FB2024_03 , released June 25, 2024
Allele: Dmel\ap56f
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General Information
Symbol
Dmel\ap56f
Species
D. melanogaster
Name
FlyBase ID
FBal0000643
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Mutagen
Nature of the Allele
Progenitor genotype
Cytology
Description
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DNA sequence
Protein sequence
 
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

ap56f/Df(2R)DG mutant flies show wing phenotypes.

Wing defects are seen in aploxP.MCP-PRE.MM/ap56f mutant flies, but they are significantly less severe than those seen in aploxP.MCP-PRE.MM homozygotes.

Wing defects are seen in Df(2R)DG-Mcp/ap56f mutant flies, but they are less severe than those seen in Df(2R)DG-Mcp homozygotes.

Wing defects are seen in Df(2R)DG/ap56f mutant flies, but they are less severe than those seen in Df(2R)DG homozygotes.

The wing pouch is absent in mutant third larval instar discs.

One day-old ap56f mutant females have a significantly reduced heat stress reactivity as judged by juvenile hormone, octopamine and dopamine metabolism. In contrast, mutant males have a stress reactivity that is similar to wild-type. The initiation of heat shock response appears normal in ap56f females. In normal conditions, ap56f females lay eggs two days later and produce about five times fewer progeny than wild-type females. Egg-counting indicates that the lower rate of fertility in mutants occurs prior to oviposition. Heat stress causes a significant reduction in fertility for only two days in ap56f females, while the fertility of wild-type females is in sharp decline for three days. In normal conditions the viability of both male and female ap56f mutants is reduced (by around 10-15%) compared to wild-type. In wild-type flies survival under heat stress is higher in females, while in ap56f mutants survival is higher in males. Survival of ap56f females under heat stress is sharply decreased compared to wild-type (around 20% survival in 6-day-old ap56f females vs around 50% in wild-type), while survival of mutant males is similar to wild-type.

Mutant females show a delay in vitellogenic development which can be rescued by the application of juvenile hormone III.

Mutant males have smaller accessory glands than normal.

Levels of ecdysteroid production and content in homozygous ovaries are approximately double that of wild-type.

Heterozygotes have normal wings.

Homozygotes exhibit a loss of wing phenotype.

Homozygotes and hemizygotes exhibit nubbin wings. Transheterozygotes with apUG-2106A exhibit wings of nearly normal size with mildly serrated margins.

Ultrastructure of the corpus allatum studied.

Histolysis of the larval fat body is normal in homozygous females.

Homozygous ap56f males have a mating rate 1% of wild type levels, this rate increases with age. If allowed sufficient time with females progeny are produced. For mating success ap56f is an amorph. Homozygous ap56f males showed significantly less nonwing courtship than wild type even though the males were normally active, this is attributable to the ap locus. Homozygous ap56f males had higher sex appeal and orientation component of courtship at 2--3 days old than wild type flies. 2 day old hemizygous ap56f males have a 10--60 fold higher sex appeal than hemizygous ap+ males.

No egg maturation defect. Glands are defective in hormone production.

Homozygous flies have virtually no wing blade and have only 29% female receptivity to mature male ap+ flies. The receptivity is lower for flies heterozygous with Df(2R)M41A4B. Females have a low courtship intensity.

Wings and halteres reduced to vestiges. Scutellar and dorsocentral bristles missing (FBrf0017003). Rear and middle legs occasionally twisted, more frequently in female than in male. Both sexes fertile and long lived when homozygous and in combination with other ap alleles. ap56f/Df(2R)M41A4 have normal complement of dorsocentral and scutellar bristles (FBrf0017003).

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference

ap56f/Df(2R)DG has visible phenotype, suppressible | partially by z1

NOT suppressed by
Statement
Reference

ap56f/Df(2R)DG has visible phenotype, non-suppressible by za

Enhancer of
Statement
Reference

ap56f is an enhancer of visible phenotype of bi1

Other
Statement
Reference
Phenotype Manifest In
NOT Enhanced by
Statement
Reference

ap56f has phenotype, non-enhanceable by Nipped-A222.3

ap56f has phenotype, non-enhanceable by Nipped-A2

ap56f has phenotype, non-enhanceable by Nipped-A323

ap56f has phenotype, non-enhanceable by Nipped-B292.1

ap56f has phenotype, non-enhanceable by Nipped-B407

ap56f has phenotype, non-enhanceable by RpL3845-72

Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference

ap56f/Df(2R)DG has wing phenotype, non-suppressible by za

Enhancer of
Statement
Reference

ap56f is an enhancer of phenotype of Bx3

ap56f is an enhancer of phenotype of Chie5.5

ap56f is an enhancer of wing phenotype of bi1

ap56f/ap[+] is an enhancer of wing phenotype of ctL32, su(Hw)e2

NOT Enhancer of
Statement
Reference

ap56f is a non-enhancer of phenotype of Nnd-1

Other
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

z1 partially suppresses the wing phenotypes seen in ap56f/Df(2R)DG mutant flies.

za does not suppress the wing phenotypes seen in ap56f/Df(2R)DG mutant flies.

Expression of Nintra.-FRT.Scer\UAS under the control of Scer\GAL4αTub84B.PC in ap56f/ap56f wing discs rescues wing growth.

Expression of NECN.Scer\UAS under the control of Scer\GAL4αTub84B.PC in ap56f/ap56f wing discs rescues wing growth.

Clones expressing vgαTub84B.PZa autonomously rescue wing growth in ap56f/ap56f wing discs.

No wing scalloping is seen when combined with SsdpKG03600, SsdpBG01663, Ssdpneo48, Ssdp31 or Ssdp11.

Enhances wing scalloping of Bx3/+.

Chie5.5/ap56f transheterozygotes display wing margin defects: gaps in the posterior margin.

ctL32; su(Hw)e2 flies heterozygous for ap56f display a weak ct wing phenotype.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (11)
Notes on Origin
Discoverer

Thompson, June 1956.

Comments
Comments

Strong ap allele.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (26)