FlyBase Allele Report: Dmel\Nipped-B[407]

Nipped-B⁴⁰⁷/+ heterozygote adults are significantly lighter compared to wild-type, the effect is even more pronounced under limited nutrition conditions (which also leads to developmental delay comparable in length to controls).

Nipped-B⁴⁰⁷/+ females have smaller wings that contain fewer and smaller cells compared to wild-type although the size of cells in third instar larval wing disc (as measured by forward scattering) is not different from controls.

Nipped-B⁴⁰⁷/+ males show more modest reduction in courtship attempts toward an unreceptive female over time compared to wild-type controls, and thusly conditioned heterozygote mutant males do not display reduced courting toward a receptive female as is observed in wild-type, suggesting defects in learning and short-term memory. Nipped-B⁴⁰⁷/+ adult males also display morphological mushroom body defects with high frequency compared to controls.

Nipped-B⁴⁰⁷/+ adults display sex-dependent sleep defects: females sleep less than controls, both during the day and at night, although the total number of both daytime and nighttime sleep episodes is increased. The duration of each sleep episode however is significantly shorter. No significant change in the overall amount of sleep is observed in Nipped-B⁴⁰⁷/+ males although they display increased number of nighttime sleep episodes but these last slightly less long than in controls.

Both Nipped-B⁴⁰⁷/+ adult males and females show defects in circadian activity pattern - all of the females and a significant fraction of males are arrhythmic.

Nipped-B⁴⁰⁷/+ mutant adults do not display any seizure-like behaviors in response to either mechanical or thermal stress.

(Wu et al., 2015)

Heterozygous adults show a significant decrease in wing blade area compared to controls.

(Fasulo et al., 2012)

Chromosome cores and the synaptonemal complex fragment in germarial region 3 or stage 2 oocytes in Nipped-B⁴⁰⁷ heterozygotes, which is substantially earlier than in wild type.

Nipped-B⁴⁰⁷ heterozygotes do not show meiotic chromosome segregation defects.

(Gause et al., 2008)

Approximately 60% of metaphase nuclei in the brains of Nipped-B⁴⁰⁷/Nipped-B⁰²⁰⁴⁷ and Nipped-B⁴⁰⁷ homozygous second instar larvae show precocious sister chromatid separation, in which the centromeric regions of the sister chromatids are somewhat detached from each other.

(Rollins et al., 2004)

Recessive lethality acts at the L2-L3 molt.

(Rollins et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Nipped-B⁴⁰⁷ has visible | female | adult stage phenotype, suppressible by Myc^αTub84B.PBb

(Wu et al., 2015)

Nipped-B⁴⁰⁷ has visible | dominant phenotype, suppressible by Mi-2⁴/Mi-2[+]

(Fasulo et al., 2012)

Enhancer of

Statement

Reference

Nipped-B[+]/Nipped-B⁴⁰⁷ is an enhancer of visible phenotype of Ubx^bx-34e, su(Hw)^e2

(Rollins et al., 1999)

Other

Statement

Reference

Nipped-B[+]/Nipped-B⁴⁰⁷, fs(1)h¹⁸ has partially lethal | dominant phenotype

(Luna-Peláez et al., 2019)

Myc^αTub84B.PBb, Nipped-B⁴⁰⁷ has abnormal cell death | third instar larval stage phenotype

(Wu et al., 2015)

Nipped-B[+]/Nipped-B⁴⁰⁷, Scer\GAL4^Act5C.PI, vtd^RNAi.Sym.UAS has lethal phenotype

(Rollins et al., 2004)

Nipped-B⁴⁰⁷, ct^2s has visible | dominant phenotype

(Rollins et al., 1999)

Phenotype Manifest In

Suppressed by

Statement

Reference

Nipped-B⁴⁰⁷ has wing blade | female phenotype, suppressible by Myc^αTub84B.PBb

(Wu et al., 2015)

Nipped-B⁴⁰⁷ has wing blade phenotype, suppressible by Mi-2⁴/Mi-2[+]

(Fasulo et al., 2012)

Enhancer of

Statement

Reference

Nipped-B[+]/Nipped-B⁴⁰⁷ is an enhancer of abdominal segment | adult stage phenotype of vtd^80Fh-36

(Schaaf et al., 2013)

Nipped-B[+]/Nipped-B⁴⁰⁷ is an enhancer of wing phenotype of ct^L32, su(Hw)^e2

(Rollins et al., 1999)

NOT Enhancer of

Statement

Reference

Nipped-B⁴⁰⁷ is a non-enhancer of phenotype of wg^l-17

(Rollins et al., 1999)

Nipped-B⁴⁰⁷ is a non-enhancer of phenotype of ap^56f

(Rollins et al., 1999)

Nipped-B⁴⁰⁷ is a non-enhancer of phenotype of mam^g2.1

(Rollins et al., 1999)

Nipped-B⁴⁰⁷ is a non-enhancer of phenotype of vg¹

(Rollins et al., 1999)

Nipped-B⁴⁰⁷ is a non-enhancer of phenotype of ct^53d

(Rollins et al., 1999)

Suppressor of

Statement

Reference

Nipped-B⁴⁰⁷ is a suppressor of sex comb | ectopic phenotype of Pc¹

(Schaaf et al., 2013)

Nipped-B⁴⁰⁷ is a suppressor of sex comb | ectopic phenotype of Pc⁶

(Schaaf et al., 2013)

Nipped-B[+]/Nipped-B⁴⁰⁷ is a suppressor of eye phenotype of N^spl-1

(Schaaf et al., 2009)

Nipped-B⁴⁰⁷ is a suppressor of wing phenotype of N^nd-1

(Rollins et al., 1999)

Nipped-B⁴⁰⁷ is a suppressor of eye phenotype of N^spl-1

(Rollins et al., 1999)

NOT Suppressor of

Statement

Reference

Nipped-B⁴⁰⁷ is a non-suppressor of sex comb | ectopic phenotype of Psc¹

(Schaaf et al., 2013)

Nipped-B⁴⁰⁷ is a non-suppressor of sex comb | ectopic phenotype of Sce¹

(Schaaf et al., 2013)

Nipped-B⁴⁰⁷ is a non-suppressor of sex comb | ectopic phenotype of ph-d⁴⁰¹

(Schaaf et al., 2013)

Nipped-B⁴⁰⁷ is a non-suppressor of abdominal segment | adult stage phenotype of ph-d⁴⁰¹

(Schaaf et al., 2013)

Nipped-B⁴⁰⁷ is a non-suppressor of phenotype of N^Ax-E2

(Rollins et al., 1999)

Other

Statement

Reference

Nipped-B⁴⁰⁷, ct^2s has wing phenotype

(Rollins et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

The small wing phenotype characteristic for Nipped-B⁴⁰⁷/+ adults is rescued by expression of Myc^αTub84B.PBb and leads to wings that are even larger than wild-type. Unlike in wild-type, expression of Myc^αTub84B.PBb in the Nipped-B⁴⁰⁷ heterozygous background does not lead to an increase in apoptosis (measured by TUNEL) in third instar larval wing discs.

(Wu et al., 2015)

Mi-2⁴/+ suppresses the reduction in wing blade area seen in Nipped-B⁴⁰⁷/+ flies.

(Fasulo et al., 2012)

Nipped-B⁴⁰⁷/+ shows synthetic lethality with the P{Sym-UAS-Rad21}2 insertion line expressed under the control of Scer\GAL4^Act5C.PI (this particular insertion line does not reduce viability when expressed using Scer\GAL4^Act5C.PI at 25[o]C in an otherwise wild-type background).

(Rollins et al., 2004)

Dominantly enhances the cut wing phenotype of ct^L32, su(Hw)^e2. Dominantly enhances the bithorax phenotype of Ubx^bx-34e, su(Hw)^e2. Shows slight dominant suppression of N^nd-1 wing nick phenotype.

(Rollins et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Nipped-B⁰²⁰⁴⁷

(Rollins et al., 1999)

Partially rescued by

Nipped-B⁴⁰⁷ is partially rescued by Nipped-B^Chi.EGFP

(Gause et al., 2010)

Comments

Nipped-B⁴⁰⁷ homozygotes expressing Nipped-B^{Chi.T:Avic\GFP-EGFP} survive to pupariation at half the expected ratio, with developmental delays.

(Gause et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

407

(Coulthard et al., 2010, Rollins et al., 2004)

Nipped-B⁴⁰⁷

(Grazioli et al., 2021, Luna-Peláez et al., 2019, Pherson et al., 2019, Wu et al., 2015, Schaaf et al., 2013, Fasulo et al., 2012, Coulthard et al., 2010, Gause et al., 2010, Schaaf et al., 2009, Gause et al., 2008)

Name Synonyms

Secondary FlyBase IDs

References (12)

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