FlyBase Allele Report: Dmel\Nrg[ibx]

General Information

Symbol

Dmel\Nrg^ibx

Species

D. melanogaster

Name

icebox

FlyBase ID

FBal0004430

Feature type

allele

Associated gene

Dmel\Nrg

Associated Insertion(s)

Carried in Construct

Also Known As

ibx

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

Progenitor genotype

Cytology

Description

Amino acid replacement: G92R.

(Carhan et al., 2005)

Nucleotide substitution: G?A.

(Carhan et al., 2005)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:8,544,920..8,544,920 [+]

Nucleotide change:

G8544920A

Reported nucleotide change:

G?A

Amino acid change:

Reported amino acid change:

G92R

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Carhan et al., 2005)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal courtship behavior | female (with Df(1)KA14)

(Carhan et al., 2005)

abnormal courtship behavior | female (with Df(1)RA2)

(Carhan et al., 2005)

abnormal courtship behavior | female (with Nrg^G0099)

(Carhan et al., 2005)

abnormal courtship behavior | female (with Nrg^G0413)

(Carhan et al., 2005)

abnormal courtship behavior | female (with Nrg^l4)

(Carhan et al., 2005)

abnormal courtship behavior | female | recessive

(Carhan et al., 2005, Kerr et al., 1997)

abnormal courtship behavior | male

(Carhan et al., 2005)

abnormal mating | female | recessive

(Kerr et al., 1997)

abnormal neuroanatomy | adult stage

(Carhan et al., 2005)

fertile

(Kerr et al., 1997)

lethal | maternal effect | recessive

viable

(Kerr et al., 1997)

Phenotype Manifest In

adult antennal lobe

(Carhan et al., 2005)

adult central complex

(Carhan et al., 2005)

adult mushroom body

(Carhan et al., 2005)

ellipsoid body

(Carhan et al., 2005)

lobe system of adult mushroom body

(Carhan et al., 2005)

seminal receptacle | maternal effect

Detailed Description

Statement

Reference

The mating frequency of homozygous females mated to wild-type males is markedly reduced (23%) relative to wild-type (97%) and heterozygous (86%) controls.

Homozygous females have defects in the mushroom bodies, central complex (especially the ellipsoid body) and antennal lobe. The mushroom body lobes are either reduced in size or completely missing and an abnormal whorl of neuronal processes is seen just ventral to the calyces. The ellipsoid body fails to fuse into a single structure (as occurs in wild type). These defects are seen in both males and females.

Homozygous males show defects in courtship behaviour relative to wild-type controls when mated to wild-type females. The mean latency is not significantly different from wild type, but they show significantly extended mating speeds. Once they are courting a female, the mutant males tend to have more breaks in their courtship than normal.

(Carhan et al., 2005)

Homozygous and hemizygous mutant flies are fertile except for a few never-mating females. The mating frequency of homozygous females is reduced about 4-fold compared to wild-type levels, for virgins aged between 1 to 12 days. The largest difference from wild type occurs at 3 days of age, when the mating frequency of homozygous females averages 21% versus 88% of wild-type controls. The changes in receptivity with age are similar in homozygous and wild-type females. The mutation does not appear to be temperature sensitive (in terms of temperature of development). The sex appeal of homozygous females (measured as minutes of wing vibration directed towards the female by a male in a 5 minute test) is indistinguishable from that of wild-type females. Homozygous females show rejection behaviour (such as decamping, wing flicking, kicking, fending and ovipositor extrusion) in response to male courtship more frequently than control females. General locomotor activity of homozygous females, during courtship with wild-type males, is not different from wild type. Mutant males do not differ significantly in any component of courtship, except that mutant males give significantly more bouts of abdomen bending than wild-type males. The mating success of mutant males is not significantly different from that of wild-type males. The egg-to-adult viability of homozygotes and the progeny of a cross between homozygotes and wild type is normal. Ovarian development of homozygous females is normal. Attraction to ethanol and avoidance of 3-octanol is normal in mutant males and females, and photobehaviour is normal. The heart rate and rhythmicity of heartbeat of homozygous pupae is not significantly different wild type. Treatment with methoprene results in more mating in homozygous females.

(Kerr et al., 1997)

No sperm is stored in hemizygous females.

(Mohler and Carroll, 1984)

Lays eggs. No sperm stored in hemizygous female.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

Nrg^ibx is partially rescued by Nrg^167.UAS/Scer\GAL4^c316

(Carhan et al., 2005)

Nrg^ibx is partially rescued by Nrg^167.UAS/Scer\GAL4^Mef2.247

(Carhan et al., 2005)

Nrg^ibx is partially rescued by Nrg^180.I.UAS/Scer\GAL4^c316

(Carhan et al., 2005)

Nrg^ibx is partially rescued by Nrg^180.I.UAS/Scer\GAL4^Mef2.247

(Carhan et al., 2005)

Nrg^ibx is partially rescued by Nrg^180.I.UAS/Scer\GAL4^30Y

(Carhan et al., 2005)

Not rescued by

Nrg^ibx is not rescued by Nrg^167.UAS/Scer\GAL4^30Y

(Carhan et al., 2005)

Nrg^ibx is not rescued by Nrg^167.UAS/Scer\GAL4^Tab2-201Y

(Carhan et al., 2005)

Nrg^ibx is not rescued by Nrg^180.I.UAS/Scer\GAL4^Tab2-201Y

(Carhan et al., 2005)

Comments

The reduced female receptivity phenotype of Nrg^ibx is complemented by Nrg⁸⁴⁹, Nrg^l7, Nrg^BG01674, Nrg^G00305 and Nrg⁸⁴⁹.

Nrg^ibx and Nrg⁸⁹² complement each other with respect to the reduced female receptivity phenotype seen in each homozygote.

Nrg^l4, Df(1)KA14, Df(1)RA2, Nrg^G0099, Nrg^G0413 and Nrg^G0488b each fail to complement the reduced female receptivity phenotype of Nrg^ibx.

Nrg^l10 complements the reduced female receptivity phenotype of Nrg^ibx at 18 and 25[o]C, but fails to complement this phenotype at 29[o]C. Nrg^ibx complements the lethality of Nrg^l10 seen at 25[o]C.

The reduced female receptivity phenotype of Nrg^ibx is complemented by gg², lawc^EF520, otu⁷, ptg⁷, Df(1)Desi-S3, Df(1)Sxl-bt, fs(1)M112¹, In(1)S, In(1)sc⁷, l(1)7Ed¹, l(1)ESHS38¹, IntS4^G0095, l(1)G0178^G0178, sdt^XN, P{GT1}BG02451 and P{NM}8A.

Expression of Nrg^167.Scer\UAS under the control of Scer\GAL4^c316 results in substantial rescue of the Nrg^ibx central brain phenotype.

Expression of Nrg^167.Scer\UAS under the control of either Scer\GAL4^Mef2.247, Scer\GAL4^30Y or Scer\GAL4^Tab2-201Y does not appear to rescue the Nrg^ibx central brain phenotype.

Expression of Nrg^167.Scer\UAS under the control of either Scer\GAL4^Mef2.247 or Scer\GAL4^c316 results in a significant increase in the sexual receptivity of Nrg^ibx females, while expression under the control of Scer\GAL4^Tab2-201Y has no significant effect.

Expression of Nrg^167.Scer\UAS under the control of Scer\GAL4^30Y results in a small but significant decrease in the sexual receptivity of Nrg^ibx females.

Expression of Nrg^{180.I.Scer\UAS} under the control of either Scer\GAL4^Mef2.247, Scer\GAL4^30Y, Scer\GAL4^Tab2-201Y or Scer\GAL4^c316 does not result in any clear evidence of rescue the Nrg^ibx central brain phenotype.

Expression of Nrg^{180.I.Scer\UAS} under the control of either Scer\GAL4^Mef2.247, Scer\GAL4^30Y or Scer\GAL4^c316 results in a small but significant increase in the sexual receptivity of Nrg^ibx females, while expression under the control of Scer\GAL4^Tab2-201Y has no significant effect.

(Carhan et al., 2005)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Complements: otu⁵. Complements: otu⁷. Complements: Nrg^l7. Complements: Nrg^l10. Complements: Nrg^unspecified. Complements: pt³. Complements: l(1)7Fc¹. Complements: l(1)7Fd². Complements: gg². Complements: oc⁹. Complements: l(1)8Aa⁸. Complements: l(1)8Ad¹.

(Kerr et al., 1997)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

14-677

(Mohler and Carroll, 1984)

Nrg^ibx

fs(1)M72

(Kerr et al., 1997)

fs(1)M72¹

ibx

(Carhan et al., 2005, Kerr et al., 1997)

ibx^M72

Name Synonyms

icebox

(Carhan et al., 2005)

Secondary FlyBase IDs

References (3)

Report Sections

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