FlyBase Allele Report: Dmel\robo2[unspecified]

General Information

Symbol

Dmel\robo2^unspecified

Species

D. melanogaster

Name

FlyBase ID

FBal0086418

Feature type

allele

Associated gene

Dmel\robo2

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy

(Grueber et al., 2007, Englund et al., 2002)

lethal - all die during embryonic stage | recessive

(Tearle and Nusslein-Volhard, 1987)

some die during embryonic stage | recessive

(Tearle and Nusslein-Volhard, 1987)

Phenotype Manifest In

Detailed Description

Statement

Reference

46% of ganglionic branches stall before reaching the midline turning point in mutant embryos, and 11% of them do not even enter the central nervous system. The longitudinal tracts are disrupted and the outer fascicles fuse with the medial fascicles.

(Englund et al., 2002)

In lea mutants no pCC or aCC growth cones stray from their normal pathways.

(Rajagopalan et al., 2000)

Fas2 staining axons in the central nervous system (CNS) exhibit clear mutant phenotypes. Some axons ectopically cross the midline. There is also disorganisation of the longitudinal tracts. This appears as "braiding". Instead of maintaining their parallel alignment the three Fas2 bundles on each side of the CNS cross over and intermittently join with each other on their own side. Segments that show misrouting of axons between bundles on the same side are more common that those that show axons crossing the midline. Con staining axons are often fused into a single group of axons. The overexpression of lea^Scer\UAS.cSa in all neurons in a lea^unspecified background causes much more severe disruptions in axon pathfinding than in lea^unspecified embryos alone. CNS axons are observed leaving the CNS; some of them return into the CNS several segments later. Motor axons in the periphery cross over segment boreders and ectopically fasciculate, sometimes with axons from several segments away. The medial, intermediate, and lateral Fas2 longitudinal pathways fasciculate together and travel back and forth across the midline repeatedly. This genotype results in a more disorganised axon scaffold than does lea^Scer\UAS.cSa overexpression in a wild-type background.

(Simpson et al., 2000)

lea^unspecified mutant embryos show incomplete head involution. Abdominal transformations occur with Pc like mutants.

(Tearle and Nusslein-Volhard, 1987)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

robo2^unspecified is a non-enhancer of abnormal neuroanatomy | embryonic stage phenotype of mud³

(Cate et al., 2016)

Phenotype Manifest In

Enhanced by

Statement

Reference

robo2^unspecified has presumptive embryonic/larval central nervous system phenotype, enhanceable by robo[+]/robo1^unspecified

(Simpson et al., 2000)

robo2^unspecified has larval longitudinal connective phenotype, enhanceable by robo[+]/robo1^unspecified

(Simpson et al., 2000)

robo2^unspecified has larval ventral nerve cord phenotype, enhanceable by robo[+]/robo1^unspecified

(Simpson et al., 2000)

robo2^unspecified has presumptive embryonic/larval central nervous system phenotype, enhanceable by robo1^unspecified/robo1^unspecified

(Simpson et al., 2000)

robo2^unspecified has larval longitudinal connective phenotype, enhanceable by robo1^unspecified/robo1^unspecified

(Simpson et al., 2000)

robo2^unspecified has larval ventral nerve cord phenotype, enhanceable by robo1^unspecified/robo1^unspecified

(Simpson et al., 2000)

NOT Enhanced by

Statement

Reference

robo1^unspecified, robo2^unspecified has presumptive embryonic/larval central nervous system phenotype, non-enhanceable by comm^Δe39

(Simpson et al., 2000)

robo1^unspecified, robo2^unspecified has larval ventral nerve cord phenotype, non-enhanceable by comm^Δe39

(Simpson et al., 2000)

robo1^unspecified, robo2^unspecified has larval ventral nerve cord commissure phenotype, non-enhanceable by comm^Δe39

(Simpson et al., 2000)

robo1^unspecified, robo2^unspecified has larval longitudinal connective phenotype, non-enhanceable by comm^Δe39

(Simpson et al., 2000)

NOT suppressed by

Statement

Reference

robo1^unspecified, robo2^unspecified has presumptive embryonic/larval central nervous system phenotype, non-suppressible by comm^Δe39

(Simpson et al., 2000)

robo1^unspecified, robo2^unspecified has larval ventral nerve cord phenotype, non-suppressible by comm^Δe39

(Simpson et al., 2000)

robo1^unspecified, robo2^unspecified has larval ventral nerve cord commissure phenotype, non-suppressible by comm^Δe39

(Simpson et al., 2000)

robo1^unspecified, robo2^unspecified has larval longitudinal connective phenotype, non-suppressible by comm^Δe39

(Simpson et al., 2000)

Enhancer of

Statement

Reference

lea[+]/robo2^unspecified is an enhancer of presumptive embryonic/larval central nervous system phenotype of robo1^unspecified

(Simpson et al., 2000)

lea[+]/robo2^unspecified is an enhancer of larval longitudinal connective phenotype of robo1^unspecified

(Simpson et al., 2000)

lea[+]/robo2^unspecified is an enhancer of larval ventral nerve cord phenotype of robo1^unspecified

(Simpson et al., 2000)

robo2^unspecified/robo2^unspecified is an enhancer of presumptive embryonic/larval central nervous system phenotype of robo1^unspecified

(Simpson et al., 2000)

robo2^unspecified/robo2^unspecified is an enhancer of larval longitudinal connective phenotype of robo1^unspecified

(Simpson et al., 2000)

robo2^unspecified/robo2^unspecified is an enhancer of larval ventral nerve cord phenotype of robo1^unspecified

(Simpson et al., 2000)

NOT Enhancer of

Statement

Reference

robo2^unspecified is a non-enhancer of larval ventral nerve cord | embryonic stage phenotype of mud³

(Cate et al., 2016)

robo2^unspecified is a non-enhancer of larval ventral nerve cord commissure | embryonic stage phenotype of mud³

(Cate et al., 2016)

lea[+]/robo2^unspecified is a non-enhancer of ganglionic tracheal branch primordium phenotype of RhoGAP93B¹

(Lundstrom et al., 2004)

Other

Statement

Reference

robo1^unspecified, robo2^unspecified has presumptive embryonic/larval central nervous system phenotype

(Schimmelpfeng et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

robo2^unspecified does not enhance the midline crossing defects of mud³ mutant embryos.

(Cate et al., 2016)

21% of ganglionic branches stall outside the central nervous system in robo^unspecified lea^unspecified double mutant embryos, 31% cross the midline and 45% are misrouted. The longitudinal axons collapse along the midline.

(Englund et al., 2002)

lea^unspecified ; robo^unspecified double mutant embryos show an almost complete collapse of axon fascicles at the central nervous system midline.

(Schimmelpfeng et al., 2001)

Injection of robo3^cSa and robo3^a.cSa as dsRNA into lea^unspecified embryos results in embryos with a single Fas2-expressing longitudinal fascicle on each side of the midline. The fascicle is thicker than normal as it contains axons that would normally form the intermediate and lateral fascicles. The fascicle does not cross the midline. A small lateral Fas2-expressing bundle is sometimes seen.

(Simpson et al., 2000)

Embryos homozygous mutant for robo^unspecified and lea^unspecified show a compressed midline where all the axons approach the midline and cannot leave. The addition of comm^Δe39 does not effect this phenotype. Embryos heterozygous for lea^unspecified/+ and homozygous robo^unspecified, show ectopic crossing of the medial Fas2 pathway but the medial pathway collapses entirely onto the midline. Embryos heterozygous for robo^unspecified/+ and homozygous lea^unspecified much more ectopic crossing is seen than in lea^unspecified homozygotes.

(Simpson et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

lea^unspecified

robo2^unspecified

(Rajagopalan et al., 2000, Simpson et al., 2000, Simpson et al., 2000)

Name Synonyms

Secondary FlyBase IDs

FBal0121548

References (10)

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