FlyBase Allele Report: Hsap\APP[695SPA4CT.UAS.Tag:MYC]

General Information

Symbol

Hsap\APP^{695SPA4CT.UAS.Tag:MYC}

Species

H. sapiens

Name

FlyBase ID

FBal0095693

Feature type

allele

Associated gene

Hsap\APP

Associated Insertion(s)

Carried in Construct

P{UAS-APP.695SPA4CT.T:Myc}

Key Links

Transgenic product class

inframe_deletion

(Fossgreen et al., 1998)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Fossgreen et al., 1998)

Mutagen

in vitro construct

(Fossgreen et al., 1998)

Progenitor genotype

Carried in construct

P{UAS-APP.695SPA4CT.T:Myc}

Cytology

Description

A mutant form of Hsap\APP which lacks the extracellular domain (but contains the signal peptide, βA4 region and cytoplasmic tail) tagged at the C-terminus with Tag:MYC is expressed under the control of UASt regulatory sequences.

(Fossgreen et al., 1998)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Hsap\APP

Tagged with

Tag:MYC

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Alzheimer's disease

CEA

(Gunawardena and Goldstein, 2001)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of Alzheimer's disease

is ameliorated by robl^K

(Gunawardena and Goldstein, 2001)

is exacerbated by Khc⁸

(Gunawardena and Goldstein, 2001)

is exacerbated by Klc^8ex94

(Gunawardena and Goldstein, 2001)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal memory, with Scer\GAL4^{Mef2.247.Switch}

(Goguel et al., 2011)

abnormal neuroanatomy, with Scer\GAL4^P2.4.Pdf

(Leyssen et al., 2005)

increased cell death, with Scer\GAL4^Appl.G1a

(Gunawardena and Goldstein, 2001)

Phenotype Manifest In

embryonic/larval nervous system, with Scer\GAL4^179Y

(Gunawardena and Goldstein, 2001)

embryonic/larval nervous system, with Scer\GAL4^Appl.G1a

(Gunawardena and Goldstein, 2001)

ventral adult lateral neuron & axon, with Scer\GAL4^P2.4.Pdf

(Leyssen et al., 2005)

Detailed Description

Statement

Reference

Flies expressing Hsap\APP^{695SPA4CT.Scer\UAS.T:Hsap\MYC} in the adult mushroom bodies under the control of Scer\GAL4^{Mef2.247.Switch} exhibit a long-term memory defect.

(Goguel et al., 2011)

Expression of Hsap\APP^{695SPA4CT.Scer\UAS.T:Hsap\MYC}, under the control of Scer\GAL4^P2.4.Pdf, results in increased axonal arborization of the sLNv.

(Leyssen et al., 2005)

When Hsap\APP^{695-Swedish.Scer\UAS} is driven by Scer\GAL4^Appl.G1a or Scer\GAL4^179Y an occasional accumulation of organelles is seen in larval axons (about 10 clogs per 50μm). When Hsap\APP^{695SPA4CT.Scer\UAS.T:Hsap\MYC} is driven by Scer\GAL4^Appl.G1a, apoptosis is seen in the larval brain.

(Gunawardena and Goldstein, 2001)

Flies expressing Hsap\APP^{695SPA4CT.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Mz1087.hx do not have a wing blister phenotype.

(Fossgreen et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Hsap\APP^{695SPA4CT.UAS.Tag:MYC}, Scer\GAL4^Appl.G1a has increased cell death phenotype, enhanceable by Khc⁸

(Gunawardena and Goldstein, 2001)

Hsap\APP^{695SPA4CT.UAS.Tag:MYC}, Scer\GAL4^Appl.G1a has increased cell death phenotype, enhanceable by Klc^8ex94

(Gunawardena and Goldstein, 2001)

Suppressed by

Statement

Reference

Hsap\APP^{695SPA4CT.UAS.Tag:MYC}, Scer\GAL4^Appl.G1a has increased cell death phenotype, suppressible by Df(3L)GN24

(Gunawardena and Goldstein, 2001)

Hsap\APP^{695SPA4CT.UAS.Tag:MYC}, Scer\GAL4^Appl.G1a has increased cell death phenotype, suppressible by robl^K

(Gunawardena and Goldstein, 2001)

Phenotype Manifest In

Suppressed by

Statement

Reference

Hsap\APP^{695SPA4CT.UAS.Tag:MYC}, Scer\GAL4^Appl.G1a has embryonic/larval nervous system phenotype, suppressible by Df(3L)GN24

(Gunawardena and Goldstein, 2001)

Hsap\APP^{695SPA4CT.UAS.Tag:MYC}, Scer\GAL4^Appl.G1a has embryonic/larval nervous system phenotype, suppressible by robl^K

(Gunawardena and Goldstein, 2001)

Enhancer of

Statement

Reference

Hsap\APP^{695SPA4CT.UAS.Tag:MYC}/Scer\GAL4^Appl.G1a is an enhancer of embryonic/larval nervous system phenotype of Khc⁸

(Gunawardena and Goldstein, 2001)

Hsap\APP^{695SPA4CT.UAS.Tag:MYC}/Scer\GAL4^Appl.G1a is an enhancer of embryonic/larval nervous system phenotype of Klc^8ex94

(Gunawardena and Goldstein, 2001)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The organelle clogging phenotype seen in mutant larvae (Driven by Scer\GAL4^Appl.G1a) is suppressed by the addition of Df(3L)GN24. The organelle clogging phenotype seen in mutant larvae (Driven by Scer\GAL4^Appl.G1a) is suppressed by the addition of robl^K/+. The apoptosis phenotype seen in Hsap\APP^{695SPA4CT.Scer\UAS.T:Hsap\MYC}, Scer\GAL4^Appl.G1a animals is enhanced by the addition of Khc⁸ or Klc^8ex94. The apoptosis phenotype seen in Hsap\APP^{695SPA4CT.Scer\UAS.T:Hsap\MYC}, Scer\GAL4^Appl.G1a animals is enhanced by the addition of robl^K or Df(3L)GN24.

(Gunawardena and Goldstein, 2001)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

6702

w^*; P{UAS-APP.695SPA4CT.T:Myc}XX

Notes on Origin

Discoverer

Comments

Carried in a plasmid and transfected into SL-2 cells with a Scer\GAL4 expression plasmid. The expression and metabolism of Hsap\APP^{695SPA4CT.Scer\UAS.T:Hsap\MYC} protein in these cells has been studied.

(Fossgreen et al., 1998)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Hsap\APP^{695SPA4CT.Scer\UAS.T:Hsap\MYC}

Hsap\APP^{695SPA4CT.UAS.T:Myc}

Hsap\APP^{695SPA4CT.UAS.Tag:MYC}

Name Synonyms

Secondary FlyBase IDs

References (6)

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