FlyBase Allele Report: Dmel\robo2[UAS.cSa]

General Information

Symbol

Dmel\robo2^UAS.cSa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Simpson

FlyBase ID

FBal0121551

Feature type

allele

Associated gene

Dmel\robo2

Associated Insertion(s)

Carried in Construct

P{UAS-robo2.S}

Also Known As

UAS-Robo2

Key Links

Transgenic product class

wild_type

(Simpson et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Simpson et al., 2000)

Mutagen

in vitro construct

(Simpson et al., 2000)

Progenitor genotype

Carried in construct

P{UAS-robo2.S}

Cytology

Description

UAS regulatory sequences drive expression of robo2.

(Simpson et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

robo2

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^shot-OK307

(Godenschwege et al., 2002)

Phenotype Manifest In

cardioblast | ectopic, with Scer\GAL4^Mef2.PR

(Santiago-Martinez et al., 2006)

giant fiber neuron, with Scer\GAL4^shot-OK307

(Godenschwege et al., 2002)

larval longitudinal connective, with Scer\GAL4^elav.PLu

(Simpson et al., 2000)

larval ventral nerve cord, with Scer\GAL4^unspecified

(Simpson et al., 2000)

larval ventral nerve cord, with Scer\GAL4^unspecified, robo2^unspecified

(Simpson et al., 2000)

larval ventral nerve cord, with Scer\GAL4^unspecified, robo2^x135

(Simpson et al., 2000)

larval ventral nerve cord commissure, with Scer\GAL4^elav.PLu

(Simpson et al., 2000, Simpson et al., 2000)

larval ventral nerve cord commissure, with Scer\GAL4^unspecified

(Simpson et al., 2000)

larval ventral nerve cord commissure, with Scer\GAL4^unspecified, robo2^unspecified

(Simpson et al., 2000)

larval ventral nerve cord commissure, with Scer\GAL4^unspecified, robo2^x135

(Simpson et al., 2000)

motor neuron, with Scer\GAL4^unspecified, robo2^unspecified

(Simpson et al., 2000)

motor neuron, with Scer\GAL4^unspecified, robo2^x135

(Simpson et al., 2000)

muscle cell of A1-7 lateral transverse muscle 1, with Scer\GAL4^how-24B

(Kramer et al., 2001)

muscle cell of A1-7 lateral transverse muscle 2, with Scer\GAL4^how-24B

(Kramer et al., 2001)

muscle cell of A1-7 lateral transverse muscle 3, with Scer\GAL4^how-24B

(Kramer et al., 2001)

presumptive embryonic/larval central nervous system, with Scer\GAL4^unspecified

(Simpson et al., 2000)

presumptive embryonic/larval central nervous system, with Scer\GAL4^unspecified, robo2^unspecified

(Simpson et al., 2000)

presumptive embryonic/larval central nervous system, with Scer\GAL4^unspecified, robo2^x135

(Simpson et al., 2000)

Detailed Description

Statement

Reference

Expression of lea^Scer\UAS.cSa in cardioblasts using Scer\GAL4^Mef2.PR results in a lateral shift of the row of cardioblasts away from the dorsal midline to a position normally occupied by pericardial cells in approximately 84% of embryos: the distance between opposing cardioblast nuclei in these embryos (at stage 16) is approximately 22.28 μm compared to approximately 9.21 μm in stage 16 wild type embryos. This abnormal space between the cardioblast rows is maintained through stage 17 when the opposing pairs of cells should have fused at the midline. Although the cardioblast rows are displaced laterally in lea^Scer\UAS.cSa-expressing cells, there are no visible gaps between the cardioblasts, indicating that adhesion between these cells is maintained.

(Santiago-Martinez et al., 2006)

Expression of lea^Scer\UAS.cSa, in the giant fiber axons, under the control of Scer\GAL4^A307 results in a strong deflection of the fibers in the posterior regions of T1 and T2. Expression of lea^Scer\UAS.cSa, under the control of Scer\GAL4^A307 does not affect the latency of the giant fiber, with only subtle defects in following frequency. Even with two copies of lea^Scer\UAS.cSa, no physiological latency effect is found.

(Godenschwege et al., 2002)

Expression of lea^Scer\UAS.cSa under the control of Scer\GAL4^GMR.PF has no effect on retinal patterning.

(Wills et al., 2002)

Expression of lea^Scer\UAS.cSa under the control of Scer\GAL4^how-24B results in guidance defects in muscles 21 through 23, but no other significant muscle patterning defects.

(Kramer et al., 2001)

Expression of lea^Scer\UAS.cSa under the control of Scer\GAL4^elav.PLu results in a comm-like phenotype. All three Fas2-expressing longitudinal bundles are compressed into a single fascicle.

(Simpson et al., 2000)

With a low and increasing level of lea overexpression results in a inappropriate midline crossing. As levels of lea continue to increase, the response becomes biphasic. The proclivity to cross the midline is replaced at higher levels (e.g when driven by Scer\GAL4^elav.PLu) by an increased tendency to avoid the midline leading to a commissureless phenotype. The overexpression of lea^Scer\UAS.cSa in all neurons in a lea^unspecified background causes much more severe disruptions in axon pathfinding than in lea^unspecified embryos alone. CNS axons are observed leaving the CNS; some of them return into the CNS several segments later. Motor axons in the periphery cross over segment boreders and ectopically fasciculate, sometimes with axons from several segments away. The medial, intermediate, and lateral Fas2 longitudinal pathways fasciculate together and travel back and forth across the midline repeatedly. This genotype results in a more disorganised axon scaffold than does lea^Scer\UAS.cSa overexpression in a wild-type background.

(Simpson et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^{shakB.lethal.4.1}, robo2^UAS.cSa has abnormal neuroanatomy phenotype, suppressible by Scer\GAL4^{shakB.lethal.4.1}/comm^ΔC.UAS

(Godenschwege et al., 2002)

Scer\GAL4^{shakB.lethal.4.1}, robo2^UAS.cSa has abnormal neurophysiology phenotype, suppressible by Scer\GAL4^{shakB.lethal.4.1}/comm^ΔC.UAS

(Godenschwege et al., 2002)

Scer\GAL4^shot-OK307, robo2^UAS.cSa has abnormal neuroanatomy phenotype, suppressible by comm^UAS.cKa/Scer\GAL4^shot-OK307

(Godenschwege et al., 2002)

Enhancer of

Statement

Reference

robo2^UAS.cSa is an enhancer of visible phenotype of Abl^UAS.cFa, Scer\GAL4^GMR.PF

(Wills et al., 2002)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^Mef2.PR, robo2^UAS.cSa, robo2^X123 has cardioblast | ectopic phenotype, enhanceable by robo1⁴

(Santiago-Martinez et al., 2006)

NOT Enhanced by

Statement

Reference

Scer\GAL4^Mef2.PR, robo2^UAS.cSa has cardioblast | ectopic phenotype, non-enhanceable by robo1⁴

(Santiago-Martinez et al., 2006)

Suppressed by

Statement

Reference

Scer\GAL4^{shakB.lethal.4.1}, robo2^UAS.cSa has giant fiber neuron phenotype, suppressible by Scer\GAL4^{shakB.lethal.4.1}/comm^ΔC.UAS

(Godenschwege et al., 2002)

Scer\GAL4^shot-OK307, robo2^UAS.cSa has giant fiber neuron phenotype, suppressible by comm^UAS.cKa/Scer\GAL4^shot-OK307

(Godenschwege et al., 2002)

Scer\GAL4^elav.PLu, robo2^UAS.cSa has larval ventral nerve cord commissure phenotype, suppressible | partially by robo1¹

(Simpson et al., 2000)

Scer\GAL4^elav.PLu, robo2^UAS.cSa has larval ventral nerve cord commissure phenotype, suppressible | partially by robo1⁵/robo1¹

(Simpson et al., 2000)

Scer\GAL4^elav.PLu, robo2^UAS.cSa has larval ventral nerve cord commissure phenotype, suppressible | partially by sli²

(Simpson et al., 2000)

Scer\GAL4^elav.PLu, robo2^UAS.cSa has larval ventral nerve cord commissure phenotype, suppressible | partially by ena^GC1

(Simpson et al., 2000)

NOT suppressed by

Statement

Reference

Scer\GAL4^Mef2.PR, robo2^UAS.cSa has cardioblast | ectopic phenotype, non-suppressible by robo1⁴

(Santiago-Martinez et al., 2006)

Enhancer of

Statement

Reference

robo2^UAS.cSa is an enhancer of ommatidium phenotype of Abl^UAS.cFa, Scer\GAL4^GMR.PF

(Wills et al., 2002)

Other

Statement

Reference

Scer\GAL4^Mef2.PR, robo1⁴, robo2^UAS.cSa has embryo | dorsal closure stage phenotype

(Santiago-Martinez et al., 2006)

Scer\GAL4^Mef2.PR, robo1⁴, robo2^UAS.cSa, robo2^X123 has cardioblast phenotype

(Santiago-Martinez et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of lea^Scer\UAS.cSa in cardioblasts using Scer\GAL4^Mef2.PR in a robo⁴ mutant background results in similar defects as when lea^Scer\UAS.cSa is expressed using Scer\GAL4^Mef2.PR in an otherwise wild type background. Namely, a lateral shift of the row of cardioblasts away from the dorsal midline to a position normally occupied by pericardial cells while adhesion between these cells is maintained.

Embryos expressing lea^Scer\UAS.cSa under the control of Scer\GAL4^Mef2.PR in a robo⁴, lea^X123 double mutant background display gaps in rows of cardioblasts indicating a loss of adhesion between cardioblasts (as seen in robo⁴, lea^X123 double mutants) as well as the mispositioning of these cells away from the dorsal midline (as seen in lea^Scer\UAS.cSa, Scer\GAL4^Mef2.PR embryos).

Embryos expressing lea^Scer\UAS.cSa under the control of Scer\GAL4^Mef2.PR in a robo⁴, lea^X123 double mutant background exhibit mispositioning of cardioblasts away from the dorsal midline which is more severe as compared to expression of lea^Scer\UAS.cSa using Scer\GAL4^Mef2.PR in an otherwise wild type background; cardioblasts are displaced even more laterally, with cardioblasts and pericardial cells even being observed in somatic muscle territory.

Expression of lea^Scer\UAS.cSa under the control of Scer\GAL4^Mef2.PR in a robo⁴ mutant background results in a slight delay in dorsal closure: a slight gap between dorsal ectodermal cells is seen in late stage mutant embryos that is not observed in wild type embryos.

(Santiago-Martinez et al., 2006)

The lateral shifts in the giant fibers induced by overexpression of lea^Scer\UAS.cSa, under the control of Scer\GAL4^A307 can be suppressed by co-overexpression of comm^Scer\UAS.cKa with Scer\GAL4^A307. Co-expression of one copy of comm^ΔC.Scer\UAS, under the regulation of Scer\GAL4^{shakB.lethal.4.1}, is able to rescue the anatomical and physiological effect of one or two copies of lea^Scer\UAS.cSa.

(Godenschwege et al., 2002)

Co-expression of lea^Scer\UAS.cSa enhances the rough eye phenotype caused by expression of Abl^Scer\UAS.cFa under the control of Scer\GAL4^GMR.PF.

(Wills et al., 2002)

The commissureless phenotype seen when lea^Scer\UAS.cSa is driven by Scer\GAL4^elav.PLu, is partially suppressed by the addition of ena^GC1/+, robo¹/+, robo¹/robo⁵ or sli²/+. Although the number of commissure that form in these backgrounds is increased, the phenotype is more complex than simple suppression because in many cases the crossovers that now occur are inappropriate.

(Simpson et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

lea^Scer\UAS.cSa

robo2^Scer\UAS.cSa

(Kramer et al., 2001, Simpson et al., 2000)

robo2^UAS.cSa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Simpson

(Simpson et al., 2000)

Secondary FlyBase IDs

References (7)

Report Sections

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