copy of bcd6.

      (Janody et al., 2000)

      Embryos derived from homozygous bcd6 mothers carrying four copies of Scer\GAL4nos.bcd3'UTR.T:Scer\GCN4 and fathers carrying hbScer\UAS.cWa homozygously show suppression of anterior tail structures and thoracic structures are rescued to variable extent, up to a complete rescue of T2 and T3.

      (Wimmer et al., 2000)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer

      E. Wimmer

      (Janody et al., 2000)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      hbScer\UAS.cWa
      hbUAS.cWa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Wimmer
      (Janody et al., 2000)
      Secondary FlyBase IDs
        References (20)