FlyBase Allele Report: Dmel\Klc[8ex94]

General Information

Symbol

Dmel\Klc^8ex94

Species

D. melanogaster

Name

FlyBase ID

FBal0089617

Feature type

allele

Associated gene

Dmel\Klc

Associated Insertion(s)

Carried in Construct

Also Known As

Df(3L)8ex94

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

Imprecise excision of a P-element, deleting most of the Klc transcription unit.

(Gindhart et al., 1998)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates tauopathy

modeled by Hsap\MAPT^R406W.UAS

(Falzone et al., 2010)

modeled by Hsap\MAPT^UAS.cWa

(Falzone et al., 2010)

exacerbates neurodegenerative disease

modeled by htt^RNAi.UAS

(Gunawardena et al., 2003)

exacerbates Alzheimer's disease

modeled by Hsap\APP^695.T.UAS

(Gunawardena and Goldstein, 2001)

modeled by Hsap\APP^{695-Swedish.UAS}

(Gunawardena and Goldstein, 2001)

modeled by Hsap\APP^{695SPA4CT.UAS.Tag:MYC}

(Gunawardena and Goldstein, 2001)

modeled by Hsap\APP^{V717F.LOND.UAS}

(Gunawardena and Goldstein, 2001)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of larval stage | recessive

(Gindhart et al., 1998)

some die during larval stage | recessive

(Gindhart et al., 1998)

Phenotype Manifest In

Detailed Description

Statement

Reference

Klc^8ex94/+ flies do not exhibit significantly increased bang sensitivity, nor any significant difference in seizure threshold or spikes per discharge in an electroconvulsive stimulation paradigm, as compared to wild type.

(Ehaideb et al., 2014)

Klc^8ex94/Klc^c02312 transheterozygotes show a partial disruption of the internal structure of the third instar larval mushroom body, as compared to controls.

Klc^8ex94 homozygous clones within mosaic third instar larval mushroom bodies show prevalent peduncle defects (split fascicles are observed in the majority of cases) and fully prevalent defects in the extension of calix dendrites, as compared to control clones.

(Mochizuki et al., 2011)

Homozygous null Klc^8ex94 eye clones in third instar larvae and adults are somewhat small and roughened, with axons that are frequently disordered and sometimes short and defasciculated, despite photoreceptors maintaining viability and undergoing normal differentiation. Axonal mitochondria are numerous, as in controls.

(Glater et al., 2006)

Ooplasmic streaming still occurs in mutant stage 9 and stage 10b oocytes, although the movements are often less vigorous than in wild type.

(Palacios and St. Johnston, 2002)

Homozygotes exhibit organelle accumulation in larval neurons. Heterozygotes do not show an organelle jamming phenotype.

(Gunawardena and Goldstein, 2001)

Homozygotes die at the boundary of the second and third larval instar stages. Klc^8ex94/Klc^59A animals survive until the late third larval instar stage. Klc^8ex94/Klc^59A larvae flip their posterior end off the surface of the food during crawling.

(Gindhart et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Klc[+]/Klc^8ex94 is an enhancer of partially lethal phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^Appl.G1a

(Falzone et al., 2010)

Klc[+]/Klc^8ex94 is an enhancer of partially lethal phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^Appl.G1a

(Falzone et al., 2010)

Klc^8ex94 is an enhancer of increased cell death phenotype of Hsap\APP^{695-Swedish.UAS}, Scer\GAL4^Appl.G1a

(Gunawardena and Goldstein, 2001)

Klc^8ex94 is an enhancer of increased cell death phenotype of Hsap\APP^{V717F.LOND.UAS}, Scer\GAL4^Appl.G1a

(Gunawardena and Goldstein, 2001)

Klc^8ex94 is an enhancer of increased cell death phenotype of Hsap\APP^{695SPA4CT.UAS.Tag:MYC}, Scer\GAL4^Appl.G1a

(Gunawardena and Goldstein, 2001)

Klc^8ex94 is an enhancer of increased cell death phenotype of Hsap\APP^695.T.UAS, Scer\GAL4^Appl.G1a

(Gunawardena and Goldstein, 2001)

Suppressor of

Statement

Reference

Klc[+]/Klc^8ex94 is a suppressor of bang sensitive phenotype of pk^sple-1

(Ehaideb et al., 2014)

Klc[+]/Klc^8ex94 is a suppressor of abnormal neurophysiology | adult stage phenotype of pk^sple-1

(Ehaideb et al., 2014)

Phenotype Manifest In

Enhanced by

Statement

Reference

Klc^8ex94 has embryonic/larval nervous system phenotype, enhanceable by Hsap\APP^{695-Swedish.UAS}/Scer\GAL4^Appl.G1a

(Gunawardena and Goldstein, 2001)

Klc^8ex94 has embryonic/larval nervous system phenotype, enhanceable by Hsap\APP^{695SPA4CT.UAS.Tag:MYC}/Scer\GAL4^Appl.G1a

(Gunawardena and Goldstein, 2001)

Klc^8ex94 has embryonic/larval nervous system phenotype, enhanceable by Scer\GAL4^Appl.G1a/Hsap\APLP2::Hsap\APP^UAS.Tag:MYC

(Gunawardena and Goldstein, 2001)

Klc^8ex94 has embryonic/larval nervous system phenotype, enhanceable by Scer\GAL4^Appl.G1a/Hsap\APP^695.T.UAS

(Gunawardena and Goldstein, 2001)

Klc^8ex94 has embryonic/larval nervous system phenotype, enhanceable by Scer\GAL4^Appl.G1a/Appl^UAS.cTa

(Gunawardena and Goldstein, 2001)

NOT Enhanced by

Statement

Reference

Klc^8ex94 has embryonic/larval nervous system phenotype, non-enhanceable by Appl^s.UAS/Scer\GAL4^Appl.G1a

(Gunawardena and Goldstein, 2001)

Klc^8ex94 has embryonic/larval nervous system phenotype, non-enhanceable by Scer\GAL4^Appl.G1a/Appl^sdΔIC.UAS

(Gunawardena and Goldstein, 2001)

Klc^8ex94 has embryonic/larval nervous system phenotype, non-enhanceable by Hsap\APP^{695ΔCT.UAS.Tag:MYC}/Scer\GAL4^Appl.G1a

(Gunawardena and Goldstein, 2001)

Enhancer of

Statement

Reference

Klc[+]/Klc^8ex94 is an enhancer of axon | larval stage phenotype of Scer\GAL4^Appl.G1a, htt^RNAi.UAS

(Gunawardena et al., 2003)

Klc[+]/Klc^8ex94 is an enhancer of dorsal appendage phenotype of DCTN2-p50^hs.PD

(Duncan and Warrior, 2002)

Other

Statement

Reference

Atg1²⁵, Klc[+]/Klc^8ex94 has dendrite | third instar larval stage phenotype

(Mochizuki et al., 2011)

Atg1²⁵, Klc[+]/Klc^8ex94 has embryonic/larval mushroom body | third instar larval stage phenotype

(Mochizuki et al., 2011)

Atg1²⁵, Klc[+]/Klc^8ex94 has calyx of larval mushroom body | third instar larval stage phenotype

(Mochizuki et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Klc^8ex94/+ fully suppresses the bang sensitivity and lowered seizure threshold phenotypes of pk^sple-1/+ mutants.

(Ehaideb et al., 2014)

Atg1²⁵, Klc^8ex94 heterozygous third instar larval mushroom bodies show defects in the extension of calix dendrites, but no obvious core integrity, as compared to either heterozygous controls.

(Mochizuki et al., 2011)

Heterozygosity for Klc^8ex94 dramatically increases the percentage of eggs laid by heat shocked Dmn^hs.PD flies with displaces and/or highly reduced dorsal appendages.

(Duncan and Warrior, 2002)

The addition of Appl^Scer\UAS.cTa to Klc^8ex94/+ animals causes the organelle jamming phenotype to be seen in this animals, thus enhancing the phenotype. The addition of Appl^{sdΔC.Scer\UAS} or Appl^s.Scer\UAS has no effect on this phenotype.

(Gunawardena and Goldstein, 2001)

Xenogenetic Interactions

Statement

Reference

Klc^8ex94/+ enhances the partialy lethality of individuals expressing either Hsap\MAPT^Scer\UAS.cWa or Hsap\MAPT^{R406W.Scer\UAS} under the control of Scer\GAL4^Appl.G1a.

(Falzone et al., 2010)

The addition of Hsap\APP^{695-Swedish.Scer\UAS}, Hsap\APP^{695SPA4CT.Scer\UAS.T:Hsap\MYC}, Hsap\APLP2::Hsap\APP^{Scer\UAS.T:Hsap\MYC}, or Hsap\APP^{695.T.Scer\UAS} (when driven by Scer\GAL4^Appl.G1a) to Klc^8ex94/+ animals causes the organelle jamming phenotype to be seen in this animals, thus enhancing the phenotype. The addition of Hsap\APP^{695ΔCT.Scer\UAS.T:Hsap\MYC} has no effect on this phenotype.

(Gunawardena and Goldstein, 2001)

Complementation and Rescue Data

Partially rescued by

Klc^59A/Klc^8ex94 is partially rescued by Klc^{Ubi-p63E.Tag:MYC}

(Gindhart et al., 1998)

Klc^59A/Klc^8ex94 is partially rescued by Klc^GEN

(Gindhart et al., 1998)

Klc^8ex94 is partially rescued by Klc^{Ubi-p63E.Tag:MYC}

(Gindhart et al., 1998)

Klc^8ex94 is partially rescued by Klc^GEN

(Gindhart et al., 1998)

Comments

Klc^GEN rescues homozygous and Klc^8ex94/Klc^59A animals to adulthood. Klc^{Ubi-p63E.T:Hsap\MYC} rescues Klc^8ex94/Klc^59A animals to adulthood. Klc^{Ubi-p63E.T:Hsap\MYC} allows homozygotes to survive to the late third larval instar stage, although these animals never enter the wandering stage that occurs before pupariation. Klc^8ex94/Klc^59A larvae flip their posterior end off the surface of the food during crawling. This phenotype is rescued by Klc^GEN and Klc^{Ubi-p63E.T:Hsap\MYC}.

(Gindhart et al., 1998)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

31997

w^*; Klc^8ex94/TM3, Sb¹

Notes on Origin

Discoverer

Comments

Complements lethal mutations in Ptp69D.

(Gindhart et al., 1998)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Df(3L)8ex94

(Duncan and Warrior, 2002, Gunawardena and Goldstein, 2001, Martin et al., 1999, Ray et al., 1999, Gindhart et al., 1998)

Df(3L)8ex⁹⁴

(Gunawardena et al., 2003)

Klc^8ex94

(Ehaideb et al., 2014, Schulman et al., 2014, Loiseau et al., 2010, Toda et al., 2008)

Klc^8x94

(Mochizuki et al., 2011)

klc^8ex94

(Li et al., 2023, White et al., 2020, Hansen et al., 2019, Hayashi et al., 2014, Kang et al., 2014, Gunawardena et al., 2013, Metzger et al., 2012, Falzone et al., 2010, Mische et al., 2007, Glater et al., 2006, Palacios and St. Johnston, 2002)

Name Synonyms

Secondary FlyBase IDs

References (22)

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