FlyBase Allele Report: Dmel\numb[2]

numb² mutant neuroblast clones contain many supernumerary neuroblasts and show an aberrant accumulation of newly-born (ase[+] erm[-]) immature immature Intermediate Neural Progenitors (INPs) compared to controls.

(Janssens et al., 2014)

Homozygous intestinal stem cell clones form large clones over time, as do wild-type controls.

(Bardin et al., 2010)

numb² mutant embryos show normal development of two svp-positive precursors per hemisegment, but these divide to give only two pericardial cells and no svp-positive cardioblasts develop.

In numb² mutant embryos eve-positive pericardial cell numbers double, and eve-positive DA1 muscles are lost.

Both vMP2 and dMP2 siblings adopt the vMP2 fate and extend axons anteriorly in numb² mutant embryos.

(Babaoglan et al., 2009)

In numb² mutants, the pIIb cell is transformed into an ectopic pIIa cell. The most common phenotype of numb² mutants is three socket cells and one shaft cell.

(Koto et al., 2009)

Within numb² pIIa-pIIb cells, an apical actin-rich structure (ARS) is formed normally.

(Rajan et al., 2009)

Single cell mutant clones induced in a single neuroblast generate a clone containing multiple neuroblasts plus neuronal progeny, in contrast to single cell wild-type clones which give rise to a clone containing one neuroblast plus neuronal progeny.

(Lee et al., 2006)

100% of heterozygous flies show rhythmic locomotor activity, with a period of 24.2 +/- 0.1 hours. 94% of numb²/numb^SW flies show rhythmic locomotor activity, with a period of 24.3 +/- 0.1 hours.

(Stempfl et al., 2002)

Large homozygous clones in the head result in a multiple socket phenotype in the sensory organs.

(Ben-Yaacov et al., 2001)

Glial development in the NGB 6-4T lineage is not affected in mutant embryos.

(Freeman and Doe, 2001)

6.0 odd-expressing pericardial cells and 4.2 cardioblasts develop per abdominal hemisegment in mutant embryos (in contrast to the wild-type number of 4.2 odd-expressing pericardial cells and 6.0 cardioblasts per abdominal hemisegment). The precise alignment of the cardioblasts is disrupted leading to "broken rows" of cardioblasts in mutant embryos.

(Ward and Skeath, 2000)

Both progeny from the GMC4-2a ganglion mother cell appear to adopt the RP2sib neuronal fate in about 1/4 cases. Embryos lacking both maternal and zygotic numb, produced by making numb⁴ germline clones, display near-complete expressivity of numb phenotypes. Both progeny from the GMC4-2a ganglion mother cell appear to adopt the RP2sib neuronal fate. Both progeny from the GMC1-1a ganglion mother cell appear to adopt the pCC neuronal fate. Both progeny from the ganglion mother cell that normally mothers the U/CQ and U/CQ sibling neurons appear to adopt the U/CQ neuron fate. EL neurons almost completely fail to appear. Maternal loss alone has almost no effect on RP2/RP2sib, U/Usib, EL, and pCC/aCC lineages. Zygotic loss of numb² allows about half the wild-type number of RP2 neurons, about 7 times the wild-type number of U/CQ neurons, and about 6% of the normal number of EL neurons to develop. the pCC/aCC lineage appears to be unaffected.

(Lear et al., 1999)

numb² embryos lack the DA1 muscles and have twice the normal number of eve-expressing pericardial cells (EPCs). numb²; zfh1² double mutant embryos have no EPCs and no DA1 muscles, except for rarely 1 or 2 EPCs are present.

(Su et al., 1999)

Many muscles are absent in numb¹/numb² embryos, particularly in the dorsal region. Most myocardial cells are formed. The number of eve-expressing pericardial cells is double that of wild-type.

(Park et al., 1998)

The RP2sib neuron, vMP2 neuron and U neuron are all duplicated at the expense of their siblings. The number of EL neurons is strongly reduced. pCC/aCC are unaffected. This phenotype is the reciprocal of that shown for spdo, Dl, N and mam embryos. The phenotype of numb; spdo double mutants is identical to embryos lacking spdo alone. The eve⁺ EL neurons are completely rescued.

(Skeath and Doe, 1998)

Homozygous clones in the IIa cell due to Scer\FLP1^Scer\UAS.cBa expression driven by Scer\GAL4^sca-109-68 cause a double-socket phenotype. Clones generated from Scer\GAL4^sca-537.4 cause sense organs with three sockets and one hair or four sockets.

(Wang et al., 1997)

Homozygous clones induced in the sensory organ precursor daughter IIa cells of the adult external sense organs, when Scer\FLP1^Scer\UAS.cBa expression is driven by Scer\GAL4^sca-109-68, produce a double socket phenotype.

(Frise et al., 1996)

apparently null since the neuronal phenotype of homozygotes is indistinguishable from that of hemizygotes.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

numb² has visible phenotype, suppressible by Su(H)⁸

(Wang et al., 1997)

Suppressor of

Statement

Reference

numb[+]/numb² is a suppressor of abnormal planar polarity | male | adult stage phenotype of dsh¹

(Domingos et al., 2019)

Df(1)N-81k1, numb[+], numb², + is a suppressor of abnormal size phenotype of Scer\GAL4^ptc-559.1, spdo^UAS.cOa

(Babaoglan et al., 2009)

Phenotype Manifest In

Enhanced by

Statement

Reference

numb² has embryonic heart cardioblast | embryonic stage 16 phenotype, enhanceable by Scer\GAL4^twi.PG/spdo^UAS.cOa

(Babaoglan et al., 2009)

Suppressed by

Statement

Reference

numb² has embryonic pericardial cell | ectopic | embryonic stage 16 phenotype, suppressible by Scer\GAL4^twi.PG/spdo^UAS.cOa

(Babaoglan et al., 2009)

numb² has trichogen cell phenotype, suppressible by Su(H)⁸

(Wang et al., 1997)

Enhancer of

Statement

Reference

numb[+]/numb² is an enhancer of eo neuron phenotype of l(2)gl^ts3

(Ohshiro et al., 2000)

numb[+]/numb² is an enhancer of tormogen cell phenotype of l(2)gl^ts3

(Ohshiro et al., 2000)

numb[+]/numb² is an enhancer of external sensory organ phenotype of l(2)gl^ts3

(Ohshiro et al., 2000)

NOT Enhancer of

Statement

Reference

numb² is a non-enhancer of embryonic pericardial cell phenotype of N^UAS.cBa, Scer\GAL4^twi.PG, spdo^G104

(Babaoglan et al., 2009)

numb² is a non-enhancer of embryonic pericardial cell phenotype of Scer\GAL4^twi.PG, fng^UAS.cKa, spdo^G104

(Babaoglan et al., 2009)

Suppressor of

Statement

Reference

numb[+]/numb² is a suppressor of ommatidium | male phenotype of dsh¹

(Domingos et al., 2019)

numb[+]/numb² is a suppressor | partially of scutellar bristle | ectopic phenotype of Scer\GAL4^ptc-559.1, spdo^UAS.cOa

(Babaoglan et al., 2009)

Df(1)N-81k1, numb[+], numb², + is a suppressor | partially of wing phenotype of Scer\GAL4^nub-AC-62, spdo^UAS.cOa

(Babaoglan et al., 2009)

N^UAS.cBa, Scer\GAL4^twi.PG, numb² is a suppressor of embryonic pericardial cell phenotype of spdo^G104

(Babaoglan et al., 2009)

Scer\GAL4^twi.PG, numb², fng^UAS.cKa is a suppressor of embryonic pericardial cell phenotype of spdo^G104

(Babaoglan et al., 2009)

Df(1)N-81k1, numb[+], numb², + is a suppressor | partially of wing vein phenotype of Scer\GAL4^nub-AC-62, spdo^UAS.cOa

(Babaoglan et al., 2009)

Df(1)N-81k1, numb[+], numb², + is a suppressor | partially of wing margin phenotype of Scer\GAL4^nub-AC-62, spdo^UAS.cOa

(Babaoglan et al., 2009)

numb[+]/numb² is a suppressor of embryonic heart cardioblast | increased number | embryonic stage 16 phenotype of Scer\GAL4^twi.PG, spdo^UAS.cOa

(Babaoglan et al., 2009)

numb[+]/numb² is a suppressor of embryonic pericardial cell | increased number | embryonic stage 16 phenotype of Scer\GAL4^twi.PG, spdo^UAS.cOa

(Babaoglan et al., 2009)

numb² is a suppressor of embryonic heart cardioblast | increased number | embryonic stage 16 phenotype of Scer\GAL4^twi.PG, spdo^UAS.cOa

(Babaoglan et al., 2009)

numb² is a suppressor of embryonic pericardial cell | increased number | embryonic stage 16 phenotype of Scer\GAL4^twi.PG, spdo^UAS.cOa

(Babaoglan et al., 2009)

Other

Statement

Reference

Klp98A[+]/Klp98A^Δ47, numb^SW/numb² has tormogen cell | adult stage phenotype

(Derivery et al., 2015)

Klp98A^Δ6/Klp98A^Δ47, numb^SW/numb² has tormogen cell | adult stage phenotype

(Derivery et al., 2015)

Klp98A^Δ8/Klp98A^Δ47, numb^SW/numb² has tormogen cell | adult stage phenotype

(Derivery et al., 2015)

Rca1^33X16, numb² has ganglion mother cell GMC4-2a phenotype

(Lear et al., 1999)

Rca1^33X16, numb² has ganglion mother cell GMC1-1a phenotype

(Lear et al., 1999)

Rca1^33X16, numb² has ganglion mother cell phenotype

(Lear et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

Klp98A^Δ47/+;numb²/numb^SW or Klp98A^Δ47/+;numb^SW/numb¹⁵ mutants have a multiple socket phenotype on the notum; this phenotype is suppressed in Klp98A^Δ47/Klp98A^Δ6;numb²/numb^SW, Klp98A^Δ47/Klp98A^Δ8;numb²/numb^SW or Klp98A^Δ47/Klp98A^Δ6;numb¹⁵/numb^SW double mutants.

(Derivery et al., 2015)

Expression of spdo^Scer\UAS.cOa under the control of Scer\GAL4^twi.PG in a numb² mutant embryo blocks the formation of svp-positive precursor cells, resulting in dramatically fewer svp-positive heart cells and less cardioblasts. Fewer svp-negative cardioblasts develop.

eve-positive equivalence groups form but less eve-positive pericardial cells are seen when spdo^Scer\UAS.cOa is expressed under the control of Scer\GAL4^twi.PG in a numb² background.

Expression of N^Scer\UAS.cBa under the control of Scer\GAL4^twi.PG in a numb² and spdo^G104 mutant background increases the number of eve-positive pericardial cells, and decreases the number of eve-positive DA1 muscle numbers compared to embryos mutant for spdo^G104. However, there is no change observed in this increased number of eve-positive pericardial cells between spdo^G104 embryos also expressing N^Scer\UAS.cBa under the control of Scer\GAL4^twi.PG alone, or in combination with numb².

Expression of fng^Scer\UAS.cKa under the control of Scer\GAL4^twi.PG in a numb² and spdo^G104 mutant background increases the number of eve-positive pericardial cells, and decreases the number of eve-positive DA1 muscle numbers compared to embryos mutant for spdo^G104. However, there is no change observed in this increased number of eve-positive pericardial cells between spdo^G104 embryos also expressing fng^Scer\UAS.cKa under the control of Scer\GAL4^twi.PG alone, or in combination with numb².

Simultaneously expressing spdo^Scer\UAS.cOa under the control of Scer\GAL4^nub-AC-62 in a Df(1)N-81k1/+ and numb²/+ background partially suppresses wing notching, vein thickening, and reduced wing size observed in the absence of numb².

There is a slightly reduced number of ectopic scutellar bristles in flies expressing spdo^Scer\UAS.cOa under the control of Scer\GAL4^nub-AC-62 in a numb²/+ background.

Expression of spdo^Scer\UAS.cOa under the control of Scer\GAL4^twi.PG in the presence of numb²/+ suppresses the formation of extra svp-positive heart precursors by stage 12, and extra progenitor svp-positive pericardial cells, to almost wildtype levels.

(Babaoglan et al., 2009)

Clones induced

(Lee et al., 2006)

Large numb² clones in the head of WASp¹/Df(3R)3450 animals rarely show the numb² multiple socket phenotype, while the WASp¹/Df(3R)3450 smooth cuticle phenotype predominates, indicating that WASp is epistatic to numb.

(Ben-Yaacov et al., 2001)

In Rca1^33X16, numb² double mutants the GMC4-2a ganglion mother cell fails to divide and adopts the RP2sib neuron fate in about 1 in 2 cases. In Rca1^33X16, numb² double mutants the GMC1-1a ganglion mother cell fails to divide and adopts the pCC neuron fate in about 3/4 of cases. In Rca1^33X16, numb² double mutants the ganglion mother cell that normally mothers the U/CQ and U/CQ sibling neurons fails to divide and often adopts the U/CQ sibling neuron fate.

(Lear et al., 1999)

Su(H)⁸ mutation dominantly suppresses the double socket phenotype, but not the double sheath phenotype, of numb^SW/numb² mutants. Double mutant clones (numb² Su(H)⁸) exhibit the phenotype caused due to loss of function of Su(H) alone: twinned hair phenotype.

(Wang et al., 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

numb^nuts

(Stempfl et al., 2002)

Rescued by

numb²/numb¹⁵ is rescued by numb^{+tCH322-113H20}

(Couturier et al., 2013)

numb²/numb¹⁵ is rescued by numb^GFP

(Couturier et al., 2013)

Partially rescued by

numb² is partially rescued by Scer\GAL4^{neur-GAL4-A101}/numb^PTB.UAS.GFP

(Justice et al., 2003)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

On the basis of CNS phenotype numb alleles form a series: numb² > numb⁴ > numb¹/numb³.

(Skeath and Doe, 1998)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

nb²

(Tong et al., 2010, Roegiers et al., 2005, Lear et al., 1999, Skeath and Doe, 1998)

numb2

(Jagla et al., 2002)

numb²

(Wu et al., 2023, Domingos et al., 2019, Schwarz et al., 2018, Derivery et al., 2015, Janssens et al., 2014, Couturier et al., 2013, Xiao et al., 2012, Rebeiz et al., 2011, Bardin et al., 2010, Monastirioti et al., 2010, Babaoglan et al., 2009, Koto et al., 2009, Lacin et al., 2009, Rajan et al., 2009, Lee et al., 2006, Emery et al., 2005)

Name Synonyms

Secondary FlyBase IDs

References (35)

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