FlyBase Allele Report: Dmel\jar[1]

General Information

Symbol

Dmel\jar¹

Species

D. melanogaster

Name

FlyBase ID

FBal0028262

Feature type

allele

Associated gene

Dmel\jar

Associated Insertion(s)

P{PZ}jar¹

Carried in Construct

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Castrillon et al., 1993)

Progenitor genotype

Associated Insertion(s)

P{PZ}jar¹

Cytology

Description

P{PZ} insertion in the first intron.

(Hicks et al., 1999)

Allele components

Component

Use(s)

Inserted element

P{PZ}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased fecundity | male

(Noguchi et al., 2009)

male semi-sterile

male semi-sterile (with Df(3R)crb87-5), with jar^{H-GT.Hsp83.GFP}

(Isaji et al., 2011)

male sterile (with Df(3R)crb87-5)

(Isaji et al., 2011)

male sterile (with Df(3R)crb87-5), with jar^{mWKA.Hsp83.GFP}

(Isaji et al., 2011)

male sterile (with Df(3R)P3-4-7)

(Tomaru et al., 2018)

male sterile (with jar³²²)

(Morrison and Miller, 2008)

male sterile | recessive

(Spradling et al., 1999)

sterile (with Df(3R)crb87-5)

(Noguchi et al., 2009, Noguchi et al., 2006)

viable (with jar³²²)

(Morrison and Miller, 2008)

Phenotype Manifest In

investment cone

(Noguchi et al., 2006)

investment cone (with Df(3R)crb87-5)

(Isaji et al., 2011, Noguchi et al., 2009)

investment cone (with Df(3R)crb87-5), with jar^{Del-N-Gtail.Hsp83.GFP}

(Isaji et al., 2011)

investment cone (with Df(3R)crb87-5), with jar^{HNPMD.Hsp83.GFP}

(Isaji et al., 2011)

investment cone (with Df(3R)crb87-5), with jar^{mLWY.Hsp83.GFP}

(Isaji et al., 2011)

investment cone (with Df(3R)crb87-5), with jar^{mRRL.Hsp83.GFP}

(Isaji et al., 2011)

investment cone (with Df(3R)crb87-5), with jar^{mWKA.Hsp83.GFP}

(Isaji et al., 2011)

seminal vesicle (with Df(3R)crb87-5)

(Noguchi et al., 2009)

spermatid

(Hicks et al., 1999)

spermatid cyst

(Noguchi et al., 2006, Mermall et al., 2005)

spermatid cyst (with Df(3R)crb87-5)

(Noguchi et al., 2009, Noguchi et al., 2006)

spermatozoon

(Hicks et al., 1999)

Detailed Description

Statement

Reference

jar¹/Df(3R)P3-4-7 transheterozygotes are male sterile.

(Tomaru et al., 2018)

When jar^{mLWY.Hsp83.T:Avic\GFP} is expressed in jar¹/Df(3R)crb87-5 mutant animals, the shape of actin cones appear abnormal. Expression of jar^{mLWY.Hsp83.T:Avic\GFP} fails to rescue the fertility defects of jar¹/Df(3R)crb87-5 animals.

When jar^{mWKA.Hsp83.T:Avic\GFP} is expressed in jar¹/Df(3R)crb87-5 mutant animals, the shape of actin cones appear abnormal. Expression of jar^{mWKA.Hsp83.T:Avic\GFP} fails to rescue the fertility defects of jar¹/Df(3R)crb87-5 animals.

Actin cone shapes in jar¹/Df(3R)crb87-5 mutants expressing jar^{mRRL.Hsp83.T:Avic\GFP} appear different from that of wild-type.

Actin cone shapes in jar¹/Df(3R)crb87-5 mutants expressing jar^{Del-N-Gtail.Hsp83.T:Avic\GFP} appear different from that of wild-type.

Actin cone shapes in jar¹/Df(3R)crb87-5 mutants expressing jar^{HNPMD.Hsp83.T:Avic\GFP} appear different from that of wild-type.

(Isaji et al., 2011)

jar¹/Df(3R)crb87-5 spermatid cysts have less actin in the actin cones making them narrower than in wild type. The actin cones travel asynchronously down the axoneme, arresting movement partway along the cyst.

Individualisation does not occur correctly in jar¹/Df(3R)crb87-5 mutants. Some sperm heads are individualised but many are packaged into a single membrane. Many of the sperm heads that have individualised have not undergone cytoplasmic exclusion.

jar¹/Df(3R)crb87-5 seminal vesicles at 1-2 days after eclosion contain very few sperm compared to wild type. A greater number of sperm are present in the seminal vesicles a week after eclosion.

When jar¹\Df(3R)crb87-5 males are mated with wild type females no sperm is found in the female storage organs.

(Noguchi et al., 2009)

Fully elongated jar¹ spermatid cysts prior to individualisation are identical in length and morphology to wild type. Following individualisation, jar¹ cysts appears thin and elongated in comparison with the round cysts seen in wild type.

Unlike in wild type, the 64 spermatids in jar¹ mutants do not undergo individualisation simultaneously. jar¹ cysts are seen that contain both large fused sperm tails and individualised thin tails.

Movement of jar¹ cystic bulges begins at a similar speed to wild type but then slow downs and comes to a stop before reaching the end of the cyst. The distance travelled varies between mutant cysts.

As in wild type, jar¹ mutant sperm tails are surrounded by cytoplasm prior to individualisation. However individualisation does not occur correctly in many jar¹ mutants and several sperm heads are often found within one membrane. The individualised sperm tails that are present in jar¹ mutants fail to undergo the cytoplasmic exclusion that is seen in wild type. The morphology of the axoneme and mitochondrial derivative appears normal in jar¹ mutants.

Before individualisation the quantity of actin in jar¹ cones is similar to wild type. Following the onset of cystic bulge movement the width and length of the actin cones become more variable and uncoordinated in jar¹ mutants compared to wild type and the cones are not aligned in the middle of the cystic bulge. The jar¹ mutant cones fail to accumulate actin during movement and instead the actin density and content are both reduced, resulting in actin cones that are smaller than in wild type with a decreased diameter at the front. However, the basic organisation of the jar¹ cones is normal and no defects in actin filament orientation are seen. Unlike in wild type, cytoplasmic organelles are present in the cone region of jar¹ mutants.

The average speed of cystic bulge movement is reduced in jar¹/Df(3R)crb87-5 mutants compared to wild type.

(Noguchi et al., 2006)

In jar¹ mutants, individualization complexes form within elongated spermatid cysts and begin to migrate, but rapidly fall apart.

(Mermall et al., 2005)

In jar¹ flies, caspase activity can only be detected in spermatid cysts once they are mature enough to contain needle-shaped and condensed nuclei.

(Arama et al., 2003)

Homozygous testes show no obvious differences from wild-type testes. Sperm show defects in motility, but do not show any gross morphological defects. Organisation of the individualisation complex (IC) is abnormal in homozygous testes. Although actin assembles around the nuclei, the individual cones are not aligned properly. Fewer ICs per testis are seen compared to wild type.

(Hicks et al., 1999)

Males are semi-sterile.

(Castrillon et al., 1993)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

jar¹/Df(3R)crb87-5 has male sterile phenotype, suppressible | partially by Sscr\MYO6^Hsp83.GFP

(Isaji et al., 2011)

Phenotype Manifest In

Suppressed by

Statement

Reference

jar¹/Df(3R)crb87-5 has investment cone phenotype, suppressible by Sscr\MYO6^Hsp83.GFP

(Isaji et al., 2011)

Other

Statement

Reference

jar¹, shi¹ has testis phenotype

(Rogat and Miller, 2002)

Additional Comments

Genetic Interactions

Statement

Reference

shi¹/Y jar¹ double mutant males exposed to the non-permissive temperature for shi¹ show a dramatic reduction in the number of individualisation complexes per testis.

(Rogat and Miller, 2002)

Xenogenetic Interactions

Statement

Reference

Expression of Sscr\MYO6^{Hsp83.T:Avic\GFP} rescues cone actin content and shape of jar¹/Df(3R)crb87-5 mutants during spermatogenesis. However, fertility is only partially rescued.

(Isaji et al., 2011)

Complementation and Rescue Data

Fails to complement

jar^mmw14

(Hicks et al., 1999)

Rescued by

jar¹/Df(3R)crb87-5 is rescued by jar^Hsp83.GFP

(Isaji et al., 2011)

jar¹/Df(3R)crb87-5 is rescued by jar^Hsp83.GFP

(Noguchi et al., 2009)

jar¹/Df(3R)crb87-5 is rescued by jar^{Del-Ins1.Hsp83.GFP}

(Noguchi et al., 2009)

jar¹/Df(3R)crb87-5 is rescued by jar^Hsp83.GFP

(Noguchi et al., 2006)

Partially rescued by

jar¹/Df(3R)crb87-5 is partially rescued by jar^{H-GT.Hsp83.GFP}

(Isaji et al., 2011)

jar¹ is partially rescued by jar^UAS.GFP/Scer\GAL4^bab1-Pgal4-2

(Isaji et al., 2011)

jar¹/Df(3R)crb87-5 is partially rescued by jar^{GCN4.Hsp83.GFP}

(Noguchi et al., 2009)

jar¹/Df(3R)crb87-5 is partially rescued by jar^{H-GT.Hsp83.GFP}

(Noguchi et al., 2009)

Not rescued by

jar¹/Df(3R)crb87-5 is not rescued by jar^{Del-N-Gtail.Hsp83.GFP}

(Isaji et al., 2011)

jar¹/Df(3R)crb87-5 is not rescued by jar^{mRRL.Hsp83.GFP}

(Isaji et al., 2011)

jar¹/Df(3R)crb87-5 is not rescued by jar^{mWKA.Hsp83.GFP}

(Isaji et al., 2011)

jar¹/Df(3R)crb87-5 is not rescued by jar^{mLWY.Hsp83.GFP}

(Isaji et al., 2011)

jar¹/Df(3R)crb87-5 is not rescued by jar^{HNPMD.Hsp83.GFP}

(Isaji et al., 2011)

jar¹/Df(3R)crb87-5 is not rescued by jar^{H-GT.Hsp83.GFP}

(Noguchi et al., 2009)

jar¹/Df(3R)crb87-5 is not rescued by jar^{G-tail.Hsp83.GFP}

(Noguchi et al., 2006)

Comments

The actin cone structure of jar¹/Df(3R)crb87-5 mutants is significantly rescued by jar^{H-GT.Hsp83.T:Avic\GFP}. The fertility defects of jar¹/Df(3R)crb87-5 mutants is only partially rescued by jar^{H-GT.Hsp83.T:Avic\GFP}.

Actin cone shapes in jar¹/Df(3R)crb87-5 mutants expressing jar^{mRRL.Hsp83.T:Avic\GFP} appear different from that of wild-type.

Actin cone shapes in jar¹/Df(3R)crb87-5 mutants expressing jar^{Del-N-Gtail.Hsp83.T:Avic\GFP} appear different from that of wild-type.

Actin cone shapes in jar¹/Df(3R)crb87-5 mutants expressing jar^{HNPMD.Hsp83.T:Avic\GFP} appear different from that of wild-type.

Expression of jar^{Hsp83.T:Avic\GFP} rescues the fertility defects of jar¹/Df(3R)crb87-5. The actin cone structure of jar¹/Df(3R)crb87-5 mutants is significantly rescued by jar^{Hsp83.T:Avic\GFP}.

Rescue of the fertility defects of jar¹/Df(3R)crb87-5 by expression of jar^{Scer\UAS.T:Avic\GFP} via Scer\GAL4^bab1-Pgal4-2 is only partial.

(Isaji et al., 2011)

Expression of jar^{Hsp83.T:Avic\GFP} rescues the the actin cone morphology and movement defects seen in jar¹/Df(3R)crb87-5. All sperm tails are correctly individualised and cytoplasm and organelles are removed as in wild type.

Expression of jar^{Hsp83.T:Avic\GFP} rescues the fertility defects seen in jar¹/Df(3R)crb87-5 mutants.

Expression of jar^{Hsp83.T:Avic\GFP} rescues the empty seminal vesicle phenotype seen in jar¹/Df(3R)crb87-5.

Expression of jar^{Hsp83.T:Avic\GFP} rescues the defects in the transfer of sperm to female storage organs that are seen when jar¹/Df(3R)crb87-5 males are mated with wild type females.

Expression of jar^{Hsp83.T:Avic\GFP,T:Scer\GCN4} partially rescues the actin cone morphology and movement defects seen in jar¹/Df(3R)crb87-5.

Expression of jar^{Hsp83.T:Avic\GFP,T:Scer\GCN4} partially rescues the sperm individualisation defects seen in jar¹/Df(3R)crb87-5. Many sperm tails fail to individualise and in those that have, many have not completed cytoplasmic exclusion.

Expression of jar^{Hsp83.T:Avic\GFP,T:Scer\GCN4} partially rescues the fertility defects seen in jar¹/Df(3R)crb87-5 mutants.

Expression of jar^{Hsp83.T:Avic\GFP,T:Scer\GCN4} partially rescues the empty seminal vesicle phenotype seen in jar¹/Df(3R)crb87-5, although many seminal vesicles containing very few sperm are still present.

Expression of jar^{H-GT.Hsp83.T:Avic\GFP} partially rescues actin cone defects seen in jar¹/Df(3R)crb87-5. The cones contain more actin, the shape of the cones is similar to wild type and synchronous cone movement occurs. A more significant rescue is seen when four copies of the transgene are expressed compared to two.

Expression of jar^{H-GT.Hsp83.T:Avic\GFP} partially rescues the sperm individualisation phenotype seen in jar¹/Df(3R)crb87-5. The majority of sperm tails are correctly individualised and cytoplasm and organelles are removed as in wild type.

Expression of jar^{H-GT.Hsp83.T:Avic\GFP} partially rescues the fertility defects seen in jar¹/Df(3R)crb87-5 mutants.

Expression of jar^{H-GT.Hsp83.T:Avic\GFP} partially rescues the empty seminal vesicle phenotype seen in jar¹/Df(3R)crb87-5. Rescue is dose dependent; the phenotype is more significantly improved when four copies of the transgene are expressed rather than two.

Expression of jar^{H-GT.Hsp83.T:Avic\GFP} fails to rescues the defects in the transfer of sperm to female storage organs that are seen when jar¹/Df(3R)crb87-5 males are mated with wild type females.

Expression of jar^{Del-Ins1.Hsp83.T:Avic\GFP} rescues the actin cone morphology and movement defects seen in jar¹/Df(3R)crb87-5 mutants.

Expression of jar^{Del-Ins1.Hsp83.T:Avic\GFP} rescues the fertility defects seen in jar¹/Df(3R)crb87-5.

(Noguchi et al., 2009)

Expression of jar^{Hsp83.T:Avic\GFP} restores the defects in fertility and cystic bulge movement seen in jar¹/Df(3R)crb87-5 mutants.

Expression of jar^{G-tail.Hsp83.T:Avic\GFP} fails to rescue the fertility defects seen in jar¹/Df(3R)crb87-5 mutants.

(Noguchi et al., 2006)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

11793

ry⁵⁰⁶ P{PZ}jar¹/TM3, ry^RK Sb¹ Ser¹

Notes on Origin

Discoverer

A. Spradling.

Comments

Excision of the P{PZ} element indicates that it is responsible for the male sterile phenotype of jar¹.

(Hicks et al., 1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

jar⁰⁶⁷⁴⁶

jar¹

(Tomaru et al., 2018, Hurd et al., 2016, Isaji et al., 2011, Kaplan et al., 2010, Noguchi et al., 2009, Erben et al., 2008, Morrison and Miller, 2008, Frank et al., 2006)

ms(3)06746

(Beaton, 1999.12.12, Spradling et al., 1999)

ms(3)06746⁰⁶⁷⁴⁶

Name Synonyms

Secondary FlyBase IDs

References (19)

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