FlyBase Allele Report: Dmel\jar[H-GT.Hsp83.GFP]

General Information

Symbol

Dmel\jar^{H-GT.Hsp83.GFP}

Species

D. melanogaster

Name

FlyBase ID

FBal0264035

Feature type

allele

Associated gene

Dmel\jar

Associated Insertion(s)

Carried in Construct

P{Hsp83-jar.H-GT.GFP}

Key Links

Transgenic product class

inframe_deletion

(Noguchi et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Noguchi et al., 2009)

Mutagen

in vitro construct

(Noguchi et al., 2009)

Progenitor genotype

Carried in construct

P{Hsp83-jar.H-GT.GFP}

Cytology

Description

A Hsp83 promoter drives expression of a mutated form of full length jar lacking the proximal tail and core coiled-coil domains. The construct is tagged with GFP.

(Noguchi et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp83

Encoded product / tool

jar

Tagged with

GFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

male semi-sterile, with jar¹/Df(3R)crb87-5

(Isaji et al., 2011)

Phenotype Manifest In

Detailed Description

Statement

Reference

The actin cone structure of jar¹/Df(3R)crb87-5 mutants is significantly rescued by jar^{H-GT.Hsp83.T:Avic\GFP}. The fertility defects of jar¹/Df(3R)crb87-5 mutants is only partially rescued by jar^{H-GT.Hsp83.T:Avic\GFP}.

(Isaji et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

jar^{H-GT.Hsp83.GFP} partially rescues jar¹/Df(3R)crb87-5

(Isaji et al., 2011)

jar^{H-GT.Hsp83.GFP} partially rescues jar¹/Df(3R)crb87-5

(Noguchi et al., 2009)

Fails to rescue

jar^{H-GT.Hsp83.GFP} fails to rescue jar¹/Df(3R)crb87-5

(Noguchi et al., 2009)

Comments

(Isaji et al., 2011)

Expression of jar^{H-GT.Hsp83.T:Avic\GFP} partially rescues actin cone defects seen in jar¹/Df(3R)crb87-5. The cones contain more actin, the shape of the cones is similar to wild type and synchronous cone movement occurs. A more significant rescue is seen when four copies of the transgene are expressed compared to two.

Expression of jar^{H-GT.Hsp83.T:Avic\GFP} partially rescues the sperm individualisation phenotype seen in jar¹/Df(3R)crb87-5. The majority of sperm tails are correctly individualised and cytoplasm and organelles are removed as in wild type.

Expression of jar^{H-GT.Hsp83.T:Avic\GFP} partially rescues the fertility defects seen in jar¹/Df(3R)crb87-5 mutants.

Expression of jar^{H-GT.Hsp83.T:Avic\GFP} partially rescues the empty seminal vesicle phenotype seen in jar¹/Df(3R)crb87-5. Rescue is dose dependent; the phenotype is more significantly improved when four copies of the transgene are expressed rather than two.

Expression of jar^{H-GT.Hsp83.T:Avic\GFP} fails to rescues the defects in the transfer of sperm to female storage organs that are seen when jar¹/Df(3R)crb87-5 males are mated with wild type females.

(Noguchi et al., 2009)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

65619

P{Hsp83-jar.H-GT.GFP}1, w^*

65613

w^*; P{Hsp83-jar.H-GT.GFP}2/SM6a

Notes on Origin

Discoverer