FlyBase Allele Report: Dmel\htt[RNAi.UAS]

General Information

Symbol

Dmel\htt^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0155191

Feature type

allele

Associated gene

Dmel\htt

Associated Insertion(s)

Carried in Construct

P{UAS-htt.RNAi}

Also Known As

UAS-htt-RNAi

Key Links

Transgenic product class

RNAi_reagent

(Gunawardena et al., 2003)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Gunawardena et al., 2003)

Mutagen

in vitro construct

(Gunawardena et al., 2003)

Progenitor genotype

Carried in construct

P{UAS-htt.RNAi}

Cytology

Description

UASt sequences drive expression of a 1 kb inverted repeat fragment corresponding to the 5' end of the UAS gene with a 300bp linker separating them.

(Gunawardena et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

htt

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of neurodegenerative disease

CEA

(Mugat et al., 2008, Gunawardena et al., 2003)

model of Huntington's disease

CEA

(White et al., 2020)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Huntington's disease

modeled by Hsap\HTT^{171aa.138Q.UAS.Tag:HA}

(Mugat et al., 2008)

model of neurodegenerative disease

is ameliorated by Hsap\HTT^Q0.UAS

(Mugat et al., 2008)

is exacerbated by Klc^8ex94

(Gunawardena et al., 2003)

is exacerbated by Khc⁹

(Gunawardena et al., 2003)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | progressive, with Scer\GAL4^GMR.PF

(Mugat et al., 2008)

visible | progressive, with Scer\GAL4^GMR.PF

(Mugat et al., 2008)

Phenotype Manifest In

axon | larval stage, with Scer\GAL4^Appl.G1a

(Gunawardena et al., 2003)

embryonic/larval brain, with Scer\GAL4^Appl.G1a

(Gunawardena et al., 2003)

eye, with Scer\GAL4^GMR.PS

(Gunawardena et al., 2003)

eye | progressive, with Scer\GAL4^GMR.PF

(Mugat et al., 2008)

mitotic spindle | third instar larval stage, with Scer\GAL4^insc.PU

(Godin et al., 2010)

neuroblast | third instar larval stage, with Scer\GAL4^insc.PU

(Godin et al., 2010)

ommatidium, with Scer\GAL4^GMR.PS

(Gunawardena et al., 2003)

ommatidium | progressive, with Scer\GAL4^GMR.PF

(Mugat et al., 2008)

Detailed Description

Statement

Reference

Expression of htt^{dsRNA.Scer\UAS} under the control of Scer\GAL4^insc.PU results in mitotic spindle positioning defects in third instar larval neuroblasts: in contrast to wild-type controls, a significant proportion of cells show spindles with the angle between centrosomal pole and center of the mira protein crescent higher than the 15[o].

(Godin et al., 2010)

Newly hatched flies expressing Scer\GAL4^GMR.PF>htt^{dsRNA.Scer\UAS} have normal eyes. These flies show a progressive loss of pigmentation associated with a rough eye phenotype. The rough eye phenotype is characterised by abnormal ommatidial arrays.

(Mugat et al., 2008)

When htt^{dsRNA.Scer\UAS} is driven by Scer\GAL4^Appl.G1a larvae exhibit defects characteristic of axonal transport problems (organelles accumulate within larval nerves) as well as a small amount of cell death in the larval brain. When htt^{dsRNA.Scer\UAS} is driven by Scer\GAL4^GMR.PS, a rough eye phenotype is seen. This phenotype progresses over time, causing severe problems of morphology and pigmentation, leading to dark areas indicative of death. Large holes are visible in the most lateral part of the lamina.

(Gunawardena et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, htt^RNAi.UAS has visible | progressive phenotype, suppressible by Hsap\HTT^Q0.UAS, Scer\GAL4^GMR.PF

(Mugat et al., 2008)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^Appl.G1a, htt^RNAi.UAS has axon | larval stage phenotype, enhanceable by Klc[+]/Klc^8ex94

(Gunawardena et al., 2003)

Scer\GAL4^Appl.G1a, htt^RNAi.UAS has axon | larval stage phenotype, enhanceable by Khc[+]/Khc⁹

(Gunawardena et al., 2003)

NOT Enhanced by

Statement

Reference

Scer\GAL4^Appl.G1a, htt^RNAi.UAS has axon | larval stage phenotype, non-enhanceable by Df(2R)k10408/+

(Gunawardena et al., 2003)

Scer\GAL4^Appl.G1a, htt^RNAi.UAS has axon | larval stage phenotype, non-enhanceable by Df(3L)GN24/+

(Gunawardena et al., 2003)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, htt^RNAi.UAS has eye | progressive phenotype, suppressible by Hsap\HTT^Q0.UAS, Scer\GAL4^GMR.PF

(Mugat et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

htt^RNAi.UAS

htt^{dsRNA.Scer\UAS}

htt^dsRNA.UAS

huntingtin^{dsRNA.Scer\UAS}

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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