FlyBase Allele Report: Hsap\APP[695ΔCT.UAS.Tag:MYC]

General Information

Symbol

Hsap\APP^{695ΔCT.UAS.Tag:MYC}

Species

H. sapiens

Name

FlyBase ID

FBal0095696

Feature type

allele

Associated gene

Hsap\APP

Associated Insertion(s)

Carried in Construct

P{UAS-APP.695ΔCT.T:Myc}

Also Known As

UAS-APPΔCT, UAS-APP dCT, APP dCT, UAS-APP695^ΔCT

Key Links

Transgenic product class

inframe_deletion

(Fossgreen et al., 1998)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Fossgreen et al., 1998)

Mutagen

in vitro construct

(Fossgreen et al., 1998)

Progenitor genotype

Carried in construct

P{UAS-APP.695ΔCT.T:Myc}

Cytology

Description

A mutant form of Hsap\APP which lacks the cytoplasmic tail (but contains the signal peptide, extracellular domain and βA4 region) tagged in the N-terminal region with Tag:MYC is expressed under the control of UASt regulatory sequences.

(Fossgreen et al., 1998)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Hsap\APP

Tagged with

Tag:MYC

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | larval stage, with Scer\GAL4^ppk.PU

(Pizzano et al., 2023)

viable, with Scer\GAL4^337Y

(Pirooznia et al., 2012)

Phenotype Manifest In

axon | larval stage, with Scer\GAL4^ppk.PU

(Pizzano et al., 2023)

larval multidendritic class IV neuron, with Scer\GAL4^ppk.PU

(Pizzano et al., 2023)

Detailed Description

Statement

Reference

Males expressing Hsap\APP^{695ΔCT.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Gr33a-GAL4 show no difference in courtship intensity or preference for younger females as compared with controls.

(Hu et al., 2015)

Flies with Hsap\APP^{695ΔCT.Scer\UAS.T:Hsap\MYC} overexpressed by Scer\GAL4^Appl.G1a do not have wing expansion defects.

(Peng et al., 2015)

Expression of Hsap\APP^{695ΔCT.Scer\UAS.T:Hsap\MYC} fails to induce cell death in the central nervous system (under the control of Scer\GAL4^elav-C155), the developing eye (Scer\GAL4^GMR.PF), thorax (Scer\GAL4^pnr-MD237), and wing (Scer\GAL4^ptc-559.1).

Expression of Hsap\APP^{695ΔCT.Scer\UAS.T:Hsap\MYC} in the central nervous system, under the control of Scer\GAL4^Appl.G1a, does not alter levels of cell death nor locomotory ability.

(Wang et al., 2014)

Scer\GAL4^ey-OK107>Hsap\APP^{695ΔCT.Scer\UAS.T:Hsap\MYC} male flies show normal learning in a conditioned courtship suppression assay (placed with a mated female for 60 minutes), with a significant drop (similar to wild type) in courtship index when comparing the first 10 minutes to the final 10 minutes. Two minutes after training, flies are placed with a virgin female and courtship is assessed for 10 minutes: trained controls show significantly reduced courtship index when compared to sham (untrained males), just like Scer\GAL4^ey-OK107>Hsap\APP^{695ΔCT.Scer\UAS.T:Hsap\MYC} males (immediate-recall memory intact).

(Xu et al., 2014)

Expression of Hsap\APP^{695ΔCT.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^P2.4.Pdf has no significant effect on the axonal pattern of the s-LNv neurons either in third instar larval or in adult brains or on sleep pattern in adult flies.

(Pirooznia et al., 2012)

Expression of Hsap\APP^{695ΔCT.Scer\UAS.T:Hsap\MYC}, under the control of Scer\GAL4^P2.4.Pdf, does not induce a significant increase in axonal arborization of the sLNv.

(Leyssen et al., 2005)

When Hsap\APP^{695-Swedish.Scer\UAS} is driven by Scer\GAL4^Appl.G1a or Scer\GAL4^179Y no accumulation of organelles is seen in larval axons.

(Gunawardena and Goldstein, 2001)

Flies expressing Hsap\APP^{695ΔCT.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Mz1087.hx do not have a wing blister phenotype.

(Fossgreen et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

Hsap\APP^{695ΔCT.UAS.Tag:MYC}, Scer\GAL4^P2.4.Pdf is a non-enhancer of abnormal neuroanatomy | adult stage phenotype of Scer\GAL4^P2.4.Pdf, Tip60^E431Q.UAS

(Pirooznia et al., 2012)

NOT Suppressor of

Statement

Reference

Hsap\APP^{695ΔCT.UAS.Tag:MYC}, Scer\GAL4^P2.4.Pdf is a non-suppressor of abnormal sleep | adult stage phenotype of Scer\GAL4^P2.4.Pdf, Tip60^UAS.cLa

(Pirooznia et al., 2012)

Hsap\APP^{695ΔCT.UAS.Tag:MYC}, Scer\GAL4^337Y is a non-suppressor of lethal phenotype of Scer\GAL4^337Y, Tip60^E431Q.UAS

(Pirooznia et al., 2012)

Hsap\APP^{695ΔCT.UAS.Tag:MYC}, Scer\GAL4^337Y is a non-suppressor of majority die during third instar larval stage phenotype of Scer\GAL4^337Y, Tip60^E431Q.UAS

(Pirooznia et al., 2012)

Hsap\APP^{695ΔCT.UAS.Tag:MYC}/Scer\GAL4^179Y is a non-suppressor of lethal phenotype of Scer\GAL4^337Y, Tip60^E431Q.UAS

(Pirooznia et al., 2012)

Hsap\APP^{695ΔCT.UAS.Tag:MYC}/Scer\GAL4^179Y is a non-suppressor of majority die during third instar larval stage phenotype of Scer\GAL4^337Y, Tip60^E431Q.UAS

(Pirooznia et al., 2012)

Hsap\APP^{695ΔCT.UAS.Tag:MYC}, Scer\GAL4^337Y is a non-suppressor of partially lethal - majority die phenotype of Scer\GAL4^337Y, Tip60^UAS.cPa

(Pirooznia et al., 2012)

Hsap\APP^{695ΔCT.UAS.Tag:MYC}, Scer\GAL4^179Y is a non-suppressor of partially lethal - majority die phenotype of Scer\GAL4^179Y, Tip60^UAS.cPa

(Pirooznia et al., 2012)

Other

Statement

Reference

Hsap\APP^{695ΔCT.UAS.Tag:MYC}, Scer\GAL4^ey-OK107, Tip60^UAS.cLa has abnormal memory | adult stage phenotype

(Xu et al., 2014)

Hsap\APP^{695ΔCT.UAS.Tag:MYC}, Scer\GAL4^ey-OK107, Tip60^E431Q.UAS has abnormal memory | adult stage phenotype

(Xu et al., 2014)

Hsap\APP^{695ΔCT.UAS.Tag:MYC}, Scer\GAL4^P2.4.Pdf, Tip60^E431Q.UAS has abnormal sleep | adult stage phenotype

(Pirooznia et al., 2012)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Hsap\APP^{695ΔCT.UAS.Tag:MYC}, Scer\GAL4^Appl.G1a has embryonic/larval nervous system phenotype, non-enhanceable by Df(3L)GN24

(Gunawardena and Goldstein, 2001)

Hsap\APP^{695ΔCT.UAS.Tag:MYC}, Scer\GAL4^Appl.G1a has embryonic/larval nervous system phenotype, non-enhanceable by robl^K

(Gunawardena and Goldstein, 2001)

NOT suppressed by

Statement

Reference

Hsap\APP^{695ΔCT.UAS.Tag:MYC}, Scer\GAL4^Appl.G1a has embryonic/larval nervous system phenotype, non-suppressible by Df(3L)GN24

(Gunawardena and Goldstein, 2001)

Hsap\APP^{695ΔCT.UAS.Tag:MYC}, Scer\GAL4^Appl.G1a has embryonic/larval nervous system phenotype, non-suppressible by robl^K

(Gunawardena and Goldstein, 2001)

NOT Enhancer of

Statement

Reference

Hsap\APP^{695ΔCT.UAS.Tag:MYC}, Scer\GAL4^P2.4.Pdf is a non-enhancer of s-LNv Pdf neuron | adult stage phenotype of Scer\GAL4^P2.4.Pdf, Tip60^E431Q.UAS

(Pirooznia et al., 2012)

Hsap\APP^{695ΔCT.UAS.Tag:MYC}, Scer\GAL4^P2.4.Pdf is a non-enhancer of axon | adult stage phenotype of Scer\GAL4^P2.4.Pdf, Tip60^E431Q.UAS

(Pirooznia et al., 2012)

Hsap\APP^{695ΔCT.UAS.Tag:MYC}/Scer\GAL4^Appl.G1a is a non-enhancer of embryonic/larval nervous system phenotype of Klc^8ex94

(Gunawardena and Goldstein, 2001)

Hsap\APP^{695ΔCT.UAS.Tag:MYC}/Scer\GAL4^Appl.G1a is a non-enhancer of embryonic/larval nervous system phenotype of Khc⁸

(Gunawardena and Goldstein, 2001)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Co-expression of Tip60^Scer\UAS.cLa or Tip60^{E431Q.Scer\UAS} does not affect learning but abolishes short-term memory of courtship suppression in Scer\GAL4^ey-OK107>Hsap\APP^{695ΔCT.Scer\UAS.T:Hsap\MYC} males.

(Xu et al., 2014)

The defects in the axonal pattern of s-LNv neurons in brains characteristic for adult flies expressing Tip60^{E431Q.Scer\UAS} under the control of Scer\GAL4^P2.4.Pdf are not exacerbated by co-expression of Hsap\APP^{695ΔCT.Scer\UAS.T:Hsap\MYC}; flies co-expressing Tip60^{E431Q.Scer\UAS} with Hsap\APP^{695ΔCT.Scer\UAS.T:Hsap\MYC} show reduction in nighttime sleep with concomitant increase in daytime sleep (also seen in flies expressing Tip60^{E431Q.Scer\UAS} alone).

Simultaneous expression of Tip60^Scer\UAS.cLa with Hsap\APP^{695ΔCT.Scer\UAS.T:Hsap\MYC} has no significant effect on the axonal pattern of the s-LNv neurons either in larval or in adult brains and it does not rescue but rather enhances the nighttime sleep deficit observed in flies expressing Tip60^Scer\UAS.cLa alone.

(Pirooznia et al., 2012)

The addition of Df(3L)GN24 to Hsap\APP^{695ΔCT.Scer\UAS.T:Hsap\MYC}, Scer\GAL4^Appl.G1a larvae has no effect on the larval axons. The addition of robl^K to Hsap\APP^{695ΔCT.Scer\UAS.T:Hsap\MYC}, Scer\GAL4^Appl.G1a larvae has no effect on the larval axons.

(Gunawardena and Goldstein, 2001)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Carried in a plasmid and transfected into SL-2 cells with a Scer\GAL4 expression plasmid. The expression and metabolism of Hsap\APP^{695ΔCT.Scer\UAS.T:Hsap\MYC} protein in these cells has been studied.

(Fossgreen et al., 1998)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Hsap\APP^{695ΔCT.Scer\UAS.T:Hsap\MYC}

Hsap\APP^{695ΔCT.UAS.Tag:MYC}

Name Synonyms

Secondary FlyBase IDs

References (12)

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