FlyBase Allele Report: Dmel\drl[UAS.cCa]

General Information

Symbol

Dmel\drl^UAS.cCa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Callahan

FlyBase ID

FBal0057584

Feature type

allele

Associated gene

Dmel\drl

Associated Insertion(s)

Carried in Construct

P{UAS-drl}

Also Known As

UAS-drl

Key Links

Transgenic product class

cDNA

(Callahan et al., 1996)

wild_type

(Callahan et al., 1996)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Callahan et al., 1996)

wild_type

(Callahan et al., 1996)

Mutagen

in vitro construct

(Callahan et al., 1996)

Progenitor genotype

Carried in construct

P{UAS-drl}

Cytology

Description

UASt regulatory sequences drive expression of a 3.1kb EcoRI fragment of drl cDNA.

(Callahan et al., 1996)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

drl

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates intellectual disability

modeled by Ube3a^unspecified

(Chakraborty et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^ap-md52

(Bonkowsky et al., 1999)

abnormal neurophysiology, with Scer\GAL4^eg-Mz360

(Yoshikawa et al., 2003)

Phenotype Manifest In

embryonic ventral nerve cord, with Scer\GAL4^ap-md52

(Bonkowsky et al., 1999)

interneuron, with Scer\GAL4^eg-Mz360

(Yoshikawa et al., 2003)

larval posterior commissure, with Scer\GAL4^eg-Mz360

(Yoshikawa et al., 2001, Bonkowsky et al., 1999)

Detailed Description

Statement

Reference

Pan-neural expression of drl^Scer\UAS.cCa, under the control of Scer\GAL4^elav-C155 induces the partial or complete loss of adult mushroom body orthogonal lobes. The severity of this gain-of-function phenotype is dosage-dependent since the percentage of mushroom bodies affected increases with the number of copies of the drl^Scer\UAS.cCa transgene. The complete absence of lobes is only seen when four copies of the drl^Scer\UAS.cCa transgene are present.

(Taillebourg et al., 2005)

When drl^Scer\UAS.cCa is driven by Scer\GAL4^eg-Mz360 expressing interneurons switch their projections to the anterior commissure in all segments, compared to one cluster projecting to the anterior and one to the posterior commissure.

(Yoshikawa et al., 2003)

When expression is driven by Scer\GAL4^7B no deleterious effect on learning scores can be detected.

(Moreau-Fauvarque et al., 2002)

Expression of three copies of drl^Scer\UAS.cCa under the control of Scer\GAL4^eg-Mz360 results in all the eg-expressing posterior commissure axons switching their projections and crossing the midline in the anterior commissure, forming a bundle distinct from the normal anterior commissure bundle.

(Yoshikawa et al., 2001)

Expression of drl^Scer\UAS.cCa under the control of Scer\GAL4^eg-Mz360 in embryos has no effect on the eg-expressing neurons that normally cross the midline in the anterior commissure. However, the eg-expressing neurons which normally cross the midline in the posterior commissure switch their projections to the AC in all segments. This phenotype is dose-dependent. Expression of drl^Scer\UAS.cCa under the control of Scer\GAL4^ap-md52 causes the growth cones of ap-expressing neurons to stall as they approach the level of the posterior commissure, but the neurons remain ipsilateral as in wild-type embryos.

(Bonkowsky et al., 1999)

Scer\GAL4^D82 induced expression of P{UAS-drl} in drl^R343 embryos partially rescues the 'bypass' phenotype.

(Callahan et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^eg-Mz360, drl^UAS.cCa has abnormal neurophysiology phenotype, suppressible by Wnt5^D7

(Yoshikawa et al., 2003)

Suppressor of

Statement

Reference

drl^UAS.cCa/Scer\GAL4^elav.PU is a suppressor of abnormal learning | dominant | maternal effect phenotype of Ube3a^unspecified

(Chakraborty et al., 2015)

Other

Statement

Reference

Scer\GAL4^ap-md52, comm^UAS.cKa, drl^UAS.cCa has abnormal neuroanatomy phenotype

(Bonkowsky et al., 1999)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Scer\GAL4^eg-Mz360, drl^UAS.cCa has interneuron phenotype, non-enhanceable by Wnt4^EMS23

(Yoshikawa et al., 2003)

Scer\GAL4^eg-Mz360, drl^UAS.cCa has interneuron phenotype, non-enhanceable by dsh³

(Yoshikawa et al., 2003)

Scer\GAL4^eg-Mz360, drl^UAS.cCa has interneuron phenotype, non-enhanceable by fz¹⁵/fz2^C1

(Yoshikawa et al., 2003)

Scer\GAL4^eg-Mz360, drl^UAS.cCa has interneuron phenotype, non-enhanceable by wg^unspecified

(Yoshikawa et al., 2003)

Suppressed by

Statement

Reference

Scer\GAL4^eg-Mz360, drl^UAS.cCa has interneuron phenotype, suppressible by Wnt5^D7

(Yoshikawa et al., 2003)

NOT suppressed by

Statement

Reference

Scer\GAL4^eg-Mz360, drl^UAS.cCa has interneuron phenotype, non-suppressible by Wnt4^EMS23

(Yoshikawa et al., 2003)

Scer\GAL4^eg-Mz360, drl^UAS.cCa has interneuron phenotype, non-suppressible by dsh³

(Yoshikawa et al., 2003)

Scer\GAL4^eg-Mz360, drl^UAS.cCa has interneuron phenotype, non-suppressible by fz¹⁵/fz2^C1

(Yoshikawa et al., 2003)

Scer\GAL4^eg-Mz360, drl^UAS.cCa has interneuron phenotype, non-suppressible by wg^unspecified

(Yoshikawa et al., 2003)

Other

Statement

Reference

Scer\GAL4^ap-md52, comm^UAS.cKa, drl^UAS.cCa has embryonic ventral nervous system & embryonic thoracic segment phenotype

(Bonkowsky et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of drl^Scer\UAS.cCa under the control of Scer\GAL4^elav.PU rescues the learning defects seen in flies which have a maternally inherited null mutation of Ube3a.

(Chakraborty et al., 2015)

The interneuron phenotype seen in drl^Scer\UAS.cCa, Scer\GAL4^eg-Mz360 animals is suppressed by the addition of Wnt5^D7. The addition of wg^unspecified, Wnt4^EMS23, or dsh³ has no effect on the interneuron phenotype seen in drl^Scer\UAS.cCa, Scer\GAL4^eg-Mz360 animals. The addition of fz¹⁵ and fz2^C1 has no effect on the interneuron phenotype seen in drl^Scer\UAS.cCa, Scer\GAL4^eg-Mz360 animals.

(Yoshikawa et al., 2003)

Expression of comm^Scer\UAS.cKa under the control of Scer\GAL4^ap-md52 causes ap-expressing neurons to cross the midline, in contrast to wild type. Thoracic-specific ap-expressing neurons cross in the posterior commissure (PC), whereas other ap-expressing neurons cross in the anterior commissure (AC). Co-expression of drl^Scer\UAS.cCa switches the projections of all thoracic-specific ap-expressing neurons from the PC to the AC.

(Bonkowsky et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

drl^UAS.cCa/Scer\GAL4^Mef2.PR rescues drl^Red2

(Lahaye et al., 2012)

drl^UAS.cCa/Scer\GAL4^fkh.PZ rescues drl^R343

(Harris and Beckendorf, 2007)

drl^UAS.cCa/Scer\GAL4^elav-C155 rescues drl²

(Taillebourg et al., 2005)

drl^UAS.cCa/Scer\GAL4^elav-C155 rescues drl^R343

(Taillebourg et al., 2005)

drl^UAS.cCa/Scer\GAL4^7B rescues drl^P1

(Moreau-Fauvarque et al., 2002)

drl^UAS.cCa/Scer\GAL4^7B rescues drl^P1

(Moreau-Fauvarque et al., 1998)

Partially rescues

drl^UAS.cCa/Scer\GAL4^7B partially rescues drl^exc21

(Moreau-Fauvarque et al., 2002)

drl^UAS.cCa/Scer\GAL4^7B partially rescues drl²

(Moreau-Fauvarque et al., 2002)

drl^UAS.cCa/Scer\GAL4^7B partially rescues drl²/drl^R343

(Moreau-Fauvarque et al., 1998)

drl^UAS.cCa/Scer\GAL4^c739 partially rescues drl²/drl^R343

(Moreau-Fauvarque et al., 1998)

drl^UAS.cCa/Scer\GAL4^D82 partially rescues drl^R343

(Callahan et al., 1996)

Fails to rescue

drl^UAS.cCa/Scer\GAL4^sr-md710 fails to rescue drl^Red2

(Lahaye et al., 2012)

drl^UAS.cCa/Scer\GAL4^GH146 fails to rescue drl^R343

(Sakurai et al., 2009)

drl^UAS.cCa/Scer\GAL4^7B fails to rescue drl^P1

(Moreau-Fauvarque et al., 2002)

Comments

Expression of drl^Scer\UAS.cCa under the control of Scer\GAL4^Mef2.PR rescues the lateral transverse muscle attachment defects seen in drl^Red2 mutant stage 16 embryos. No rescue is seen when drl^Scer\UAS.cCa is expressed under the control of Scer\GAL4^sr-md710.

(Lahaye et al., 2012)

Expression of drl^Scer\UAS.cCa driven by Scer\GAL4^GH146 fails to restore the DA1 position phenotype found in drl^R343 mutant brains.

(Sakurai et al., 2009)

The salivary gland guidance defects of drl^R343 embryos are fully rescued by expression of drl^Scer\UAS.cCa under the control of Scer\GAL4^fkh.PZ.

(Harris and Beckendorf, 2007)

Pan-neural expression of two copies of drl^Scer\UAS.cCa under the control of Scer\GAL4^elav-C155 rescues the phenotype of drl²/drl^R343, with 68% of the mushroom bodies reverting to a wild-type phenotype. Pan-neural expression of drl^Scer\UAS.cCa, under the control of Scer\GAL4^elav-C155 in results in 16% of the mushroom bodies remaining mutant and 16% displaying a phenotype identical to the gain-of-function phenotype. Pan-neural expression of drl^Scer\UAS.cCa, under the control of Scer\GAL4^elav-C155 in drl²/drl^R343 mutants results in 16% of the mushroom bodies remaining mutant and 16% displaying a phenotype identical to the gain-of-function phenotype.

(Taillebourg et al., 2005)

Expression driven by Scer\GAL4^7B rescues mushroom body phenotype of drl^P1, partially rescues the mushroom body defect of drl² and the learning defect of drl² and drl^exc21, but does not rescue the learning phenotype of drl^P1.

(Moreau-Fauvarque et al., 2002)

The mushroom body and central complex defects of drl²/drl^R343 flies are almost completely rescued by drl^Scer\UAS.cCa expressed under the control of Scer\GAL4^7B. The mushroom body and central complex defects of drl²/drl^R343 flies are partially rescued by drl^Scer\UAS.cCa expressed under the control of Scer\GAL4^c739. The mushroom body and central complex defects of drl^P1/drl^P1 flies are completely rescued by drl^Scer\UAS.cCa expressed under the control of Scer\GAL4^7B.

(Moreau-Fauvarque et al., 1998)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

drl^Scer\UAS.cCa

drl^UAS.cCa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Callahan

(Callahan et al., 1996)

Secondary FlyBase IDs

References (12)

Report Sections

Open Close