FlyBase Allele Report: Dmel\hb[P1only]

General Information

Symbol

Dmel\hb^P1only

Species

D. melanogaster

Name

FlyBase ID

FBal0117499

Feature type

allele

Associated gene

Dmel\hb

Associated Insertion(s)

Carried in Construct

P{hb.P1only}

Also Known As

hb^P1

Key Links

Transgenic product class

wild_type

(Wimmer et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Wimmer et al., 2000)

Mutagen

in vitro construct

(Wimmer et al., 2000)

Progenitor genotype

Carried in construct

P{hb.P1only}

Cytology

Description

Intron between P1 promoter and the hb coding region, which includes the P2 promoter, has been removed, leaving the hb coding region to be expressed from the P1 promoter.

(Wimmer et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy (with hb¹⁵), with Scer\GAL4^en-e16E, hb^UAS.cWa

(Tran et al., 2010)

abnormal neuroanatomy (with hb¹⁵), with Scer\GAL4^en-e16E, hb^UAS.VP16

(Tran et al., 2010)

abnormal neuroanatomy (with hb¹⁵), with Scer\GAL4^en-e16E, hb^{ΔAB.VP16.UAS}

(Tran et al., 2010)

abnormal neuroanatomy (with hb¹⁵), with Scer\GAL4^en-e16E, hb^ΔB.VP16.UAS

(Tran et al., 2010)

abnormal neuroanatomy (with hb¹⁵), with Scer\GAL4^en-e16E, hb^ΔD.VP16.UAS

(Tran et al., 2010)

abnormal neuroanatomy (with hb¹⁵), with Scer\GAL4^en-e16E, hb^{ΔDMZ.VP16.UAS}

(Tran et al., 2010)

abnormal neuroanatomy (with hb¹⁵), with Scer\GAL4^en-e16E, hb^ΔE.VP16.UAS

(Tran et al., 2010)

decreased occurrence of cell division | embryonic stage 13, with hb¹⁵

(Bahrampour et al., 2017)

decreased occurrence of cell division | embryonic stage 14, with hb¹⁵

(Bahrampour et al., 2017)

Phenotype Manifest In

embryonic ganglion mother cell | embryonic stage 13, with hb¹⁵

(Bahrampour et al., 2017)

embryonic neuroblast | embryonic stage 13, with hb¹⁵

(Bahrampour et al., 2017)

embryonic neuroblast | embryonic stage 14, with hb¹⁵

(Bahrampour et al., 2017)

larval DA2 motor neuron | increased number (with hb¹⁵), with Scer\GAL4^en-e16E, hb^ΔD.VP16.UAS

(Tran et al., 2010)

larval DA2 motor neuron | increased number (with hb¹⁵), with Scer\GAL4^en-e16E, hb^{ΔDMZ.VP16.UAS}

(Tran et al., 2010)

larval DO1 motor neuron (with hb¹⁵), with Scer\GAL4^en-e16E, hb^ΔD.VP16.UAS

(Tran et al., 2010)

larval DO1 motor neuron (with hb¹⁵), with Scer\GAL4^en-e16E, hb^{ΔDMZ.VP16.UAS}

(Tran et al., 2010)

larval DO2 motor neuron (with hb¹⁵), with Scer\GAL4^en-e16E, hb^ΔD.VP16.UAS

(Tran et al., 2010)

larval DO2 motor neuron (with hb¹⁵), with Scer\GAL4^en-e16E, hb^{ΔDMZ.VP16.UAS}

(Tran et al., 2010)

U neuron (with hb¹⁵), with Scer\GAL4^en-e16E, hb^UAS.VP16

(Tran et al., 2010)

U neuron | increased number (with hb¹⁵), with Scer\GAL4^en-e16E, hb^UAS.cWa

(Tran et al., 2010)

U neuron | increased number (with hb¹⁵), with Scer\GAL4^en-e16E, hb^{ΔAB.VP16.UAS}

(Tran et al., 2010)

U neuron | increased number (with hb¹⁵), with Scer\GAL4^en-e16E, hb^ΔB.VP16.UAS

(Tran et al., 2010)

U neuron | increased number (with hb¹⁵), with Scer\GAL4^en-e16E, hb^ΔE.VP16.UAS

(Tran et al., 2010)

Detailed Description

Statement

Reference

hb^P1only/hb¹⁵ transheterozygotes present a significant decrease in the number of dividing neuroblasts during stages 13 and 14 of embryogenesis and present a significant decrease in the number of dividing neuroblast daughters during stage 13, but not stage 14, of embryogenesis, as compared to controls; these embryos, however, show no significant differences in the number of neuroblasts, as compared to controls.

(Bahrampour et al., 2017)

In wild-type, neuroblast NB7-1 generates five U motoneurons. Overexpression of hb^{Scer\UAS.T:Hsim\VP16} in hb¹⁵/hb^P1only mutant NB7-1 under the control of Scer\GAL4^en-e16E produces an average of six U neurons per hemisegment.

In wild-type, neuroblast NB7-1 generates five U motoneurons. Overexpression of hb^Scer\UAS.cWa in hb¹⁵/hb^P1only mutant NB7-1 under the control of Scer\GAL4^en-e16E produces an average of 12 U neurons per hemisegment.

(Tran et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

hb^P1only partially rescues hb¹⁵/hb¹²

(Wimmer et al., 2000)

Comments

When one copy of hb^P1only is supplied zygotically (via the father) to hb¹²/hb¹⁵ embryos the A7/A8 fusion mutant phenotype is rescued. When one copy of hb^P1only is supplied maternally both the A7/A8 and anterior labial and T1 segments are rescued; only T2 and T3 remain unrescued. When the maternal contribution of hb^P1only is increased (three copies) 30% rescued embryos show only the maternal rescue of labial and T1 segments. Most show additional rescue of thoracic segments of mostly T3 character. 5-10% of embryos show rescue of all thoracic segments.

(Wimmer et al., 2000)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Removal of the bcd-dependent P2 promoter uncouples the direct link between the bcd and hb morphogen systems.

(Wimmer et al., 2000)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

hb^P1

(Bahrampour et al., 2019, Bahrampour et al., 2017, Gabilondo et al., 2016, Benito-Sipos et al., 2010, Losada-Pérez et al., 2010, Tran et al., 2010, Isshiki et al., 2001)

hb^P1only

Name Synonyms

Secondary FlyBase IDs

References (8)

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