FlyBase Allele Report: Dmel\cas[UAS.cKa]

General Information

Symbol

Dmel\cas^UAS.cKa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Kambadur

FlyBase ID

FBal0086694

Feature type

allele

Associated gene

Dmel\cas

Associated Insertion(s)

Carried in Construct

P{UAS-cas.K}

Also Known As

UAS-cas

Key Links

Transgenic product class

cDNA

(Kambadur et al., 1998)

wild_type

(Kambadur et al., 1998)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Kambadur et al., 1998)

wild_type

(Kambadur et al., 1998)

Mutagen

in vitro construct

(Kambadur et al., 1998)

Progenitor genotype

Carried in construct

P{UAS-cas.K}

Cytology

Description

UASt regulatory sequences drive expression of a cas cDNA containing the open reading frame.

(Kambadur et al., 1998)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

cas

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates brain cancer

modeled by pros^JF02308

(Gaultier et al., 2022)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | embryonic stage, with Scer\GAL4^en-e16E

(Grosskortenhaus et al., 2006)

abnormal neuroanatomy | embryonic stage, with Scer\GAL4^pros.PMG

(Grosskortenhaus et al., 2006)

decreased cell number | embryonic stage, with Scer\GAL4^en-e16E

(Fox et al., 2022)

Phenotype Manifest In

Detailed Description

Statement

Reference

Animals expressing cas^Scer\UAS.cKa under the control of Scer\GAL4^elav-C155 have an increase in the number of SELK neurons compared to control animals.

(Losada-Pérez et al., 2010)

Embryos expressing cas^Scer\UAS.cKa under the control of Scer\GAL4^en-e16E lack U4 and U5 neurons, although U1-U3 neuron fates are normal.

Embryos expressing cas^Scer\UAS.cKa under the control of Scer\GAL4^pros.PMG lack U4 and U5 neurons.

(Grosskortenhaus et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Antp^UAS.cMb, Scer\GAL4^GMR42G10, cas^UAS.cKa has abnormal neuroanatomy | embryonic stage phenotype, enhanceable by E(spl)m8-HLH^{CK2.UAS.Tag:FLAG}, Scer\GAL4^GMR42G10

(Bivik et al., 2016)

Enhancer of

Statement

Reference

cas^UAS.cKa/Antp^UAS.cMb, Scer\GAL4^GMR42G10 is an enhancer of abnormal neuroanatomy | embryonic stage phenotype of E(spl)m8-HLH^{CK2.UAS.Tag:FLAG}, Scer\GAL4^GMR42G10

(Bivik et al., 2016)

Suppressor of

Statement

Reference

Scer\GAL4^en-e16E/cas^UAS.cKa is a suppressor of increased cell number | embryonic stage phenotype of Cph¹

(Fox et al., 2022)

Scer\GAL4^en-e16E/cas^UAS.cKa is a suppressor of abnormal neuroanatomy phenotype of Scer\GAL4^en-e16E, hb^UAS.cWa

(Tran et al., 2010)

Scer\GAL4^elav-C155/cas^UAS.cKa is a suppressor of increased cell death phenotype of Scer\GAL4^elav-C155, abd-A^1dB5.UAS

(Maurange et al., 2008)

Other

Statement

Reference

Antp^UAS.cMb, Scer\GAL4^da.G32, cas^UAS.cKa, grh^UAS.cBa has decreased occurrence of cell division | embryonic stage 12 phenotype

(Bahrampour et al., 2017)

Antp^UAS.cMb, Scer\GAL4^GMR42G10, cas^UAS.cKa has abnormal neuroanatomy | embryonic stage phenotype

(Bivik et al., 2016)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Antp^UAS.cMb, Scer\GAL4^GMR42G10, cas^UAS.cKa has thoracic neuroblast | embryonic stage phenotype, non-enhanceable by E(spl)m8-HLH^{CK2.UAS.Tag:FLAG}, Scer\GAL4^GMR42G10

(Bivik et al., 2016)

Antp^UAS.cMb, Scer\GAL4^GMR42G10, cas^UAS.cKa has abdominal neuroblast | embryonic stage phenotype, non-enhanceable by E(spl)m8-HLH^{CK2.UAS.Tag:FLAG}, Scer\GAL4^GMR42G10

(Bivik et al., 2016)

NOT Enhancer of

Statement

Reference

cas^UAS.cKa/Antp^UAS.cMb, Scer\GAL4^GMR42G10 is a non-enhancer of thoracic neuroblast | embryonic stage phenotype of E(spl)m8-HLH^{CK2.UAS.Tag:FLAG}, Scer\GAL4^GMR42G10

(Bivik et al., 2016)

Suppressor of

Statement

Reference

Scer\GAL4^en-e16E/cas^UAS.cKa is a suppressor of U neuron | increased number | embryonic stage phenotype of Cph¹

(Fox et al., 2022)

Scer\GAL4^en-e16E/cas^UAS.cKa is a suppressor of U neuron | increased number phenotype of Scer\GAL4^en-e16E, hb^UAS.cWa

(Tran et al., 2010)

Scer\GAL4^elav-C155/cas^UAS.cKa is a suppressor of neuroblast phenotype of Scer\GAL4^elav-C155, abd-A^1dB5.UAS

(Maurange et al., 2008)

Other

Statement

Reference

Antp^UAS.cMb, Scer\GAL4^da.G32, cas^UAS.cKa, grh^UAS.cBa has embryonic neuroblast | embryonic stage 12 phenotype

(Bahrampour et al., 2017)

Antp^UAS.cMb, Scer\GAL4^da.G32, cas^UAS.cKa, grh^UAS.cBa has embryonic ganglion mother cell | embryonic stage 12 phenotype

(Bahrampour et al., 2017)

Antp^UAS.cMb, Scer\GAL4^GMR42G10, cas^UAS.cKa has thoracic neuroblast | embryonic stage phenotype

(Bivik et al., 2016)

Antp^UAS.cMb, Scer\GAL4^GMR42G10, cas^UAS.cKa has abdominal neuroblast | embryonic stage phenotype

(Bivik et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

The triple co-expression of cas^Scer\UAS.cKa, grh^Scer\UAS.cBa and Antp^Scer\UAS.cMb triggers an apparent reduction in the numbers of dividing neuroblasts and dividing neuroblast daughters in stage 12 embryos, as compared to controls.

(Bahrampour et al., 2017)

Co-expression of cas^Scer\UAS.cKa and Antp^Scer\UAS.cMb under the control of Scer\GAL4^GMR42G10 results in a significant reduction in thoracic neuroblast proliferation at stage 12 (but not 14), neuroblast daughter cell proliferation at stage 14 (but not stage 12), and abdominal neuroblast and neuroblast daughter cell proliferation at both stage 12 and 14, as compared with controls.

Co-expression of E(spl)m8-HLH^{CK2.Scer\UAS.T:Zzzz\FLAG}, cas^Scer\UAS.cKa and Antp^Scer\UAS.cMb under the control of Scer\GAL4^GMR42G10 enhances the reduced proliferation phenotype in neuroblast daughter cells, but does not enhance the reduced proliferation of neuroblasts, as compared with embryos expressing cas^Scer\UAS.cKa and Antp^Scer\UAS.cMb under the control of Scer\GAL4^GMR42G10.

(Bivik et al., 2016)

Co-expression of hb^Scer\UAS.cWa and cas^Scer\UAS.cKa in neuroblast NB7-1 under the control of Scer\GAL4^en-e16E produces an average of fifteen U neurons per hemisegment.

(Tran et al., 2010)

The neuroblast apoptosis seen in the thorax and abdomen of 96 hour larvae expressing abd-A^{1dB5.Scer\UAS} under the control of Scer\GAL4^elav-C155 is suppressed by co-expression of cas^Scer\UAS.cKa.

(Maurange et al., 2008)

Co-expression of cas^Scer\UAS.cKa and pdm2^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^en-e16E results in embryos which have normal U1/U2 neurons, but show a loss of the U3/U4 neurons and extra U5 neurons.

(Grosskortenhaus et al., 2006)

Xenogenetic Interactions

Statement

Reference

Co-expression of BacA\p35^Scer\UAS.cHa and cas^Scer\UAS.cKa under the control of Scer\GAL4^en-e16E results in loss of U4/U5 neurons (as is seen in embryos expressing cas^Scer\UAS.cKa alone under the control of Scer\GAL4^en-e16E).

(Grosskortenhaus et al., 2006)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

cas^Scer\UAS.cKa

cas^UAS.cKa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Kambadur

(Kambadur et al., 1998)

Secondary FlyBase IDs

References (18)

Report Sections

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