FlyBase Allele Report: Dmel\sax[P]

A P{lacW} element is inserted after amino acid 34 of sax-PA, just 5' to the GAC codon encoding amino acid 35 of the sax-PA open reading frame, and upstream from the ATG encoding methionine amino acid 36. The sax-PB open reading frame is predicted to remain intact.

(Twombly et al., 2009)

P element insert just downstream of the putative signal sequence, after amino acid 36, in the same orientation as the sax transcript.

(Nellen et al., 1994)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:7,807,119..7,807,121 [+]

(Twombly et al., 2009, Nellen et al., 1994)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal (with sax^1rv5)

(Twombly et al., 2009)

lethal | larval stage

(Nellen et al., 1994)

lethal | maternal effect

(Nellen et al., 1994)

partially lethal - majority die (with Df(2R)H23)

(Twombly et al., 2009)

partially lethal - majority die (with Df(2R)sax^1rv2)

(Twombly et al., 2009)

partially lethal - majority die (with Df(2R)sax-H9)

(Twombly et al., 2009)

partially lethal - majority die (with sax^1rv1)

(Twombly et al., 2009)

partially lethal - majority die (with sax³)

(Twombly et al., 2009)

partially lethal - majority die (with sax⁴)

(Twombly et al., 2009)

partially lethal - majority die (with sax⁵)

(Twombly et al., 2009)

partially lethal - majority die (with sax⁶)

(Twombly et al., 2009)

viable (with sax¹)

(Twombly et al., 2009)

viable (with sax²)

(Twombly et al., 2009)

visible

(Nellen et al., 1994)

Phenotype Manifest In

amnioserosa | maternal effect

(Nellen et al., 1994)

anterior crossvein

(Nellen et al., 1994)

embryonic midgut constriction 2

(Nellen et al., 1994)

eye

(Nellen et al., 1994)

female germline stem cell

(Xie and Spradling, 1998)

germarium

(Xie and Spradling, 1998)

ommatidium

(Nellen et al., 1994)

Detailed Description

Statement

Reference

Germaria from one week old females are smaller than wild type and contain only one or two stem cells. Older females may have none, though though cysts and egg chambers remain.

(Xie and Spradling, 1998)

Female adult escapers are less viable than their heterozygous siblings. Rare embryos from these females show a phenotype similar to but milder and more variable than those from sax¹/sax² females. Homozygous embryos are lacking the second midgut constriction. Homozygous females often lack the anterior crossvein, and the eyes are small due to reduced numbers of ommatidia.

(Nellen et al., 1994)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

dpp^hr4/dpp[+], sax^P has lethal | dominant | maternal effect phenotype

(Twombly et al., 2009)

dpp^hr56/dpp[+], sax^P has partially lethal - majority die | dominant | maternal effect phenotype

(Twombly et al., 2009)

dpp[+]/dpp^hr90, sax^P has partially lethal - majority die | dominant | maternal effect phenotype

(Twombly et al., 2009)

dpp^e87/dpp[+], sax^P has partially lethal - majority die | dominant | maternal effect phenotype

(Twombly et al., 2009)

sax^P, scw[+]/scw^E1 has lethal | dominant | maternal effect phenotype

(Twombly et al., 2009)

sax^P, scw^E2/scw[+] has lethal | dominant | maternal effect phenotype

(Twombly et al., 2009)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

When sax^P/+ females are crossed to dpp^hr4/+ males, most of the resulting dpp^hr4/+ progeny die.

When sax^P/+ females are crossed to males with any one of the dpp[hr56]/+, dpp[hr90]/+ or dpp[e87]/+ genotypes, the resulting heterozygous dpp mutant progeny show poor viability.

sax^P heterozygous mothers generate synthetic lethality when crossed to scw^E1/+ males.

sax^P heterozygous mothers generate synthetic lethality when crossed to scw^E2/+ males.

(Twombly et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Strong hypomorph.

(Horsfield et al., 1998)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

sax^P

(Schwank et al., 2011, Twombly et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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