FlyBase Allele Report: Dmel\Su(H)[del47]

General Information

Symbol

Dmel\Su(H)^del47

Species

D. melanogaster

Name

FlyBase ID

FBal0103950

Feature type

allele

Associated gene

Dmel\Su(H)

Associated Insertion(s)

Carried in Construct

Also Known As

Su(H)^Δ47, Su(H)⁰⁴⁷, Su(H)^D47, Su(H)Δ47, Su(H)⁴⁷

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Morel and Schweisguth, 2000)

Progenitor genotype

Su(H)¹²

(Morel and Schweisguth, 2000)

P{}Su(H)¹²

(Morel and Schweisguth, 2000)

Caused by aberration

Df(2L)del47

Cytology

Description

Imprecise excision of the P-element, resulting in the substitution of a 1881bp fragment (from -1145 to +737bp, relative to the Su(H) transcription start site) with 9 unrelated nucleotides. This deletion removes both Su(H) and l(2)35Bg transcribed sequences.

(Morel and Schweisguth, 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

delins

2L:15,038,351..15,040,233 [+]

Comment:

Imprecise excision of P{}Su(H)¹², resulting in the substitution of a 1881bp fragment (from -1145 to +737bp, relative to the Su(H) transcription start site) with 9 unrelated nucleotides. This deletion removes both Su(H) and CIAPIN1 transcribed sequences. Boundaries approximate; not clear what transcription start was used.

(Morel and Schweisguth, 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult ALl1 lineage neuron | somatic clone

(Lin et al., 2012)

adult antennal mechanosensory and motor center projection neuron | somatic clone

(Lin et al., 2012)

adult midgut

(Ohlstein and Spradling, 2007)

adult midgut epithelium | somatic clone

(Perdigoto et al., 2011)

adult posterior midgut epithelium | female limited | somatic clone

(Hudry et al., 2016)

adult uniglomerular antennal lobe projection neuron lPN | somatic clone | increased number

(Lin et al., 2012)

dorsal mesothoracic disc & neuron

(Koelzer and Klein, 2003)

egg chamber | somatic clone

(Lopez-Schier and St. Johnston, 2001)

eye

(Morel and Schweisguth, 2000)

eye disc | cell autonomous | somatic clone

(Firth and Baker, 2005)

eye disc | somatic clone

(Dominguez et al., 2004)

follicle cell | somatic clone

(Lopez-Schier and St. Johnston, 2001)

germarium region 1 | somatic clone

(Ward et al., 2006)

germinal vesicle | somatic clone

(Lopez-Schier and St. Johnston, 2001)

intestinal stem cell | somatic clone | increased number

(Bardin et al., 2010)

larval outer optic anlage | somatic clone

(Wang et al., 2011)

neuron & eye | somatic clone

(Li and Baker, 2001)

neuron & eye | supernumerary | somatic clone

(Li and Baker, 2001)

ommatidium

(Morel and Schweisguth, 2000)

photoreceptor cell R8 & eye disc | ectopic | somatic clone

(Fu and Baker, 2003)

photoreceptor cell R8 & eye disc | somatic clone

(Fu and Baker, 2003)

proneural cluster

(Koelzer and Klein, 2003)

sensory mother cell

(Koelzer and Klein, 2003)

type II neuroblast | somatic clone

(San-Juán and Baonza, 2011)

wing disc | somatic clone

(Sanders et al., 2009)

Detailed Description

Statement

Reference

The induction of Su(H)^del47 somatic clones leads to a severe increase in the adult posterior midgut mitotic index in females only, as compared to controls.

(Hudry et al., 2016)

Su(H)^del47 homozygous lateral antennal lobe neuroblast clones, induced at the newly-hatched larval stage, have fewer antennal mechanosensory and motor center neurite projection tracts and 3 times the number of later-born uniglomerular lateral antennal lobe projection neurons in the adult; clones have similar total numbers of cells to controls by adulthood and clone size and cell division (PH3 staining) are unaffected at 30 and 70 hours after larval hatching.

(Lin et al., 2012)

Multilayering of intestinal stem cells and enteroendocrine cells is seen in homozygous clones in the adult intestine.

(Perdigoto et al., 2011)

Su(H)^del47 mutant clones generated in type II neuroblasts are smaller than control clones. In contrast, clones that originate in type I clones are similar in size to controls.

(San-Juán and Baonza, 2011)

Homozygous clones in the outer proliferation center neuroepithelium (induced at late-first or early-second instar and analysed at late-third instar) adopt an irregular cell morphology rather than the normal columnar epithelial cell morphology in 78.6% of cases.

(Wang et al., 2011)

Homozygous intestinal stem cell (ISC) clones show an overproliferation of small ISC-like cells.

(Bardin et al., 2010)

Su(H)^del47 clones in the wing disc are relatively small and scattered over the disc, with very rugged edges. They are associated with a large number of dead cells in the basal side.

(Sanders et al., 2009)

Follicle stem cell clones homozygous for Su(H)^del47 persist in the ovariole approximately as well as control clones over a 14 day period.

(Vied and Kalderon, 2009)

Homozygous intestinal stem cell (ISC) clones in the adult midgut form tumours.

(Ohlstein and Spradling, 2007)

The dorsal/ventral boundary is established correctly in Su(H)^del47 mutant wing imaginal discs.

(Koelzer and Klein, 2006)

Su(H)^del47 mutant germline stem cells are maintained in the niche similarly to wild-type control clones. Su(H)^del47 clones of cap cells and escort stem cells result in a reduction of region 1 of the germaria.

(Ward et al., 2006)

Su(H)^del47 mutant cells do not enter the second mitotic wave.

(Firth and Baker, 2005)

Mitotic clones of cells carrying Su(H)^del47 rarely contribute to the presumptive anterior dorsal-ventral region of the eye disc.

(Dominguez et al., 2004)

R8 photoreceptor differentiation is normal or early in Su(H)^del47 single mutant clones. In addition, many ectopic R8 photoreceptors differentiate in these clones.

(Fu and Baker, 2003)

Mutant wing discs exhibit a lack of neural differentiation. The proneural clusters appear to arrest their development during an early phase of sensory organ precursor development. When mutant clones are made in the wing disc, a delay in the development of the pDC sensory organ precursors is seen.

(Koelzer and Klein, 2003)

Homozyogous somatic clones in the adult eye are neurogenic. Mutant cells also differentiate prematurely.

(Li and Baker, 2001)

Large homozygous mutant clones that presumably include the polar cell precursors lead to fusions between adjacent egg chambers. Mutant epithelial follicle cells continue to divide after stage 6. When mutant clones include the follicle cells at the posterior of the egg chamber, the germinal vesicle often fails to migrate and remains at the posterior pole.

(Lopez-Schier and St. Johnston, 2001)

Su(H)¹²/Su(H)^del47 animals have a rough eye phenotype.

(Morel and Schweisguth, 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Su(H)^del47 has increased cell death | somatic clone | third instar larval stage phenotype, suppressible by arm^{S10.UAS.Tag:MYC}/Scer\GAL4^αTub84B.PL

(Sanders et al., 2009)

Suppressor of

Statement

Reference

Su(H)^del47 is a suppressor of abnormal neuroanatomy | somatic clone | third instar larval stage phenotype of N^int.G.UAS, Scer\GAL4^αTub84B.PL

(San-Juán and Baonza, 2011)

Su(H)^del47 is a suppressor of increased cell number | somatic clone | third instar larval stage phenotype of N^int.G.UAS, Scer\GAL4^αTub84B.PL

(San-Juán and Baonza, 2011)

Su(H)[+]/Su(H)^del47 is a suppressor of visible | adult stage phenotype of Hsap\ATXN1L^UAS.Tag:FLAG

(Tong et al., 2011)

Su(H)[+]/Su(H)^del47 is a suppressor of visible | recessive phenotype of dx¹⁵²

(Fuwa et al., 2006)

Su(H)^del47 is a suppressor of increased cell number | somatic clone phenotype of Scer\GAL4^αTub84B.PP, dx^UAS.cMa

(Koelzer and Klein, 2006)

Su(H)^del47 is a suppressor of visible phenotype of H^unspecified

(Morel and Schweisguth, 2000)

Other

Statement

Reference

H^E31, Su(H)^del47 has abnormal size | somatic clone phenotype

(Bardin et al., 2010)

Su(H)^del47, ci⁹⁴ has abnormal mitotic cell cycle | somatic clone | cell autonomous phenotype

(Firth and Baker, 2005)

Sos^x122, Su(H)^del47 has abnormal mitotic cell cycle | somatic clone | cell autonomous phenotype

(Firth and Baker, 2005)

Mad^1-2, Su(H)^del47, ci⁹⁴ has abnormal mitotic cell cycle | somatic clone | cell autonomous phenotype

(Firth and Baker, 2005)

Mad^1-2, Su(H)^del47 has abnormal mitotic cell cycle | somatic clone | cell autonomous phenotype

(Firth and Baker, 2005)

Mad¹², Su(H)^del47, ci⁹⁴ has abnormal mitotic cell cycle | somatic clone | cell autonomous phenotype

(Firth and Baker, 2005)

Mad¹², Su(H)^del47 has abnormal mitotic cell cycle | somatic clone | cell autonomous phenotype

(Firth and Baker, 2005)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Su(H)^del47 has proneural cluster phenotype, non-enhanceable by Psn^C1

(Koelzer and Klein, 2003)

Suppressed by

Statement

Reference

Su(H)^del47 has photoreceptor cell R8 & eye disc | ectopic | somatic clone phenotype, suppressible by ci⁹⁴

(Fu and Baker, 2003)

NOT suppressed by

Statement

Reference

Su(H)^del47 has intestinal stem cell | increased number | somatic clone phenotype, non-suppressible by H^E31/H^E31

(Bardin et al., 2010)

Su(H)^del47 has proneural cluster phenotype, non-suppressible by Psn^C1

(Koelzer and Klein, 2003)

Enhancer of

Statement

Reference

Su(H)^del47 is an enhancer of ommatidium phenotype of aop^A141

(Rohrbaugh et al., 2002)

Suppressor of

Statement

Reference

Su(H)^del47 is a suppressor of type II neuroblast | ectopic | somatic clone | third instar larval stage phenotype of N^int.G.UAS, Scer\GAL4^αTub84B.PL

(San-Juán and Baonza, 2011)

Su(H)[+]/Su(H)^del47 is a suppressor of wing blade phenotype of Hsap\ATXN1L^UAS.Tag:FLAG

(Tong et al., 2011)

Su(H)[+]/Su(H)^del47 is a suppressor of wing vein L5 phenotype of Hsap\ATXN1L^UAS.Tag:FLAG

(Tong et al., 2011)

Su(H)[+]/Su(H)^del47 is a suppressor of posterior crossvein phenotype of Hsap\ATXN1L^UAS.Tag:FLAG

(Tong et al., 2011)

Su(H)[+]/Su(H)^del47 is a suppressor of wing vein phenotype of dx¹⁵²

(Fuwa et al., 2006)

Su(H)^del47 is a suppressor of wing disc phenotype of Psn^C1

(Koelzer and Klein, 2006)

Su(H)^del47 is a suppressor of macrochaeta phenotype of H^unspecified

(Morel and Schweisguth, 2000)

NOT Suppressor of

Statement

Reference

Su(H)⁸/Su(H)^del47 is a non-suppressor of wing disc phenotype of ap^rK568/ap^UGO35

(Koelzer and Klein, 2006)

Other

Statement

Reference

Pka-C1^B3, Su(H)^del47, hid^GMR.PG has eye phenotype

(Christiansen et al., 2013)

Scer\GAL4^αTub84B.PL, Su(H)^del47, arm^{S10.UAS.Tag:MYC} has wing disc | somatic clone phenotype

(Sanders et al., 2009)

Su(H)^del47, ci⁹⁴ has eye disc | somatic clone | cell autonomous phenotype

(Firth and Baker, 2005)

Sos^x122, Su(H)^del47 has eye disc | somatic clone | cell autonomous phenotype

(Firth and Baker, 2005)

Mad^1-2, Su(H)^del47, ci⁹⁴ has eye disc | somatic clone | cell autonomous phenotype

(Firth and Baker, 2005)

Mad^1-2, Su(H)^del47 has eye disc | somatic clone | cell autonomous phenotype

(Firth and Baker, 2005)

Mad¹², Su(H)^del47, ci⁹⁴ has eye disc | somatic clone | cell autonomous phenotype

(Firth and Baker, 2005)

Mad¹², Su(H)^del47 has eye disc | somatic clone | cell autonomous phenotype

(Firth and Baker, 2005)

Mad^1-2, Su(H)^del47, ci⁹⁴ has photoreceptor cell R8 & eye disc | somatic clone phenotype

(Fu and Baker, 2003)

Su(H)^del47, ci⁹⁴ has photoreceptor cell R8 & eye disc | somatic clone phenotype

(Fu and Baker, 2003)

Additional Comments

Genetic Interactions

Statement

Reference

Su(H)^del47/+;CycG^HR7/CycG^HR7 flies display no phenotypic defects in adult wings.

(Nagel et al., 2016)

The suppression of the W^GMR.PG eye phenotype seen in eyes mosaic for Pka-C1^B3 (generated using the ey-FLP/FRT system) is partially reverted if these clones are also mutant for Su(H)^del47.

(Christiansen et al., 2013)

Su(H)^del47 suppresses the presence of ectopic NB-like cells (Dpn and Mira positive) phenotype seen when N^{int.G.Scer\UAS} clones are induced in third instar larval type II NBs under the control of Scer\GAL4^αTub84B.PL.

(San-Juán and Baonza, 2011)

H^E31 Su(H)^del47 double mutant intestinal stem cell (ISC) clones show an overproliferation of small ISC-like cells, as occurs in Su(H)^del47 single mutant clones.

(Bardin et al., 2010)

Expression of arm^{S10.Scer\UAS.T:Hsap\MYC} via Scer\GAL4^αTub84B.PL in Su(H)^del47 clones (using the MARCM technique) increases the size and reduces the number of apoptotic cells compared to Su(H)^del47 clones. arm^{S10.Scer\UAS.T:Hsap\MYC} expression also makes the clones more rounded in appearance but cells do not lose their polarity.

(Sanders et al., 2009)

Su(H)^del47/+ suppresses the wing vein thickening seen in dx¹⁵² hemizygotes.

(Fuwa et al., 2006)

In contrast to Psn^C1 single mutants, the dorsal/ventral boundary forms correctly in Su(H)^del47; Psn^C1 double mutants.

ap^UGO35/ap^rK568; H^E31 and ap^UGO35/ap^rK568; Su(H)^del47/Su(H)⁸ wing discs fail to form a dorsal/ventral compartment boundary.

Unlike expression in clones with a wild-type background, expression of dx^Scer\UAS.cMa (driven by Scer\GAL4^αTub84B.PP) in Su(H)^del47 mutant clones does not result in increased cell proliferation.

(Koelzer and Klein, 2006)

Eye disc cells simultaneously mutant for Mad¹², ci⁹⁴ and Su(H)^del47 continue proliferating instead of arresting in G1 ahead of the morphogenetic furrow. The same phenotype is seen in cells mutant for Mad¹² and ci⁹⁴ - the presence of Su(H)^del47 has no autonomous effect on G1 arrest.

Eye disc cells simultaneously mutant for Mad^1-2, ci⁹⁴ and Su(H)^del47 arrest in G1 after a severe delay but they never enter the second mitotic wave.

Eye disc cells simultaneously mutant for Su(H)^del47 and Mad^1-2, or Su(H)^del47 and ci⁹⁴, do not enter the second mitotic wave.

No second mitotic wave is observed in Su(H)^del47, Sos^x122 clones.

(Firth and Baker, 2005)

R8 photoreceptor differentiation is delayed in ci⁹⁴; Su(H)^del47 double mutant clones, and fails in ci⁹⁴; Mad^1-2; Su(H)^del47 triple mutant clones. This is despite normal R8 differentiation in any of the single mutants (although it is sometimes early in Su(H)^del47 single mutant clones), and in Mad^1-2; Su(H)^del47 double mutants. Ectopic R8 photoreceptor differentiation in ci⁹⁴; Su(H)^del47 somatic clones in eye discs, is much reduced compared to that seen in Su(H)^del47 single mutant clones. This is not the case with Mad^1-2; Su(H)^del47 double mutant clones.

(Fu and Baker, 2003)

Xenogenetic Interactions

Statement

Reference

The wing vein L5 thickening characteristic for adult females expressing Hsap\ATXN1L^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^hh.PU can be suppressed by combination with Su(H)^del47/+, while the loss of posterior crossvein is only partially restored.

(Tong et al., 2011)

Complementation and Rescue Data

Fails to complement

Su(H)^unspecified

(Morel and Schweisguth, 2000)

Rescued by

Su(H)^del47 is rescued by Scer\GAL4^unspecified/Su(H)^UAS.cKa

(Koelzer and Klein, 2003)

Comments

Images (0)

Mutant

Wild-type