FlyBase Allele Report: Dmel\jar[mmw14]

General Information

Symbol

Dmel\jar^mmw14

Species

D. melanogaster

Name

FlyBase ID

FBal0102661

Feature type

allele

Associated gene

Dmel\jar

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Hicks et al., 1999)

Progenitor genotype

jar^C865

(Hicks et al., 1999)

Cytology

Description

Imprecise excision of the P{GawB} element resulting in a deletion of 862bp (the whole first exon of jar plus 485bp of sequence flanking the 5' end of the exon and 47bp of sequence flanking the 3' end of the exon is removed).

(Millo et al., 2004)

Imprecise excision of the P{GAL4} element, resulting in a 1kb deletion which removes a portion of the first jar exon.

(Hicks et al., 1999)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female fertile

(Hicks et al., 1999)

male sterile (with jar³²²)

(Morrison and Miller, 2008)

male sterile (with jar^R39)

(Millo and Bownes, 2007, Millo et al., 2004)

male sterile (with jar^R235)

(Millo and Bownes, 2007, Millo et al., 2004)

male sterile | recessive

(Hicks et al., 1999)

viable

(Hicks et al., 1999)

viable (with jar³²²)

(Morrison and Miller, 2008)

Phenotype Manifest In

spermatid

(Hicks et al., 1999)

spermatozoon

(Hicks et al., 1999)

Detailed Description

Statement

Reference

jar^mmw14/Df(3R)crb87-5 hemizygous adults are viable, jar^mmw14/Df(3R)crb87-5 females are fertile and jar^mmw14/Df(3R)crb87-5 males are sterile. Hemizygous testes show no obvious difference in size or morphology compared to wild-type testes. The organisation of the spermatid clusters is not normal; some appear smaller and are closer to the apical end of the testes than normal. Sperm completely lack motility (but do not show any gross morphological defects) and are never observed in the seminal vesicle. Organisation of the individualisation complex (IC) is abnormal in hemizygous testes. The actin filaments of the IC assemble around each nuclear bundle, but often the nuclei are not aligned in register as in wild type. The majority of ICs that colocalise with the nuclear bundles have their component actin cones out of register and therefore have an abnormal morphology. Fewer ICs per testis are seen compared to wild type.

(Hicks et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Complements

jar^R23

(Millo et al., 2004)

jar^R70

(Millo et al., 2004)

Fails to complement

jar^R235

(Millo et al., 2004)

jar^R39

(Millo et al., 2004)

jar^mfw98

(Hicks et al., 1999)

jar¹

(Hicks et al., 1999)

Rescued by

jar^mmw14/jar^R39 is rescued by Scer\GAL4^Pisd-R39/jar^UAS.EGFP

(Millo and Bownes, 2007)

jar^mmw14/jar^R235 is rescued by jar^UAS.EGFP/Scer\GAL4^R235

(Millo and Bownes, 2007)

jar^mmw14/Df(3R)crb87-5 is rescued by jar^Hsp83.PH

(Hicks et al., 1999)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Separable from: a separable lethal mutation; animals homozygous for the jar^mmw14 chromosome die as late third instar larvae, whereas jar^mmw14/Df(3R)crb87-5 hemizygotes are viable.

(Hicks et al., 1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

jar^mmw14

(Morrison and Miller, 2008, Millo and Bownes, 2007)

mmw14

(Hicks et al., 1999)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

Open Close