FB2024_03 , released June 25, 2024
Allele: Dmel\RhoGAP93B1
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General Information
Symbol
Dmel\RhoGAP93B1
Species
D. melanogaster
Name
FlyBase ID
FBal0176024
Feature type
allele
Associated gene
Dmel\RhoGAP93B
Associated Insertion(s)
Carried in Construct
Also Known As
vilse1
Key Links
Allele class

loss of function allele

Mutagen
Nature of the Allele
Allele class

loss of function allele

(Lundstrom et al., 2004)
Mutagen
P-element activity
(Lundstrom et al., 2004)
Progenitor genotype
Cytology
Description

Contains a 1077bp deletion that removes most of the first exon of RhoGAP93B.

(Lundstrom et al., 2004)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Mutant animals exhibit misrouting in ganglionic branch (GB) outgrowth. 20% of the GBs migrate normally to the midline, but stall once they reach it. 14% fail to turn posteriorly; instead they extend straight forward toward the ventral midline, where most of them stall, or occasionally continue to migrate across the midline. Unlike wild-type, occasionally Fas2 positive longitudinal connectives cross the midline. Expression of RhoGAP93B (driven by Scer\GAL4bs-23.26) rescues the erroneous outgrowth of ganglionic branches straight toward and cross the midline seen in RhoGAP93B1 embryos. It also causes premature turns away from the midline. When homozygous mutant germ-line clones are created, only a few embryos are laid, all of which arrest early in embryogenesis.

      (Lundstrom et al., 2004)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhanced by
      Statement
      Reference

      RhoGAP93B1 has larval longitudinal connective phenotype, enhanceable by robo[+]/robo14

      (Lundstrom et al., 2004)

      RhoGAP93B1 has ganglionic tracheal branch primordium phenotype, enhanceable by robo[+]/robo14

      (Lundstrom et al., 2004)
      NOT Enhanced by
      Statement
      Reference

      RhoGAP93B1 has ganglionic tracheal branch primordium phenotype, non-enhanceable by lea[+]/robo2unspecified

      (Lundstrom et al., 2004)
      Suppressor of
      NOT Suppressor of
      Statement
      Reference

      RhoGAP93B1 is a non-suppressor of ganglionic tracheal branch primordium phenotype of Cdc423

      (Lundstrom et al., 2004)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      In RhoGAP93B1/RhoGAP93B1, robo4/+ embryos 9% of ganglionic branches cross the midline, as opposed to 1% in RhoGAP93B1 homozygotes alone. RhoGAP93B1 partially suppresses the ganglionic branch phenotype seen in Rac1J11, Rac2Δ embryos.

      (Lundstrom et al., 2004)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescued by

      RhoGAP93B1 is partially rescued by Scer\GAL4bs-23.26/RhoGAP93BUAS.cLa

      (Lundstrom et al., 2004)

      RhoGAP93B1 is partially rescued by Scer\GAL4btl.PS/RhoGAP93BUAS.cLa

      (Lundstrom et al., 2004)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      RhoGAP93B1
      vilse1
      (Morin-Poulard et al., 2016, Lundstrom et al., 2004)
      Name Synonyms
      Secondary FlyBase IDs
        References (2)