Deletion of the Dsp1 open reading frame (no other transcription units are affected).
A 4327bp deletion resulting from the imprecise excision of P{}Dsp1274. 17 bases of P element sequences remain at the site of the excision as well at least 3.4kb of unknown sequence.
0.7% of hemizygous males show transformation of haltere into wing. 8% of these males show transformation of abdominal segment A6 into A5.
Dsp11 flies show a homeotic first to third leg transformation at a low frequency.
Homozygous adults do not show paralysis after vibration of the culture vial. Homozygotes and hemizygotes die prematurely, show very low fertility and have various homeotic transformations. The first type of transformation corresponds to a T1 to T2 transformation; hemizygous males have reduced sex combs, with an average of 6 teeth (compared to the 11 seen in wild type). The size of the sex comb never exceeds 9 teeth in these males and is always reduced, whether or not the mother is homozygous or heterozygous for Dsp11. The second type of transformation is a T3 to a T2 transformation; partial transformations of metathoracic into mesothoracic structures are seen, mostly in the anterior compartment. The transformation may include, to various extents, dorsal development of wing tissue in place of haltere (generally only 1 haltere is affected) or mesonotal tissue in place of metanotum. The third type of transformation corresponds to an A6 to A5 transformation; about 25% of hemizygous males show bristles on the A6 sternite, some having more than 6 bristles. In addition, approximately 50% of males have patches of pigmentation on the A4 tergite, suggesting a partial transformation of A4 into A5.
Homozygotes are poorly fertile and only 50% of eggs lead to viable larvae. Embryos which do not give rise to larvae stop development before the cellular blastoderm stage.
Few hemizygous males survive to adulthood. They have severe morphological and physiological defects; they walk with difficulty, can hardly fly and have severely impaired fertility. These flies die prematurely (50% die within 5 days of eclosion). Few homozygous females survive to adulthood. They have the same phenotypical characteristics as hemizygous males. The females achieve complete oogenesis but lay few eggs. At 26oC these eggs do not hatch into viable larvae. At 21oC approximately 50% of eggs give rise to viable larvae. Eggs that do not give rise to larvae that are derived from either heterozygous or homozygous females either look like eggs derived from virgin females or progress past the postblastoderm stage and show developmental arrest at any stage during embryogenesis. Some of these embryos show complex and variable cuticular defects, including in most cases loss or fusion of the A4-A5 denticle belts.
Dsp11, HUAS.cMa, Scer\GAL4GMR.PF has lethal phenotype
Dsp11 has haltere phenotype, enhanceable by Ubx[+]/Ubxbx-83Kb
Dsp11 has wing | ectopic phenotype, enhanceable by Ubx[+]/Ubxbx-83Kb
Dsp11 has abdominal sternite bristle | ectopic phenotype, enhanceable by Df(3R)P9/+
Dsp11 has metathoracic leg | ectopic phenotype, non-enhanceable by Df(2R)ED3921
Dsp11 has prothoracic leg phenotype, non-enhanceable by Df(2R)ED3921
Dsp11 is a suppressor of mesothoracic leg phenotype of ScrS
Dsp11 is a suppressor of metathoracic leg phenotype of ScrS
Dsp11 is a suppressor of mesothoracic leg phenotype of Pc11
Dsp11 is a suppressor of metathoracic leg phenotype of Pc11
Dsp11, Rm62[+]/Rm6201086 has adult abdominal segment 6 phenotype
The Df(2R)ED3921 deficiency does not enhance the homeotic leg transformation of Dsp11 flies; the wing phenotype of the deficiency is likewise unaffected. No genetic interaction is observed in the triple mutant Dsp11; Df(2R)ED3921; Df(3R)Exel6191/+.
Dsp11/Y ; Scr4/+ males show an increase in the severity of the phenotype; the size of the sex comb teeth is greatly reduced, with an average number of 4 teeth in the double mutant versus either single mutant combination. Dsp11/Y ; ScrS/+ males have a less severe phenotype than ScrS/+ single mutants; the number of T3 legs with a sex comb is highly diminished (30 vs 100%) and the size of the sex comb on the T2 legs is reduced (2.5 vs 4.5 teeth). The frequency of transformation of halteres into wings seen in Dsp11/Y flies is enhanced by one copy of Ubxbx-34e, Ubxbx-83Kb, trxE2 or ash122. The enhancement is dramatically reduced if the female parents are heterozygous rather than homozygous for Dsp11. flies is enhanced by one copy of trx1. About 90% of Dsp11/Y ; Df(3R)P9/+ males have bristles on the A6 sternite, compared to 25% in Dsp11 or Df(3R)P9 single mutants. The number of transformations of antennae into legs is reduced in Dsp11/+ ; AntpD43/+ animals. flies is enhanced by trx1/+, trxE2/+ or ash122/+. brm2/+, ash21/+ or ash218/+ does not significantly enhance the frequency of transformation of halteres into wings seen in Dsp11/Y flies. The number of T2 and T3 legs with sex combs is reduced in Dsp11/Y ; Pc11/+ flies compared to Pc11/+ single mutants. The size of the sex comb on the first leg is almost normal in Dsp11/Y ; Pc11/+ flies.
Dsp11 is rescued by Dsp1UAS.cDa/Scer\GAL4hs.PB
Complements: bss1. Dsp11 complements the bang sensitive paralysis of bss1; the phenotype of bss1/Dsp11 flies is the same as that of +/bss1 flies.