FlyBase Allele Report: Dmel\Nrg[l10]

Nrg^l10/Nrg⁸⁴⁹ and Nrg^l10/Nrg⁸⁹² females show normal sexual receptivity at 18 and 25[o]C, but show reduced receptivity at 29[o]C when mated to wild-type males.

(Carhan et al., 2005)

Homozygous embryos raised at the restrictive temperature show altered motoneuron pathfinding trajectories.

(Bouley et al., 2000)

When Nrg^l10 embryos and larvae are raised at the permissive temperature (17^oC) and shifted to the restrictive temperature (29^oC) during early to middle third instar larvae, abnormalities are seen in ocellar sensory system (OSS) axon pathfinding without alterations in cell number or position. Ocellar pioneer (OP) axons become attached to the epidermis, where they can fasciculate with bristle mechanosensory (BM) axons. Secondly OP axons fail to cross the brain at the appropriate choice point. After head eversion, this alteration is revealed as an OP nerve projecting inside the head. free and away from the epidermis but heading and terminating at an ectopic position. Thirdly BM axons take abnormal trajectories. Although they initially project in the correct direction, sometimes when reaching the region where they should leave the epidermal surface and project to the brain, they turn backward and project away from their normal trajectory. Forth BM axons sometimes stall in the epidermis. Fifth, some BM axons, affter head eversion, project separated from the epidermis.

(Garcia-Alonso et al., 2000)

Homozygotes are viable at 18^oC and 21^oC. At 25^oC, viability of homozygous embryos is 22% and those embryos that do hatch die as first instar larvae shortly after hatching. No homozygous embryos survive to hatching at 29^oC. The temperature-sensitive period for lethality is between midstage 15 and late stage 16 of embryonic development. Homozygous embryos show a general disorganisation of the peripheral nervous system cell bodies when raised at the restrictive temperature of 29^oC. The lateral pentascolopidial chordotonal organ (lch5) cells are rounded and irregularly positioned. The apical dendrites and basal anchoring process are disorganised. The disorganisation of the lch5 appears to displace most of the other cells of the lateral cluster, including the lateral campaniform sensilla, the lateral trichoid sensilla and the lateral monoscolopidial chordotonal organ. Fasciculation of the peripheral sensory axon tracts appears relatively normal. The aCC and SNb motoneurons often show a stalled phenotype, failing to extend normally, and SNb motoneurons sometimes show abnormal contacts with their targets.

(Hall and Bieber, 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Nrg^l10 is an enhancer of abnormal neuroanatomy phenotype of unc-5⁸

(Zarin et al., 2014)

beat-Ia^C163, Nrg^l10, beat-Ia³ is an enhancer of abnormal neuroanatomy phenotype of unc-5⁸

(Zarin et al., 2014)

NOT Enhancer of

Statement

Reference

Nrg^l10 is a non-enhancer of abnormal neuroanatomy phenotype of beat-Ia^C163/beat-Ia³, unc-5⁸

(Zarin et al., 2014)

NOT Suppressor of

Statement

Reference

Nrg^l10 is a non-suppressor of abnormal neuroanatomy phenotype of beat-Ia^C163/beat-Ia³, unc-5⁸

(Zarin et al., 2014)

Phenotype Manifest In

Enhanced by

Statement

Reference

Nrg^l10 has axon & mechanosensory neuron & adult head | conditional ts phenotype, enhanceable by Scer\GAL4^MS1075/htl^λ.UAS

(Garcia-Alonso et al., 2000)

Nrg^l10 has axon & ocellus sensory structure | conditional ts phenotype, enhanceable by Egfr^unspecified

(Garcia-Alonso et al., 2000)

Nrg^l10 has axon & mechanosensory neuron & adult head | conditional ts phenotype, enhanceable by Egfr^unspecified

(Garcia-Alonso et al., 2000)

Suppressed by

Statement

Reference

Nrg^l10 has axon & adult head | conditional ts phenotype, suppressible by Scer\GAL4^MS1075/htl^λ.UAS

(Garcia-Alonso et al., 2000)

Nrg^l10 has axon & adult head | conditional ts phenotype, suppressible by htl^UAS.cMa/Scer\GAL4^MS1075

(Garcia-Alonso et al., 2000)

Nrg^l10 has axon & ocellus sensory structure | conditional ts phenotype, suppressible by Egfr^E3

(Garcia-Alonso et al., 2000)

Nrg^l10 has axon & mechanosensory neuron & adult head | conditional ts phenotype, suppressible by Egfr^E3

(Garcia-Alonso et al., 2000)

Enhancer of

Statement

Reference

Nrg^l10 is an enhancer of motor neuron phenotype of unc-5⁸

(Zarin et al., 2014)

Nrg^l10 is an enhancer of larval intersegmental nerve phenotype of unc-5⁸

(Zarin et al., 2014)

beat-Ia^C163, Nrg^l10, beat-Ia³ is an enhancer of larval intersegmental nerve phenotype of unc-5⁸

(Zarin et al., 2014)

NOT Enhancer of

Statement

Reference

Nrg^l10 is a non-enhancer of motor neuron phenotype of beat-Ia^C163/beat-Ia³, unc-5⁸

(Zarin et al., 2014)

NOT Suppressor of

Statement

Reference

Nrg^l10 is a non-suppressor of motor neuron phenotype of beat-Ia^C163/beat-Ia³, unc-5⁸

(Zarin et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Nrg^l10; unc-5⁸ double mutants exhibit aberrant motoneuron crossing to the adjacent segment before the first branch point. The number of late defects is also substantially increased compared to both single mutants.

Nrg^l10/Y; beat-Ia^C163/beat-Ia³ double mutants exhibit intersegmental nerve axon stalling prior to the first branch point.

Nrg^l10/Y; unc-5⁸, beat-Ia^C163/beat-Ia³ triple mutants exhibit severe defects in the intersegmental nerve crossing in the ventral muscle field.

Nrg^l10/Y; unc-5⁸/unc-5⁸ double mutants do not exhibit any aCC or RP2 exit phenotypes.

Nrg^l10/Y; beat-Ia^C163/beat-Ia³ double mutants do not exhibit any aCC or RP2 exit phenotypes.

The addition of a Nrg^l10/Y background does not affect the unc-5⁸, beat-Ia^C163/beat-Ia³ CNS exit failure seen in 10% of unc-5⁸, beat-Ia^C163/beat-Ia³ hemisegments.

Removing one copy of eve in Nrg^l10/Y mutants results in more severe intersegmental nerve guidance defects with a significant increase in axon stalling (from 3% to 21.2% upon the addition of eve^ΔRP2A).

(Zarin et al., 2014)

The addition of htl^{Scer\UAS.T:λ\cI-DD} (when driven by Scer\GAL4^MS1075) to Nrg^l10 animals reduces the expressivity and penetrance of axon guidance defects. The frequency of axon guidance alterations are halved, though bristle mechanosensory (BM) axon extension defects are significantly enhanced. The addition of htl^Scer\UAS.cMa (when driven by Scer\GAL4^MS1075) to Nrg^l10 animals reduces the expressivity of axon guidance defects. The addition of htl^AB42 to Nrg^l10 animals shows a very mild enhancement of the axon guidance defects. The addition of Egfr^unspecified to Nrg^l10 animals shows an enhancement of the axon guidance defects. The addition of Egfr^E3 or Egfr^E1 to Nrg^l10 animals shows a suppression of the axon guidance defects.

(Garcia-Alonso et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Complements

Nrg^ibx

(Carhan et al., 2005)

Fails to complement

Nrg^ibx

(Carhan et al., 2005)

Nrg^l7

(Carhan et al., 2005)

Nrg^l4

(Carhan et al., 2005)

Nrg^l7

(Hall and Bieber, 1997)

Nrg^l4

(Hall and Bieber, 1997)

Partially rescued by

Nrg^l10 is partially rescued by Scer\GAL4^MS1075/Nrg^180.UAS

(Garcia-Alonso et al., 2000)

Comments

Nrg^l10 complements the reduced female receptivity phenotype of Nrg^ibx at 18 and 25[o]C, but fails to complement this phenotype at 29[o]C. Nrg^ibx complements the lethality of Nrg^l10 seen at 25[o]C.

Nrg^l10 complements the reduced female receptivity phenotype of Nrg⁸⁹² at 18 and 25[o]C, but fails to complement this phenotype at 29[o]C.

(Carhan et al., 2005)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

24167

C(1)A, y¹/y¹ w^* Nrg^l10

Notes on Origin

Discoverer

Digan.

(King et al., 1986)

Comments

Complements: ibx^M72.

(Kerr et al., 1997)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

Nrg¹⁰

(Kerr et al., 1997)

Nrg³

(Goossens et al., 2011)

Nrg^l10

l(1)7Fa¹⁰

l(1)B4

(Kerr et al., 1997, King et al., 1986)

nrg³

(Clements et al., 2021, Zarin et al., 2014, Carhan et al., 2005, Kang et al., 2001, Bouley et al., 2000, Garcia-Alonso et al., 2000, Hall and Bieber, 1997)

Name Synonyms

Secondary FlyBase IDs

References (10)

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