FlyBase Allele Report: Dmel\Atg1[25]

General Information

Symbol

Dmel\Atg1²⁵

Species

D. melanogaster

Name

FlyBase ID

FBal0220538

Feature type

allele

Associated gene

Dmel\Atg1

Associated Insertion(s)

Carried in Construct

Also Known As

unc-51²⁵

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

Nature of the Allele

Allele class

loss of function allele

(Toda et al., 2008)

Mutagen

Delta2-3 transposase

(Wairkar et al., 2009)

P-element activity

(Toda et al., 2008)

Progenitor genotype

Atg1^EP3348

(Wairkar et al., 2009, Toda et al., 2008)

Cytology

Description

Imprecise excision of P{EP}Atg1^EP3348has deleted 1787bp downstream from the insertion point.

(Wairkar et al., 2009)

Imprecise excision of the insertion in Atg1^EP3348, resulting in a deletion of 1787bp downstream from the insertion point.

(Toda et al., 2008)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3L:12,803,196..12,804,983 [-]

Comment:

Imprecise excision of the P{EP}Atg1^EP3348 insertion resulting in a deletion that extends 1787 bp downstream of the insertion site.

(Wairkar et al., 2009)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of pupal stage

(Wairkar et al., 2009)

lethal - all die before end of pupal stage (with Atg1³)

(Wairkar et al., 2009, Toda et al., 2008)

lethal - all die before end of pupal stage (with Df(3L)ED4486)

(Toda et al., 2008)

some die during pupal stage

(Wairkar et al., 2009)

some die during pupal stage (with Atg1³)

(Wairkar et al., 2009, Toda et al., 2008)

some die during pupal stage (with Df(3L)ED4486)

(Toda et al., 2008)

Phenotype Manifest In

Detailed Description

Statement

Reference

Atg1²⁵ mutants display basal autophagy defects in larval fat body cells.

(Pircs et al., 2012)

Atg1²⁵ homozygous third instar larval mushroom bodies show prevalent disintegration of the core into multiple bundles and defects in the extension of calix dendrites, as compared to controls. The mutants mushroom bodies also show defective axonal transport of the synaptic vesicle (monitored by the localization of nSyb::GFP and Syt::eGFP ) despite of no obvious defects in microtubule integrity (assessed by the pattern of Tau::GFP and Futsch), and show aberrant accumulation of early endosomes, late endosomes and Golgi apparatus in the cell body, calyx and lobes, but no defects in the localization of autophagosomes or of mitochondria, as compared to controls.

(Mochizuki et al., 2011)

Marker analysis indicates that pre-and post synaptic component of the neuromuscular junction fail to align properly in Atg1²⁵ homozygous or Atg1²⁵/Atg1³ heterozygous larvae.

(Wairkar et al., 2009)

Atg1³/Atg1²⁵ and Atg1²⁵/Df(3L)ED4486 animals show axonal transport defects at the larval stage.

(Toda et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Atg1²⁵, mask^UAS.cZa, Atg1[+], Scer\GAL4^how-24B is a suppressor | partially of lethal - all die before end of P-stage | heat sensitive phenotype of Prosβ6^1.B.UAS, Scer\GAL4^how-24B

(Zhu et al., 2017)

NOT Suppressor of

Statement

Reference

Atg1²⁵/Atg1³ is a non-suppressor of abnormal neuroanatomy | larval stage phenotype of fzy¹/fzy⁵⁰³²

(Kuang et al., 2014)

Atg1²⁵/Atg1³ is a non-suppressor of decreased cell number | larval stage phenotype of fzy¹/fzy⁵⁰³²

(Kuang et al., 2014)

Phenotype Manifest In

Suppressor of

Statement

Reference

Atg1²⁵, mask^UAS.cZa, Atg1[+], Scer\GAL4^how-24B is a suppressor | partially of pupal thorax | heat sensitive phenotype of Prosβ6^1.B.UAS, Scer\GAL4^how-24B

(Zhu et al., 2017)

NOT Suppressor of

Statement

Reference

Atg1²⁵/Atg1³ is a non-suppressor of neuroblast | larval stage phenotype of fzy¹/fzy⁵⁰³²

(Kuang et al., 2014)

Other

Statement

Reference

Atg1²⁵, Klc[+]/Klc^8ex94 has dendrite | third instar larval stage phenotype

(Mochizuki et al., 2011)

Atg1²⁵, Klc[+]/Klc^8ex94 has embryonic/larval mushroom body | third instar larval stage phenotype

(Mochizuki et al., 2011)

Atg1²⁵, Klc[+]/Klc^8ex94 has calyx of larval mushroom body | third instar larval stage phenotype

(Mochizuki et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Atg1²⁵/Atg1³ does not suppress the neuroblast loss seen in fzy⁵⁰³²/fzy¹ mutant larval brains.

(Kuang et al., 2014)

Atg1²⁵, Klc^8ex94 heterozygous third instar larval mushroom bodies show defects in the extension of calix dendrites, but no obvious core integrity, as compared to either heterozygous controls.

(Mochizuki et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Atg1³

(Toda et al., 2008)

Rescued by

Atg1²⁵/Atg1³ is rescued by Scer\GAL4^elav-C155/Atg1^UAS.cTa

(Toda et al., 2008)

Partially rescued by

Atg1²⁵ is partially rescued by Atg1^UAS.cTa

(Mochizuki et al., 2011)

Not rescued by

Atg1²⁵ is not rescued by Atg1^K38A.UAS

(Mochizuki et al., 2011)

Atg1²⁵/Atg1³ is not rescued by Scer\GAL4^elav-C155/Atg1^K38A.UAS

(Toda et al., 2008)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Atg1 ²⁵

(Pircs et al., 2012)

Atg1²⁵

(Billes et al., 2018, Pircs et al., 2012)

Atg1[25]

(Nagy et al., 2015)

unc-51²⁵

(Zhu et al., 2017, Kuang et al., 2014, Mochizuki et al., 2011, Wairkar et al., 2009, Toda et al., 2008)

Name Synonyms

Secondary FlyBase IDs

References (8)

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