FlyBase Allele Report: Dmel\myd[3PM71]

General Information

Symbol

Dmel\myd^3PM71

Species

D. melanogaster

Name

FlyBase ID

FBal0367562

Feature type

allele

Associated gene

Dmel\myd

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Sidisky et al., 2021)

Mutagen

Progenitor genotype

Cytology

Description

A deletion and an amino acid change within the second exon of myd.

(Sidisky et al., 2021)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:19,190,239..19,190,244 [+]

Comment:

A 6bp deletion within the first coding exon of myd. One of two mutations in myd in myd^3PM71.

(Sidisky et al., 2021)

point_mutation

3R:19,190,547..19,190,547 [+]

Nucleotide change:

G19190547T

Amino acid change:

E156D | myd-PA; E156D | myd-PB

Comment:

A missense mutation within the first coding exon of mdy. One of two mutations in myd in myd^3PM71.

(Sidisky et al., 2021)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult neuromuscular junction

(Sidisky et al., 2021)

dorsal longitudinal indirect flight muscle cell

(Sidisky et al., 2021)

NMJ bouton | adult stage | decreased number

(Sidisky et al., 2021)

Detailed Description

Statement

Reference

myd^3PM71 dominantly leads to a progressive decrease in flight ability, with myd^3PM71/Df(3R)ED5938, myd^3PM71/myd^MB03509 and myd^3PM71/myd^MI08666 transheterozygotes showing a similar progressive decrease in flight ability.

myd^3PM71 mutants DLM neurons show increased apoptosis (TUNEL-positive cell bodies in the adult thoracic ganglia) neuromuscular junctions show progressive decreases in branch number and length, as well as in bouton number. Their nuclear

myd^3PM71/myd^3PM71 mutants expressing myd^UAS.Venus under the control of either Scer\GAL4^αTub84B.PL alone, or Scer\GAL4^Mhc.PK and Scer\GAL4^VGlut-OK371 combined show DLM neuromuscular junctions with increased branch number and length, as well as increased bouton number. myd^3PM71/myd^3PM71 mutants expressing myd^UAS.Venus under the control of Scer\GAL4^Mhc.PK alone show DLM neuromuscular junctions with increased branch number and length.

(Sidisky et al., 2021)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

myd^3PM71 has abnormal flight phenotype, suppressible by tkv^CA.UAS/Scer\GAL4^VGlut-OK371

(Sidisky et al., 2021)

myd^3PM71 has abnormal neuroanatomy | adult stage | progressive phenotype, suppressible | partially by Hsap\SDF4^UAS.cSa/Scer\GAL4^αTub84B.PL

(Sidisky et al., 2021)

myd^3PM71 has abnormal neuroanatomy | adult stage | progressive phenotype, suppressible | partially by Scer\GAL4^Mhc.PK/Scer\GAL4^VGlut-OK371/Hsap\SDF4^UAS.cSa

(Sidisky et al., 2021)

myd^3PM71 has abnormal neuroanatomy | adult stage | progressive phenotype, suppressible | partially by Scer\GAL4^Mhc.PK/Hsap\SDF4^UAS.cSa

(Sidisky et al., 2021)

myd^3PM71 has abnormal flight | dominant | progressive phenotype, suppressible | partially by Hsap\SDF4^UAS.cSa/Scer\GAL4^αTub84B.PL

(Sidisky et al., 2021)

myd^3PM71 has abnormal flight | dominant | progressive phenotype, suppressible | partially by Scer\GAL4^Mhc.PK/Scer\GAL4^VGlut-OK371/Hsap\SDF4^UAS.cSa

(Sidisky et al., 2021)

NOT suppressed by

Statement

Reference

myd^3PM71 has abnormal flight | dominant | progressive phenotype, non-suppressible by Scer\GAL4^Mhc.PK/Hsap\SDF4^UAS.cSa

(Sidisky et al., 2021)

Other

Statement

Reference

Mad^1-2, myd[+]/myd^3PM71 has abnormal flight phenotype

(Sidisky et al., 2021)

gbb^D20, myd[+]/myd^3PM71 has abnormal flight phenotype

(Sidisky et al., 2021)

myd[+]/myd^3PM71, tkv⁷ has abnormal flight phenotype

(Sidisky et al., 2021)

myd[+]/myd^3PM71, tkv⁸ has abnormal flight phenotype

(Sidisky et al., 2021)

myd[+]/myd^3PM71, wit^B11 has abnormal flight phenotype

(Sidisky et al., 2021)

Hsap\SDF4^UAS.cSa, Scer\GAL4^αTub84B.PL, myd^3PM71 has abnormal neuroanatomy | adult stage phenotype

(Sidisky et al., 2021)

Hsap\SDF4^UAS.cSa, Scer\GAL4^Mhc.PK, Scer\GAL4^VGlut-OK371, myd^3PM71 has abnormal neuroanatomy | adult stage phenotype

(Sidisky et al., 2021)

Hsap\SDF4^UAS.cSa, Scer\GAL4^Mhc.PK, myd^3PM71 has abnormal neuroanatomy | adult stage phenotype

(Sidisky et al., 2021)

Phenotype Manifest In

Suppressed by

Statement

Reference

myd^3PM71 has adult neuromuscular junction phenotype, suppressible | partially by Scer\GAL4^Mhc.PK/Scer\GAL4^VGlut-OK371/Hsap\SDF4^UAS.cSa

(Sidisky et al., 2021)

myd^3PM71 has NMJ bouton | adult stage | decreased number phenotype, suppressible | partially by Scer\GAL4^Mhc.PK/Scer\GAL4^VGlut-OK371/Hsap\SDF4^UAS.cSa

(Sidisky et al., 2021)

myd^3PM71 has adult neuromuscular junction phenotype, suppressible | partially by Hsap\SDF4^UAS.cSa/Scer\GAL4^αTub84B.PL

(Sidisky et al., 2021)

myd^3PM71 has adult neuromuscular junction phenotype, suppressible | partially by Scer\GAL4^Mhc.PK/Hsap\SDF4^UAS.cSa

(Sidisky et al., 2021)

myd^3PM71 has NMJ bouton | adult stage | decreased number phenotype, suppressible | partially by Hsap\SDF4^UAS.cSa/Scer\GAL4^αTub84B.PL

(Sidisky et al., 2021)

myd^3PM71 has NMJ bouton | adult stage | decreased number phenotype, suppressible | partially by Scer\GAL4^Mhc.PK/Hsap\SDF4^UAS.cSa

(Sidisky et al., 2021)

Additional Comments

Genetic Interactions

Statement

Reference

The following double heterozygotes show a decrease in flight ability: myd^3PM71/+, wit^B11/+; myd^3PM71/+, gbb^D20/+; myd^3PM71/+, tkv⁷/+; myd^3PM71/+, tkv⁸/+; myd^3PM71/+, Mad^1-2/+.

(Sidisky et al., 2021)

Xenogenetic Interactions

Statement

Reference

myd^3PM71/myd^3PM71 mutants expressing Hsap\SDF4^UAS.cSa under the control of either Scer\GAL4^αTub84B.PL or Scer\GAL4^Mhc.PK and Scer\GAL4^VGlut-OK371 combined show DLM neuromuscular junctions with increased branch number and length, as well as increased bouton number.

(Sidisky et al., 2021)

Complementation and Rescue Data

Rescued by

myd^3PM71 is rescued by myd^UAS.Venus/Scer\GAL4^Mhc.PK/Scer\GAL4^VGlut-OK371

(Sidisky et al., 2021)

myd^3PM71 is rescued by myd^UAS.Venus/Scer\GAL4^αTub84B.PL

(Sidisky et al., 2021)

Partially rescued by

myd^3PM71 is partially rescued by myd^UAS.Venus/Scer\GAL4^Mhc.PK

(Sidisky et al., 2021)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Myd^3PM71

(Jetti et al., 2023)

myd^3PM71

(Sidisky et al., 2021)

Name Synonyms

Secondary FlyBase IDs

References (2)

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