FlyBase Allele Report: Dmel\ap[rK568]

(Li et al., 2016, Bieli et al., 2015, Gohl et al., 2008, Herrero et al., 2007, Koelzer and Klein, 2006, Milan et al., 2002, Milan et al., 2001, O'Keefe and Thomas, 2001, Ghazi et al., 2000, Rauskolb et al., 1995, Blair, 1993, Diaz-Benjumea and Cohen, 1993, Williams et al., 1993, Cohen et al., 1992)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal (with ap^md544)

(Delanoue et al., 2002)

visible (with ap^md544)

(Tomoyasu et al., 2009, O'Keefe and Thomas, 2001)

visible (with ap^{P1\loxP.MCP-PRE.MM})

(Gohl et al., 2008)

visible (with ap^UGO35)

(Bieli et al., 2015)

visible | recessive

(Williams et al., 1993)

Phenotype Manifest In

coxal tergal remotor muscle cell

(Ghazi et al., 2000)

direct flight muscle cell

(Ghazi et al., 2000)

dorsal longitudinal indirect flight muscle cell

(Ghazi et al., 2000)

dorsal-ventral compartment boundary of the wing blade

(Blair et al., 1994)

dorsal-ventral compartment boundary of the wing disc (with ap^md544)

(Milan et al., 2001, O'Keefe and Thomas, 2001)

haltere

(Cohen et al., 1992)

indirect flight muscle cell

(Ghazi et al., 2000)

lateral oblique dorsal muscle cell

(Ghazi et al., 2000)

muscle cell of wing axillary muscle I1

(Ghazi et al., 2000)

muscle cell of wing axillary muscle III1

(Ghazi et al., 2000)

muscle cell of wing basalar muscle b2

(Ghazi et al., 2000)

tergopleural muscle cell

(Ghazi et al., 2000)

tergosternal muscle cell

(Ghazi et al., 2000)

wing

(Williams et al., 1993, Cohen et al., 1992)

wing (with ap^md544)

(O'Keefe and Thomas, 2001)

wing (with ap^{P1\loxP.MCP-PRE.MM})

(Gohl et al., 2008)

wing (with ap^UGO35)

(Bieli et al., 2015)

wing disc (with ap^md544)

(Milan and Cohen, 2003)

wing disc (with ap^UGO35)

(Koelzer and Klein, 2006)

wing margin (with ap^md544)

(O'Keefe and Thomas, 2001)

Detailed Description

Statement

Reference

ap^UGO35/ap^rK568 mutant wing discs have no wing pouch and the adult flies do not develop any wings.

(Bieli et al., 2015)

Wing defects are seen in ap^{loxP.MCP-PRE.MM}/ap^rK568 mutant flies, but they are significantly less severe than those seen in ap^{loxP.MCP-PRE.MM} homozygotes.

Wing defects are seen in Df(2R)DG-Mcp/ap^rK568 mutant flies, but they are less severe than those seen in Df(2R)DG-Mcp homozygotes.

Mild wing defects are seen in Df(2R)DG/ap^rK568 mutant flies, but they are less severe than those seen in Df(2R)DG homozygotes.

(Gohl et al., 2008)

The dorsal/ventral boundary fails to form correctly in ap^UGO35/ap^rK568 mutant wing discs.

(Koelzer and Klein, 2006)

The D/V compartment boundary of ap^rK568/ap^md544 wing discs is highly irregular (in contrast to wild type). The resulting adult wings are almost completely eliminated and show no evidence of a wing margin.

(O'Keefe and Thomas, 2001)

Adults show defects in the direct flight muscles. Of the group of four muscles 51-54, only one or two develop. IFMs are reduced in width at the posterior attachment site and do not undergo complete splitting. Muscle size and volume is reduced.

(Ghazi et al., 2000)

In mosaic analysis of wing clones generated in larvae at 60hrs AEL no ap^- clones were found that define the D/V boundary from the dorsal side. Despite a dorsal origin, ap^- cells behave like ventral cells with respect to the lineage restriction. Ventrally generated ap^- cells never cross into the dorsal compartment, but can define the D/V boundary from the ventral side. ap^- cells which cross to the ventral side usually retain their ap expression. Dorsal ap^- clones are commonly surrounded by regions of abnormal folding and outgrowth, including blister-like projections from the disc epithelium. This effect is especially pronounced near the hinge region. In rare cases the posterior wing blade is duplicated.

(Blair et al., 1994)

Complete loss of wing, retaining only a stump of wing hinge or an amorphous tissue mass.

(Williams et al., 1993)

Wings and halteres absent.

(Cohen et al., 1992)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

ap^rK568/ap^md544 has visible phenotype, suppressible | partially by Scer\GAL4^ap-md544/fng^UAS.cKa

(O'Keefe and Thomas, 2001)

Other

Statement

Reference

ap^rK568, ft^k07918 has lethal | recessive phenotype

(Garoia et al., 2000)

Phenotype Manifest In

Suppressed by

Statement

Reference

ap^rK568/ap^md544 has wing disc phenotype, suppressible | partially by fng^EP3082/Scer\GAL4^ap-md544

(Milan and Cohen, 2003)

ap^rK568/ap^md544 has wing disc phenotype, suppressible | partially by fng^{UAS.B.Tag:MYC}/Scer\GAL4^ap-md544

(Milan and Cohen, 2003)

ap^rK568/ap^md544 has dorsal-ventral compartment boundary of the wing disc phenotype, suppressible by fng^{UAS.B.Tag:MYC}/Scer\GAL4^ap-md544

(Milan et al., 2001)

ap^rK568/ap^md544 has wing disc phenotype, suppressible by Scer\GAL4^ap-md544/fng^UAS.cKa

(O'Keefe and Thomas, 2001)

ap^rK568/ap^md544 has wing margin phenotype, suppressible | partially by Scer\GAL4^ap-md544/fng^UAS.cKa

(O'Keefe and Thomas, 2001)

ap^rK568/ap^md544 has wing phenotype, suppressible | partially by Scer\GAL4^ap-md544/fng^UAS.cKa

(O'Keefe and Thomas, 2001)

NOT suppressed by

Statement

Reference

ap^rK568/ap^UGO35 has wing disc phenotype, non-suppressible by H^E31/H^E31

(Koelzer and Klein, 2006)

ap^rK568/ap^UGO35 has wing disc phenotype, non-suppressible by Su(H)⁸/Su(H)^del47

(Koelzer and Klein, 2006)

ap^rK568/ap^md544 has dorsal-ventral compartment boundary of the wing disc phenotype, non-suppressible by fng^EP3082/Scer\GAL4^ap-md544

(Milan et al., 2001)

Other

Statement

Reference

Scer\GAL4^ap-md544, ap^rK568/ap^md544, fng^UAS.cKa has haltere phenotype

(Tomoyasu et al., 2009)

Scer\GAL4^ap-md544, ap^rK568/ap^md544, fng^UAS.cKa has dorsal row of wing sensilla phenotype

(O'Keefe and Thomas, 2001)

Scer\GAL4^ap-md544, ap^rK568/ap^md544, fng^UAS.cKa has ventral row of wing sensilla | increased number phenotype

(O'Keefe and Thomas, 2001)

Additional Comments

Genetic Interactions

Statement

Reference

Flies expressing fng^Scer\UAS.cKa under the control of Scer\GAL4^ap-md544 in a ap^rK568/ap^md544 background have ventralised halteres. In addition, the halteres form dark-pigmented bristles that are similar to those formed at the wing margin.

(Tomoyasu et al., 2009)

ap^UGO35/ap^rK568; H^E31 and ap^UGO35/ap^rK568; Su(H)^del47/Su(H)⁸ wing discs fail to form a dorsal/ventral compartment boundary.

(Koelzer and Klein, 2006)

Expression of ap^Scer\UAS.cMa under the control of Scer\GAL4^vg.boundary partially rescues the loss of wing tissue seen in vg^null/vg^83b27 flies.

(Delanoue et al., 2002)

A sharp boundary between dorsal and ventral cells is restored in ap^rK568/ap^md544 wing discs expressing fng^Scer\UAS.cKa under the control of Scer\GAL4^ap-md544. No violations of the compartment boundary are seen. The resulting adult flies have wings of roughly normal size with clearly demarcated wing margin. The rescued wing margin is ventralised; dorsal-specific sensory bristles are completely missing along the anterior margin and cells on both sides of the margin secrete ventral bristle types. The dorsal and ventral wing surfaces are not adhered to each other, resulting in a balloon-like structure. ap^rK568/ap^md544 flies expressing fng^EP3082 under the control of Scer\GAL4^ap-md544 have wing margin defects.

(O'Keefe and Thomas, 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

ap^56f

(Gohl et al., 2008)

ap⁴

(Gohl et al., 2008)

ap^2a1

(Coelho et al., 2005)

ap^2a2

(Coelho et al., 2005)

Partially rescued by

ap^rK568/ap^UGO35 is partially rescued by ap^cDNA.E+P

(Bieli et al., 2015)

ap^rK568/ap^UGO35 is partially rescued by ap^cDNA.DV+E

(Bieli et al., 2015)

ap^rK568/ap^UGO35 is partially rescued by ap^cDNA.DV+E+P

(Bieli et al., 2015)

Not rescued by

ap^rK568/ap^UGO35 is not rescued by ap^cDNA.DV+P

(Bieli et al., 2015)

Comments

Expression of two copies of ap^cDNA.DV+E partially rescues the wing loss seen in ap^UGO35/ap^rK568 mutant flies. The wing margin fails to form.

Expression of two copies of ap^cDNA.DV+E+P partially rescues the wing loss seen in ap^UGO35/ap^rK568 mutant flies. Wing rescue is not perfect: a clear dorsoventral margin is observed.

Expression of two copies of ap^cDNA.DV+P fails to rescue the wing loss seen in ap^UGO35/ap^rK568 mutant flies.

Expression of two copies of ap^cDNA.E+P partially rescues the wing loss seen in ap^UGO35/ap^rK568 mutant flies. The wing margin fails to form.

(Bieli et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (2)

5374

101294

Notes on Origin

Discoverer

Comments

Phenotype almost as extreme as for a null allele.

(Williams et al., 1993)

Not a transcript null but resembles nulls in adult phenotype.

(Blair et al., 1994)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

ap^K568

(Williams et al., 1993)

ap^LacZ

(Delanoue et al., 2002)

ap^RK568

(Eade et al., 2012)

ap^rK568

(Li et al., 2016, Tomoyasu et al., 2009, Gohl et al., 2008, Herrero et al., 2007, Koelzer and Klein, 2006)

ap^rK

(Herrero et al., 2007)

ap^rk568

(Inami et al., 2021, Milan et al., 2001)

Name Synonyms

Secondary FlyBase IDs

FBal0030497

References (23)

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