FlyBase Allele Report: Dmel\Tip60[UAS.cLa]

General Information

Symbol

Dmel\Tip60^UAS.cLa

Species

D. melanogaster

Name

FlyBase ID

FBal0281767

Feature type

allele

Associated gene

Dmel\Tip60

Associated Insertion(s)

Carried in Construct

P{UAS-Tip60.L}

Also Known As

UAS-dTip60^WT, dTip60^WT, UAS-Tip60, UAS-Tip60^WT

Key Links

Transgenic product class

wild_type

(Lorbeck et al., 2011)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Lorbeck et al., 2011)

Mutagen

in vitro construct

(Lorbeck et al., 2011)

Progenitor genotype

Carried in construct

P{UAS-Tip60.L}

Cytology

Description

UAS regulatory sequences drive expression of wild-type full-length Tip60 ORF sequences.

(Lorbeck et al., 2011)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Tip60

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Alzheimer's disease

modeled by Hsap\APP^{695.UAS.Tag:MYC}

(Xu et al., 2016, Xu et al., 2014, Johnson et al., 2013, Pirooznia et al., 2012)

ameliorates Alzheimer's disease 1

modeled by Hsap\APP^{Aβ42.UAS.cBa}

(Zhang et al., 2020)

ameliorates hematologic cancer

modeled by hop^Tum

(Bailetti et al., 2019)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal memory | adult stage, with Scer\GAL4^ey-OK107

(Xu et al., 2014)

abnormal neuroanatomy | adult stage, with Scer\GAL4^ey-OK107

(Xu et al., 2016)

abnormal sleep | adult stage, with Scer\GAL4^P2.4.Pdf

(Pirooznia et al., 2012)

viable, with Scer\GAL4^337Y

(Lorbeck et al., 2011)

viable, with Scer\GAL4^Act5C.PI

(Lorbeck et al., 2011)

Phenotype Manifest In

adult mushroom body, with Scer\GAL4^ey-OK107

(Xu et al., 2016)

Detailed Description

Statement

Reference

Flies expressing Tip60^Scer\UAS.cLa under the control of Scer\GAL4^ey-OK107 show a significant reduction in total mushroom body size as compared to wild type. These flies also show a loss of mushroom body neuroadaptive response to environmental enrichment in the first 5 days of adulthood, in contrast to wild type flies, which exhibit an increase in mushroom body size when housed with other flies during the first 5 days of adulthood, versus those kept in isolation.

(Xu et al., 2016)

Scer\GAL4^ey-OK107>Tip60^Scer\UAS.cLa male flies show normal learning in a conditioned courtship suppression assay (placed with a mated female for 60 minutes), with a significant drop in the courtship index when comparing the first 10 minutes to the final 10 minutes (similar to wild type). Two minutes after training, flies are placed with a virgin female and courtship is assessed for 10 minutes: trained controls show significantly reduced courtship index when compared to sham (untrained males), whereas Scer\GAL4^ey-OK107>Tip60^Scer\UAS.cLa males show no significant drop (immediate-recall memory defect).

Mushroom bodies in Scer\GAL4^ey-OK107>Tip60^Scer\UAS.cLa third instar larvae or adults do not have significant morphological defects.

(Xu et al., 2014)

Expression of Tip60^Scer\UAS.cLa under the control of Scer\GAL4^P2.4.Pdf has no significant effect on the axonal pattern of the s-LNv neurons either in third instar larval or in adult brains. It has a weak effect on nighttime sleep in adults: the overall total amount of sleep is slightly decreased due to shorter sleep bout duration, daytime sleep is not affected.

(Pirooznia et al., 2012)

Expression of Tip60^Scer\UAS.cLa at 25[o]C from late embryonic development to adulthood, under the control of Scer\GAL4^337Y does not affect viability or generate a noticeable phenotype.

(Lorbeck et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^P2.4.Pdf, Tip60^UAS.cLa has abnormal sleep | adult stage phenotype, suppressible by Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^P2.4.Pdf

(Pirooznia et al., 2012)

NOT suppressed by

Statement

Reference

Scer\GAL4^P2.4.Pdf, Tip60^UAS.cLa has abnormal sleep | adult stage phenotype, non-suppressible by Hsap\APP^{695ΔCT.UAS.Tag:MYC}, Scer\GAL4^P2.4.Pdf

(Pirooznia et al., 2012)

Suppressor of

Statement

Reference

Tip60^UAS.cLa, Scer\GAL4^elav-C155 is a suppressor of increased cell death | adult stage phenotype of Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^elav-C155

(Zhang et al., 2020)

Tip60^UAS.cLa, Scer\GAL4^elav-C155 is a suppressor of abnormal learning | third instar larval stage phenotype of Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^elav-C155

(Zhang et al., 2020)

Tip60^UAS.cLa, Scer\GAL4^elav-C155 is a suppressor of abnormal short-term memory | third instar larval stage phenotype of Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^elav-C155

(Zhang et al., 2020)

Tip60^UAS.cLa, Scer\GAL4^elav-C155 is a suppressor of abnormal short-term memory | adult stage phenotype of Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^elav-C155

(Zhang et al., 2020)

Tip60^UAS.cLa, Scer\GAL4^elav-C155 is a suppressor of abnormal locomotor behavior | larval stage phenotype of Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^elav-C155

(Zhang et al., 2020)

Tip60^UAS.cLa, Scer\GAL4^elav-C155 is a suppressor of abnormal locomotor behavior | adult stage phenotype of Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^elav-C155

(Zhang et al., 2020)

Tip60^UAS.cLa, Scer\GAL4^elav-C155 is a suppressor of short lived phenotype of Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^elav-C155

(Zhang et al., 2020)

Tip60^UAS.cLa, Scer\GAL4^ey-OK107 is a suppressor of abnormal neuroanatomy | adult stage phenotype of Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^ey-OK107

(Xu et al., 2016)

Tip60^UAS.cLa, Scer\GAL4^ey-OK107 is a suppressor of abnormal learning | adult stage phenotype of Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^ey-OK107

(Xu et al., 2014)

Tip60^UAS.cLa, Scer\GAL4^ey-OK107 is a suppressor of abnormal memory | adult stage phenotype of Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^ey-OK107

(Xu et al., 2014)

Scer\GAL4^elav-C155/Tip60^UAS.cLa is a suppressor | partially of abnormal locomotor behavior | third instar larval stage phenotype of Scer\GAL4^elav-C155, Tip60^E431Q.UAS

(Johnson et al., 2013)

Tip60^UAS.cLa, Scer\GAL4^P2.4.Pdf is a suppressor of abnormal sleep | adult stage phenotype of Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^P2.4.Pdf

(Pirooznia et al., 2012)

Other

Statement

Reference

Hsap\APP^{695ΔCT.UAS.Tag:MYC}, Scer\GAL4^ey-OK107, Tip60^UAS.cLa has abnormal memory | adult stage phenotype

(Xu et al., 2014)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^P2.4.Pdf, Tip60^UAS.cLa has abnormal neuroanatomy | adult stage phenotype

(Pirooznia et al., 2012)

Phenotype Manifest In

Suppressor of

Statement

Reference

Tip60^UAS.cLa, Scer\GAL4^elav-C155 is a suppressor of adult Kenyon cell phenotype of Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^elav-C155

(Zhang et al., 2020)

Tip60^UAS.cLa, Scer\GAL4^elav-C155 is a suppressor of mushroom body phenotype of Hsap\APP^{Aβ42.UAS.cBa}, Scer\GAL4^elav-C155

(Zhang et al., 2020)

Tip60^UAS.cLa, Scer\GAL4^ey-OK107 is a suppressor of adult mushroom body phenotype of Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^ey-OK107

(Xu et al., 2016)

Other

Statement

Reference

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^P2.4.Pdf, Tip60^UAS.cLa has axon | adult stage phenotype

(Pirooznia et al., 2012)

Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^P2.4.Pdf, Tip60^UAS.cLa has s-LNv Pdf neuron | adult stage phenotype

(Pirooznia et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Co-expression of Tip60^Scer\UAS.cLa suppresses the mushroom body morphological defects, observed in flies expressing Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^ey-OK107. The mushroom body neuroadaptive response to environmental enrichment is also restored in these flies.

(Xu et al., 2016)

Co-expression of Tip60^Scer\UAS.cLa (but not Tip60^{E431Q.Scer\UAS}) suppresses learning and memory defects in Scer\GAL4^ey-OK107>Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} males. Co-expression of Tip60^Scer\UAS.cLa or Tip60^{E431Q.Scer\UAS} does not affect learning but abolishes short-term memory of courtship suppression in Scer\GAL4^ey-OK107>Hsap\APP^{695ΔCT.Scer\UAS.T:Hsap\MYC} males.

(Xu et al., 2014)

Co-expression of Tip60^Scer\UAS.cLa partially suppresses the locomotor defects and vesicle accumulation on motor axons that is seen in third instar larvae expressing Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^elav-C155.

(Johnson et al., 2013)

Simultaneous expression of Tip60^Scer\UAS.cLa with Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^P2.4.Pdf has no effect on the axonal pattern of the s-LNv neurons in third instar larval brain, while it leads to a large increase in the area of the s-LNv neuron axonal arbor in the adult brain and it also rescues the nighttime sleep defects observed in adult flies expressing either of the transgenes alone. Co-expression with Hsap\APP^{695ΔCT.Scer\UAS.T:Hsap\MYC} has no significant effect on the axonal pattern of the s-LNv neurons either in larval or in adult brains and it does not rescue but rather enhance the nighttime sleep deficit observed in flies expressing Tip60^Scer\UAS.cLa alone.

(Pirooznia et al., 2012)

Complementation and Rescue Data

Rescues

Scer\GAL4^elav-C155/Tip60^UAS.cLa rescues Tip60^E431Q.UAS

(Johnson et al., 2013)

Tip60^UAS.cLa/Scer\GAL4^P2.4.Pdf rescues Tip60^E431Q.UAS

(Pirooznia et al., 2012)

Scer\GAL4^337Y/Tip60^UAS.cLa rescues Tip60^E431Q.UAS

(Lorbeck et al., 2011)

Comments

Co-expression of Tip60^Scer\UAS.cLa rescues the locomotor behaviour defects seen in third instar larvae expressing Tip60^{E431Q.Scer\UAS} under the control of Scer\GAL4^elav-C155.

(Johnson et al., 2013)

The loss of s-LNv terminal synaptic arborization and reduced axon length in the adult brain as well as the sleep defects characteristic for flies expressing Tip60^{E431Q.Scer\UAS} under the control of Scer\GAL4^P2.4.Pdf is rescued by co-expression of Tip60^Scer\UAS.cLa.

(Pirooznia et al., 2012)

Co-expression of Tip60^Scer\UAS.cLa rescues the lethality associated with the expression of Tip60^{E431Q.Scer\UAS}, when both transgenes are under the control of Scer\GAL4^337Y.

(Lorbeck et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Tip60^Scer\UAS.cLa

Tip60^UAS.cLa

dTip60^WT

(Johnson et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (9)

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