FlyBase Allele Report: Dmel\gbb[D20]

General Information

Symbol

Dmel\gbb^D20

Species

D. melanogaster

Name

FlyBase ID

FBal0090533

Feature type

allele

Associated gene

Dmel\gbb

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl nitrosourea

Nature of the Allele

Allele class

Mutagen

ethyl nitrosourea

(Chen et al., 1998)

Progenitor genotype

Cytology

Description

The premature stop codon falls in the prodomain.

(Chen et al., 1998)

Amino acid replacement: Q137term.

(Chen et al., 1998)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:23,852,163..23,852,163 [+]

Nucleotide change:

C23852163T

Amino acid change:

Q137term | gbb-PA; Q137term | gbb-PB

Reported amino acid change:

Q137term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Chen et al., 1998)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal (with Df(2R)bw-S46)

(Fritsch et al., 2012, Fritsch et al., 2010)

lethal (with gbb⁴)

(Fritsch et al., 2010)

visible | somatic clone

(Fritsch et al., 2010)

Phenotype Manifest In

female germline stem cell | heat sensitive (with gbb⁴)

(Song et al., 2004)

male germline stem cell | heat sensitive (with gbb⁴)

(Kawase et al., 2004)

posterior crossvein (with gbb⁴)

(Fritsch et al., 2010)

wing blade anterior compartment | somatic clone

(Fritsch et al., 2010)

wing vein L5 (with gbb⁴)

(Fritsch et al., 2010)

wing vein L5 | somatic clone

(Fritsch et al., 2010)

Detailed Description

Statement

Reference

Homozygous clones in the anterior compartment of the wing result in wings which are smaller than normal and which show truncation of vein L5 at the junction with the posterior crossvein.

Rare gbb⁴/gbb^D20 adult escapers have small wings that lack the posterior crossvein and the distal quarter of vein L5.

(Fritsch et al., 2010)

In gbb⁴/gbb^D20 males raised to adulthood at 18^oC and then shifted to 22^oC for 7 days, testes contain pn average 1.3 germline stem cells compared to an average of 9.1 in wild-type. Germline stem cells are eliminated completely after a week at 25^oC.

(Kawase et al., 2004)

In gbb⁴/gbb^D20 females raised to adulthood at 18^oC and then shifted to 29^oC for 7 days, 60% of germaria lack germ cells. Remaining germaria mostly contain only one germ cell.

(Song et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

gbb^D20/Df(2R)bw-S46 has lethal phenotype, suppressible | partially by Hsap\BMP7^gbb.PF

(Fritsch et al., 2012)

gbb^D20/Df(2R)bw-S46 has lethal phenotype, suppressible by gbb::Hsap\BMP7^BMP7LD.gbb

(Fritsch et al., 2012)

gbb^D20/Df(2R)bw-S46 has lethal phenotype, suppressible by Agam\gbb2^gbb.PF

(Fritsch et al., 2010)

gbb^D20 has visible | somatic clone phenotype, suppressible by scw^gbb.PF

(Fritsch et al., 2010)

gbb^D20/gbb⁴ has lethal phenotype, suppressible | partially by scw^gbb.PF

(Fritsch et al., 2010)

gbb^D20/Df(2R)bw-S46 has lethal phenotype, suppressible | partially by scw^gbb.PF

(Fritsch et al., 2010)

gbb^D20/Df(2R)bw-S46 has lethal phenotype, suppressible by Dgri\gbb^+tFa

(Fritsch et al., 2010)

gbb^D20/Df(2R)bw-S46 has lethal phenotype, suppressible by Agam\gbb1^gbb.PF

(Fritsch et al., 2010)

NOT suppressed by

Statement

Reference

gbb^D20/Df(2R)bw-S46 has lethal phenotype, non-suppressible by Hsap\BMP7^R292G.gbb

(Fritsch et al., 2012)

NOT Enhancer of

Statement

Reference

gbb^D20 is a non-enhancer of increased cell number | adult stage phenotype of mir-958^KO

(Mukherjee et al., 2021)

NOT Suppressor of

Statement

Reference

gbb^D20 is a non-suppressor of increased cell number | adult stage phenotype of mir-958^KO

(Mukherjee et al., 2021)

Other

Statement

Reference

gbb^D20, myd[+]/myd^3PM71 has abnormal flight phenotype

(Sidisky et al., 2021)

gbb^D20, tkv⁶ has visible | dominant phenotype

(Chen et al., 1998)

gbb^D20, tkv⁶ has visible | recessive phenotype

(Chen et al., 1998)

gbb^D20, put¹³⁵ has visible | dominant phenotype

(Chen et al., 1998)

dpp^s5, gbb^D20 has visible | dominant phenotype

(Chen et al., 1998)

Df(2L)tkv2, gbb^D20 has visible | dominant phenotype

(Chen et al., 1998)

gbb^D20, shn¹ has visible | dominant phenotype

(Chen et al., 1998)

Phenotype Manifest In

Suppressed by

Statement

Reference

gbb^D20 has posterior crossvein | somatic clone phenotype, suppressible by scw^gbb.PF

(Fritsch et al., 2010)

gbb^D20 has wing vein L5 | somatic clone phenotype, suppressible by scw^gbb.PF

(Fritsch et al., 2010)

gbb^D20 has wing blade anterior compartment | somatic clone phenotype, suppressible by scw^gbb.PF

(Fritsch et al., 2010)

gbb^D20 has wing blade posterior compartment | somatic clone phenotype, suppressible by scw^gbb.PF

(Fritsch et al., 2010)

gbb^D20/gbb⁴ has posterior crossvein phenotype, suppressible by scw^gbb.PF

(Fritsch et al., 2010)

gbb^D20/gbb⁴ has wing vein L5 phenotype, suppressible by scw^gbb.PF

(Fritsch et al., 2010)

gbb^D20/gbb⁴ has male germline stem cell | heat sensitive phenotype, suppressible | partially by Dad[+]/Dad^P1883

(Kawase et al., 2004)

NOT Enhancer of

Statement

Reference

gbb^D20 is a non-enhancer of intestinal stem cell | adult stage | increased number phenotype of mir-958^KO

(Mukherjee et al., 2021)

NOT Suppressor of

Statement

Reference

gbb^D20 is a non-suppressor of intestinal stem cell | increased number | adult stage phenotype of mir-958^KO

(Mukherjee et al., 2021)

Other

Statement

Reference

bam^hs.PO, gbb^D20/gbb⁴ has follicle cell & germarium | conditional - heat sensitive phenotype

(Kirilly et al., 2005)

bam^hs.PO, gbb^D20/gbb⁴ has germarium | heat sensitive phenotype

(Kirilly et al., 2005)

gbb^D20, tkv⁶ has scutum phenotype

(Chen et al., 1998)

gbb^D20, tkv⁶ has leg phenotype

(Chen et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

myd^3PM71/+, gbb^D20/+ double heterozygotes show a decrease in flight ability.

(Sidisky et al., 2021)

The reduced wing size and vein L5 truncation seen in flies carrying homozygous gbb^D20 clones in the anterior compartment of the wing are suppressed by scw^gbb.PF; the wing is nearly normal in size and vein L5 reaches the wing margin.

The loss of the posterior crossvein and loss of the distal tip of vein L5 which are seen in flies carrying homozygous gbb^D20 clones in the posterior compartment of the wing are both partially suppressed by a single copy of scw^gbb.PF, resulting in a small fragment of posterior crossvein which is detached from both the L4 and L5 veins and an almost complete L5 vein.

A single copy of scw^gbb.PF partially rescues the lethality of gbb⁴/gbb^D20 animals (34% of the expected number of adults are recovered) and the loss of posterior crossvein and truncation of L5 that are seen in gbb⁴/gbb^D20 adult escapers are partially restored. Two copies of scw^gbb.PF rescue the posterior crossvein and vein L5 phenotypes to nearly wild type.

scw^gbb.PF rescues the lethality of gbb^D20/Df(2R)bw-S46 animals; lethality is rescued from the larval/pupal stage to the pharate adult, with rare viable escapers.

(Fritsch et al., 2010)

bam^hs.PO animals cultured at 18^oC and heat shocked as late stage pupae (37^oC, 4 times for 2 hours each with an 8-12 hour interval) produce adult females with agametic germaria. If these animals also carry the temperature sensitive allelic combination gbb⁴/gbb^D20 and are shifted to 29^oC (the non-permissive temperature) for 10 days, no follicle cells remain, although the resulting germaria do contain terminal filament cells.

(Kirilly et al., 2005)

gbb⁴/gbb^D20 males raised to adulthood at 18^oC and then shifted to 22^oC for 4 days have an average of 2.4 germline stem cells per testis. This is increased to 4.8 by the presence of Dad^P1883/+.

(Kawase et al., 2004)

gbb^D20 dominantly enhances the tkv⁶ phenotype; tkv⁶ homozygotes have a normal notum and legs, but the presence of one copy of gbb^D20 results in defects in the notum and legs. Double heterozygotes with Df(2L)tkv2, dpp^s5, shn¹ or put¹³⁵ may show imaginal disc development defects. No interaction with sax⁵, Mad^P1 or Med⁴ is seen in double heterozygous flies.

(Chen et al., 1998)

Xenogenetic Interactions

Statement

Reference

Dgri\gbb^+tFa, Agam\gbb1^gbb.PF and Agam\gbb2^gbb.PF each completely rescue the lethality of gbb^D20/Df(2R)bw-S46 animals.

(Fritsch et al., 2010)

Complementation and Rescue Data

Rescued by

gbb^D20/Df(2R)bw-S46 is rescued by gbb^WT.Tag:FLAG

(Fritsch et al., 2012)

gbb^D20/Df(2R)bw-S46 is rescued by gbb^{R325A.Tag:FLAG}

(Fritsch et al., 2012)

gbb^D20/Df(2R)bw-S46 is rescued by gbb^{K334N.Tag:FLAG}

(Fritsch et al., 2012)

gbb^D20/Df(2R)bw-S46 is rescued by gbb^{R126A.Tag:FLAG}

(Fritsch et al., 2012)

gbb^D20/Df(2R)bw-S46 is rescued by gbb^{R325A.K334N.Tag:FLAG}

(Fritsch et al., 2012)

gbb^D20/Df(2R)bw-S46 is rescued by gbb^+t3

(Fritsch et al., 2010)

Not rescued by

gbb^D20/Df(2R)bw-S46 is not rescued by gbb^{R126A.R325A.Tag:FLAG}

(Fritsch et al., 2012)

gbb^D20/Df(2R)bw-S46 is not rescued by gbb^{R126A.R325A.K334N.Tag:FLAG}

(Fritsch et al., 2012)

gbb^D20/Df(2R)bw-S46 is not rescued by gbb^scw.PF

(Fritsch et al., 2010)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

63054

y¹ w^*; P{FRT(w^hs)}G13 sha¹ gbb^D20/SM6a

Notes on Origin

Discoverer

Selected as: Dominant enhancer of the tkv⁶ phenotype. Induced on: tkv⁶.

(Chen et al., 1998)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

D20

(Chen et al., 1998)

Gbb^D20

(Sidisky et al., 2021)

Tgfβ-60A^D20

gbb^D20

(Mukherjee et al., 2021, Galeone et al., 2017, Liu et al., 2014, Fritsch et al., 2012, Fritsch et al., 2010, O'Connor-Giles et al., 2008, Kirilly et al., 2005)

Name Synonyms

Secondary FlyBase IDs

References (11)

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