From: 	kercook@XXXX
	Subject: 	Isolation and characterization of Df(3R)BSC741
	Date: 	28 February 2009  17:34:51  GMT
Isolation and characterization of Df(3R)BSC741
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC741 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}Setf03083 and P{XP}CG6218d06481. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; P{hs-hid}3, Dr1/TM6C, Sb1 cu1 females crossed to P{hsFLP}1, y1 w1118; PBac{WH}Setf03083/P{XP}CG6218d06481 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC741 from the segment of PBac{WH}Setf03083 to the left of its FRT site and the segment of P{XP}CG6218d06481 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(3R)BSC741 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are 88E8;88F1. Df(3R)BSC741 failed to complement ea1 and MRG15j6A3.