FlyBase Allele Report: Dmel\comm[UAS.cKa]

General Information

Symbol

Dmel\comm^UAS.cKa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Kidd

FlyBase ID

FBal0086681

Feature type

allele

Associated gene

Dmel\comm

Associated Insertion(s)

Carried in Construct

P{UAS-comm.K}

Also Known As

UAS-comm

Key Links

Transgenic product class

cDNA

(Kidd et al., 1998)

wild_type

(Kidd et al., 1998)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Kidd et al., 1998)

wild_type

(Kidd et al., 1998)

Mutagen

in vitro construct

(Kidd et al., 1998)

Progenitor genotype

Carried in construct

P{UAS-comm.K}

Cytology

Description

A 1.7kb XhoI-XbaI comm cDNA fragment is expressed under the control of UASt regulatory sequences.

(Kidd et al., 1998)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

comm

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^ap-md52

(Bonkowsky et al., 1999)

abnormal neuroanatomy, with Scer\GAL4^eve.CQ2

(Mauss et al., 2009)

abnormal neuroanatomy, with Scer\GAL4^shot-OK307

(Godenschwege et al., 2002)

abnormal neuroanatomy | embryonic stage, with Scer\GAL4^elav-C155

(van den Brink et al., 2013)

Phenotype Manifest In

adult antennal lobe commissure, with Scer\GAL4^SG18.1

(Jhaveri et al., 2004)

antennal lobe glomerulus, with Scer\GAL4^SG18.1

(Jhaveri et al., 2004)

chordotonal organ & embryo & nerve terminal, with Scer\GAL4^12.1

(Zlatic et al., 2003)

dendrite, with Scer\GAL4^eve.CQ2

(Mauss et al., 2009)

embryonic/larval neuron, with Scer\GAL4^sca-537.4

(Kidd et al., 1998)

embryonic ventral nerve cord, with Scer\GAL4^ap-md52

(Bonkowsky et al., 1999)

ganglionic tracheal branch primordium, with Scer\GAL4^btl.PS

(Englund et al., 2002)

giant fiber neuron, with Scer\GAL4^shot-OK307

(Godenschwege et al., 2002)

larval longitudinal connective | embryonic stage, with Scer\GAL4^elav-C155

(van den Brink et al., 2013)

motor neuron, with Scer\GAL4^eve.CQ2

(Mauss et al., 2009)

pCC neuron, with Scer\GAL4^sca-537.4

(Kidd et al., 1998)

presumptive embryonic/larval central nervous system, with Scer\GAL4^sca-537.4

(Kidd et al., 1999)

Detailed Description

Statement

Reference

The expression of comm^Scer\UAS.cKa under the control of Scer\GAL4^Pdf.PU does not significantly affect the length of sLNv axons compared to controls.

(Oliva et al., 2016)

Expression of comm^Scer\UAS.cKa under the control of Scer\GAL4^elav-C155 results in a "roundabout" phenotype of longitudinal axons crossing the midline in embryos.

(van den Brink et al., 2013)

Down-regulation of robo through targeted expression of comm^Scer\UAS.cKa under the control of Scer\GAL4^eve.CQ2 induces ectopic dendritic innervation of the midline neuropile (ith 100% penetrance for MN-LL1 and 62.5% penetrance for MN-DA3) with a concomitant reduction of the lateral arbor.

(Mauss et al., 2009)

The antennal lobes of comm^Scer\UAS.cKa; Scer\GAL4^SG18.1 flies have a disorganized pattern of glomeruli, and while the antennal commissure is present, its axons are loosely fasciculated.

(Jhaveri et al., 2004)

When expression is driven by Scer\GAL4^12.1 the cd sensory neuron projections are shifted from the intermediate to the medial longitudinal fascicles. In approximately 50% of cases, cd sensory neuron projections cross the midline.

(Zlatic et al., 2003)

Longitudinal tracts look normal in embryos expressing comm^Scer\UAS.cKa under the control of Scer\GAL4^btl.PS. 17% of ganglionic branches cross the midline in these embryos, 14% turn anteriorly at the midline and 15% stall prematurely.

(Englund et al., 2002)

Expression of comm^Scer\UAS.cKa under the regulation of Scer\GAL4^A307, in the giant fiber system results in the collapse of the giant fiber axons at the midline, with the axons often wrapping around each other. The latency (a measure of synaptic function) of these axons is unaffected. Expression of comm^Scer\UAS.cKa in the tergotrochanteral motor neurons, under the control of Scer\GAL4^{shakB.lethal.4.1} has no effect on the dendritic projection or the function of the synapse.

(Godenschwege et al., 2002)

Expression of comm^Scer\UAS.cKa under the control of Scer\GAL4^ap-md52 causes ap-expressing neurons to cross the midline, in contrast to wild type. Thoracic-specific ap-expressing neurons cross in the posterior commissure (PC), whereas other ap-expressing neurons cross in the anterior commissure (AC).

(Bonkowsky et al., 1999)

Axons freely cross and recross the midline in the central nervous system (CNS) of embryos expressing comm^Scer\UAS.cKa under the control of Scer\GAL4^sca-537.4. Increasing the copy number of comm^Scer\UAS.cKa results in a more severe phenotype, the strongest phenotype has axons entering the midline but not leaving it, leading to a collapse of the CNS scaffold onto the midline.

(Kidd et al., 1999)

More axons than normal cross the midline in embryos expressing comm^Scer\UAS.cKa under the control of Scer\GAL4^sca-537.4. The pCC pathway crosses the midline, joins with its contralateral pathway and forms circles around the midline. The midline glia are present and migrate to their normal position. The phenotype caused by comm^Scer\UAS.cKa expressed under the control of Scer\GAL4^sca-537.4 is dosage sensitive.

(Kidd et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Scer\GAL4^elav-C155, comm^UAS.cKa has abnormal neuroanatomy | embryonic stage phenotype, non-suppressible by Rab5^{S43N.UASp.YFP}, Scer\GAL4^elav-C155

(van den Brink et al., 2013)

Suppressor of

Statement

Reference

Scer\GAL4^eg-Mz360/comm^UAS.cKa is a suppressor of abnormal neuroanatomy | recessive | embryonic stage phenotype of fra^unspecified

(Yang et al., 2009)

comm^UAS.cKa/Scer\GAL4^shot-OK307 is a suppressor of abnormal neuroanatomy phenotype of Scer\GAL4^shot-OK307, robo2^UAS.cSa

(Godenschwege et al., 2002)

Scer\GAL4^{shakB.lethal.4.1}/comm^UAS.cKa is a suppressor | partially of abnormal neuroanatomy phenotype of Scer\GAL4^{shakB.lethal.4.1}, robo1^UAS.cKa

(Godenschwege et al., 2002)

Scer\GAL4^{shakB.lethal.4.1}/comm^UAS.cKa is a suppressor of abnormal neurophysiology phenotype of Scer\GAL4^{shakB.lethal.4.1}, robo1^UAS.cKa

(Godenschwege et al., 2002)

comm^UAS.cKa/Scer\GAL4^shot-OK307 is a suppressor of abnormal neuroanatomy phenotype of Scer\GAL4^shot-OK307, robo1^UAS.cKa

(Godenschwege et al., 2002)

Other

Statement

Reference

Df(1)NetAB^Δ, Scer\GAL4^eg-Mz360, comm^UAS.cKa has abnormal neuroanatomy | embryonic stage phenotype

(Yang et al., 2009)

Scer\GAL4^ap-md52, comm^UAS.cKa, drl^UAS.cCa has abnormal neuroanatomy phenotype

(Bonkowsky et al., 1999)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Scer\GAL4^elav-C155, comm^UAS.cKa has larval longitudinal connective | embryonic stage phenotype, non-suppressible by Rab5^{S43N.UASp.YFP}, Scer\GAL4^elav-C155

(van den Brink et al., 2013)

Suppressor of

Statement

Reference

Scer\GAL4^eg-Mz360/comm^UAS.cKa is a suppressor of larval EW neuron phenotype of fra^unspecified

(Yang et al., 2009)

comm^UAS.cKa/Scer\GAL4^shot-OK307 is a suppressor of giant fiber neuron phenotype of Scer\GAL4^shot-OK307, robo2^UAS.cSa

(Godenschwege et al., 2002)

comm^UAS.cKa/Scer\GAL4^elav.PLu is a suppressor of symmetrical commissure phenotype of Scer\GAL4^elav.PLu, fra::robo1^{UAS.FR.Tag:MYC}

(Bashaw and Goodman, 1999)

Other

Statement

Reference

Df(1)NetAB^Δ, Scer\GAL4^eg-Mz360, comm^UAS.cKa has larval EW neuron phenotype

(Yang et al., 2009)

Scer\GAL4^ap-md52, comm^UAS.cKa, drl^UAS.cCa has embryonic ventral nervous system & embryonic thoracic segment phenotype

(Bonkowsky et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Rab5^{S43N.Scer\UAS.P\T.T:Avic\GFP-YFP} has no effect on the "roundabout" phenotype (longitudinal axons crossing the midline) in embryos expressing comm^Scer\UAS.cKa under the control of Scer\GAL4^elav-C155.

(van den Brink et al., 2013)

Expression of comm^Scer\UAS.cKa under the control of Scer\GAL4^eg-Mz360 partially suppresses the EW axon guidance defects in fra^unspecified mutants.

Overexpression of comm^Scer\UAS.cKa under the control of Scer\GAL4^eg-Mz360 in Df(1)NetAB^Δ mutants does not affect the guidance defects seen in Df(1)NetAB^Δ mutant embryos.

(Yang et al., 2009)

The lateral shifts in the giant fibers induced by overexpression of robo^Scer\UAS.cKa or lea^Scer\UAS.cSa, under the control of Scer\GAL4^A307 can be suppressed by co-overexpression of comm^Scer\UAS.cKa with Scer\GAL4^A307. When comm^Scer\UAS.cKa is coexpressed with robo^Scer\UAS.cKa or lea^Scer\UAS.cSa, the lateral displacement of the giant fiber axons is completely rescued. When comm^Scer\UAS.cKa is coexpressed with robo^Scer\UAS.cKa, under the control of Scer\GAL4^{shakB.lethal.4.1}, one copy of comm^Scer\UAS.cKa is able to suppress the anatomical and physiological effect of one or two copies of robo^Scer\UAS.cKa.

(Godenschwege et al., 2002)

When both comm^Scer\UAS.cKa and fra::robo^{Scer\UAS.FR.T:Hsap\MYC} are both expressed in all neurons by Scer\GAL4^elav.PLu, suppression of the fra::robo^{Scer\UAS.FR.T:Hsap\MYC} phenotype is seen with some significant commissure formation in greater than 60% of segments (n+29 animals).

(Bashaw and Goodman, 1999)

Co-expression of drl^Scer\UAS.cCa under the control of Scer\GAL4^ap-md52 switches the projections of all thoracic-specific ap-expressing neurons from the PC to the AC, whereas co-expression of drl^ΔE.Scer\UAS has no effect.

(Bonkowsky et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

comm^UAS.cKa/Scer\GAL4^sim.P3.7 partially rescues comm¹

(Georgiou and Tear, 2002)

comm^UAS.cKa/Scer\GAL4^sli.PS partially rescues comm¹

(Georgiou and Tear, 2002)

Fails to rescue

comm^UAS.cKa/Scer\GAL4^sim.P3.7 fails to rescue comm^Δe39

(Georgiou and Tear, 2002)

comm^UAS.cKa/Scer\GAL4^sli.PS fails to rescue comm^Δe39

(Georgiou and Tear, 2002)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

comm^Scer\UAS.cKa

comm^UAS.cKa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Kidd

(Kidd et al., 1998)

Secondary FlyBase IDs

References (14)

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