FlyBase Allele Report: Dmel\MRG15[j6A3]

General Information

Symbol

Dmel\MRG15^j6A3

Species

D. melanogaster

Name

FlyBase ID

FBal0086983

Feature type

allele

Associated gene

Dmel\MRG15

Associated Insertion(s)

P{lacW}MRG15^j6A3

Carried in Construct

Also Known As

l(3)j6A3

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(BDGP Project Members, 1994-1999)

Progenitor genotype

Associated Insertion(s)

P{lacW}MRG15^j6A3

Cytology

Description

Insertion in the second exon, 174 nucleotides downstream of the ATG codon.

(Qi et al., 2006)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:15,277,800..15,277,800 [+]

(Joyce et al., 2011, Qi et al., 2006, Zraly et al., 2002, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Zraly et al., 2002, Spradling et al., 1999, BDGP Project Members, 1994-1999)

suppressor of variegation | dominant, with T(1;4)w^m258-21

(Qi et al., 2006)

Phenotype Manifest In

Detailed Description

Statement

Reference

MRG15^j6A3 mutant germline cells exhibit a complete absence of His2Av, both phosphorylated and unphosphorylated, throughout oogenesis.

(Joyce et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

MRG15^j6A3 is an enhancer of visible phenotype of Scer\GAL4^bbg-C96, dom^RNAi.Sym.UAS

(Kwon et al., 2013)

Other

Statement

Reference

MRG15^j6A3, Pc[+]/Pc¹¹ has visible | dominant phenotype

(Qi et al., 2006)

MRG15^j6A3, Psc[+]/Psc¹ has visible | dominant phenotype

(Qi et al., 2006)

Phenotype Manifest In

Enhancer of

Statement

Reference

MRG15^j6A3 is an enhancer of wing margin phenotype of Scer\GAL4^bbg-C96, dom^RNAi.Sym.UAS

(Kwon et al., 2013)

Other

Statement

Reference

MRG15^j6A3, Pc[+]/Pc¹¹ has mesothoracic leg & sex comb | ectopic phenotype

(Qi et al., 2006)

MRG15^j6A3, Pc[+]/Pc¹¹ has metathoracic leg & sex comb | ectopic phenotype

(Qi et al., 2006)

MRG15^j6A3, Psc[+]/Psc¹ has mesothoracic leg & sex comb | ectopic phenotype

(Qi et al., 2006)

MRG15^j6A3, Psc[+]/Psc¹ has metathoracic leg & sex comb | ectopic phenotype

(Qi et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

The wing nicking phenotype caused by expression of dom^{dsRNA.Sym.Scer\UAS} under the control of Scer\GAL4^bbg-C96 is enhanced if the flies are also carrying MRG15^j6A3.

(Kwon et al., 2013)

MRG15^j6A3 Pc¹¹ double heterozygous males have ectopic sex combs on the second and third legs (85% and 54% of flies respectively).

Psc¹/+ ; MRG15^j6A3/+ double heterozygous males have ectopic sex combs on the second and third legs (38% and 11% of flies respectively).

MRG15^j6A3 does not show an ectopic sex comb phenotype in either the second or third legs in double heterozygous combination with esc¹.

(Qi et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (3)

Bloomington

10290

y¹ w¹¹¹⁸; P{lacW}MRG15^j6A3/TM3, Sb¹

59966

y² w¹ ct⁶; Df(3R)E(mod)4-1^P11, P{lacW}MRG15^j6A3/TM6B, Tb¹

Kyoto

111041

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{neoFRT}82B P{lacW}MRG15^j6A3/TM6B, P{Car20y}TPN1, Tb¹

Notes on Origin

Discoverer

Separable from: a second site lethal mutation on the same chromosome.

(Qi et al., 2006)

Comments

Complements: Hsc70-4⁰³⁵⁵⁰. Complements: Hsc70-4^j1C1.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

MRG15^P{lacW}PJ6A3

(Pai et al., 2004)

MRG15^j6A3

(Kwon et al., 2013, Joyce et al., 2011, Cook et al., 2009.2.28)

dMRG15^P

(Qi et al., 2006)

dMRG15^j6A3

(Qi et al., 2006)

l(3)j6A3

(Zraly et al., 2002, Spradling et al., 1999)

l(3)j6A3^j6A3

(BDGP Project Members, 1994-1999)

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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