FlyBase Allele Report: Dmel\rux[3]

General Information

Symbol

Dmel\rux³

Species

D. melanogaster

Name

FlyBase ID

FBal0035521

Feature type

allele

Associated gene

Dmel\rux

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Thomas et al., 1994)

Mutagen

ethyl methanesulfonate

(Thomas et al., 1994)

Progenitor genotype

Cytology

Description

The rux³ allele carries a frameshift mutation that encodes a protein with 21 out of frame amino acids after aa 320.

(Foley and Sprenger, 2001)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

X:6,036,025..6,036,043 [-]

Comment:

The exact nature and location of the mutation were inferred by the FlyBase curator. The mutation is reported as a frameshift mutation that encodes a truncated rux protein with 21 novel amino acids after amino acid 320 but the exact nature of the frameshift mutation was not specified. The mutation was annotated as a 19 bp deletion starting at amino acid 321, which would produce a protein consistent with the reported product.

(Foley and Sprenger, 2001)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

viable

(Foley and Sprenger, 2001)

visible | recessive

(Foley and Sprenger, 2001, Thomas et al., 1994)

Phenotype Manifest In

eye

(Foley and Sprenger, 2001, Dong et al., 1999, Dong et al., 1997, Thomas et al., 1994)

mitotic cell cycle

(Foley and Sprenger, 2001)

photoreceptor neuron

(Dong et al., 1997)

Detailed Description

Statement

Reference

The spatiotemporal pattern of mitotic domains is disrupted during Mitosis 14 of the developing embryo. Metaphase is on average 79% longer in mutants than in wild-type embryos. The other stages of mitosis are not significantly altered in mutant embryos.

(Foley and Sprenger, 2001)

Homozygotes exhibit a rough eye phenotype. All cells enter S phase and do so earlier than wild type, some cells establish a G₁ phase, albeit shorter in length.

(Dong et al., 1997)

Eyes are rough, viability reduced to 50% with respect to wild type.

(Thomas et al., 1994)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

rux³ has visible phenotype, enhanceable by Ras85D^e1B

(Thomas et al., 1994)

rux³ has visible phenotype, enhanceable by Ras85D^e2F

(Thomas et al., 1994)

rux³ has visible phenotype, enhanceable by S¹

(Thomas et al., 1994)

rux³ has visible phenotype, enhanceable by ast¹

(Thomas et al., 1994)

Suppressed by

Statement

Reference

rux³ has visible phenotype, suppressible by CycA³

(Thomas et al., 1994)

rux³ has visible phenotype, suppressible by CycA⁵

(Thomas et al., 1994)

rux³ has visible phenotype, suppressible by stg^RXT13

(Thomas et al., 1994)

rux³ has visible phenotype, suppressible by stg^AR2

(Thomas et al., 1994)

Phenotype Manifest In

Enhanced by

Statement

Reference

rux³ has eye phenotype, enhanceable by ebi^E90

(Dong et al., 1999)

rux³ has eye phenotype, enhanceable by ebi^E4

(Dong et al., 1999)

rux³ has eye phenotype, enhanceable by Ras85D^e1B

(Thomas et al., 1994)

rux³ has eye phenotype, enhanceable by Ras85D^e2F

(Thomas et al., 1994)

rux³ has eye phenotype, enhanceable by S¹

(Thomas et al., 1994)

rux³ has eye phenotype, enhanceable by ast¹

(Thomas et al., 1994)

Suppressed by

Statement

Reference

rux³ has phenotype, suppressible by swm^unspecified

(Dong et al., 1997)

rux³ has phenotype, suppressible by S(rux)2C¹

(Dong et al., 1997)

rux³ has phenotype, suppressible by S(rux)2C²

(Dong et al., 1997)

rux³ has phenotype, suppressible by S(rux)2C³

(Dong et al., 1997)

rux³ has phenotype, suppressible by stg^S(rux)3A

(Dong et al., 1997)

rux³ has phenotype, suppressible by CycA^S(rux)3B

(Dong et al., 1997)

rux³ has phenotype, suppressible by Rca1^S(rux)2A

(Dong et al., 1997)

rux³ has eye phenotype, suppressible by CycA³

(Thomas et al., 1994)

rux³ has eye phenotype, suppressible by CycA⁵

(Thomas et al., 1994)

rux³ has eye phenotype, suppressible by stg^RXT13

(Thomas et al., 1994)

rux³ has eye phenotype, suppressible by stg^AR2

(Thomas et al., 1994)

Enhancer of

Statement

Reference

rux³ is an enhancer of mitotic cell cycle phenotype of CycA^UAS.Δ170, Scer\GAL4^prd.RG1

(Foley and Sprenger, 2001)

Additional Comments

Genetic Interactions

Statement

Reference

The addition of rux³, prolongs the metaphase arrest phenotype seen in CycA^{Scer\UAS.Δ170},Scer\GAL4^prd.RG1 embryos.

(Foley and Sprenger, 2001)

The G₁ gap is restored when heterozygous for Rca1. swm, S(rux)2C, stg and CycA mutations also suppress cell cycle defects, restores a normal G₁ in the morphogenetic furrow.

(Dong et al., 1997)

Mutant eye phenotype of rux³ is dominantly enhanced by Ras85D^e2F and Ras85D^e1B, ast¹ and S¹. Removing a copy of Sos or rl has no effect on the severity of the rux³ mutant phenotype. 81% of ommatidia of rux³ are restored to wild type by CycA³ or CycA⁵. 36% of ommatidia of rux³ are restored to wild type by stg^RXT13 or stg^AR2.

(Thomas et al., 1994)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

rux³ is rescued by rux^+t6.0

(Thomas et al., 1994)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Weak rux allele.

(Dong et al., 1997)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

B57

rux³

(Tanaka-Matakatsu et al., 2007)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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