FB2024_03 , released June 25, 2024
Allele: Dmel\Nrg180.I.UAS
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General Information
Symbol
Dmel\Nrg180.I.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0147728
Feature type
allele
Associated gene
Dmel\Nrg
Associated Insertion(s)
Carried in Construct
P{UAS-nrg180}
Also Known As
UAS-nrg180, UAS-nrg, UAS-nrg180, UAS-NRG-180
Key Links
Transgenic product class
wild_type
(Islam et al., 2003)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Islam et al., 2003)
Mutagen
in vitro construct
(Islam et al., 2003)
Progenitor genotype
Carried in construct
P{UAS-nrg180}
Cytology
Description

The MtnA promoter is replaced with UASt sequences.

(Islam et al., 2003)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Nrg
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      ameliorates  epilepsy
      modeled by pkIG1-1
      (Chen et al., 2023)
      modeled by pk325
      (Chen et al., 2023)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Embryos with Nrg180.I.Scer\UAS driven by Scer\GAL4elav.PU do not have intersegmental nerve branch ISNb motor axon innervation defects; when driven by Scer\GAL4how-24B, there is an ISNb axon arrest phenotype.

      (Lu et al., 2014)

      Expression of Nrg180.I.Scer\UAS in a Df(2R)eve mutant background, under the control of Scer\GAL4eve.RN2 leads to both aCC and RP2 motor neurons displaying an almost exclusively single exit from the CNS.

      (Zarin et al., 2014)

      The number of dendritic endpoints of dorsal dendritic arborisation neurons is reduced compared to controls in larvae expressing Nrg180.I.Scer\UAS under the control of Scer\GAL4elav-C155.

      (Yang et al., 2011)

      When Nrg180.I.Scer\UAS is driven by Scer\GAL4GMR.PF no effects are seen on the number of photoreceptor cells or cone cells present in the eye.

      (Islam et al., 2003)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Enhancer of
      Suppressor of
      Other
      Phenotype Manifest In
      Enhanced by
      Enhancer of
      Statement
      Reference

      Nrg180.I.UAS/beat-IaUAS.cFa, Scer\GAL4eg-Mz360 is an enhancer of larval EW neuron phenotype of Scer\GAL4eg-Mz360, unc-5UAS.Tag:HA,Tag:SS(wg)

      (Zarin et al., 2014)

      Nrg180.I.UAS, beat-IaUAS.cFa, Fas2UAS.cLa, Scer\GAL4eg-Mz360 is an enhancer of larval EW neuron phenotype of Scer\GAL4eg-Mz360, unc-5UAS.Tag:HA,Tag:SS(wg)

      (Zarin et al., 2014)

      Nrg180.I.UAS/Df(2R)eve, Scer\GAL4eve.RN2 is an enhancer of larval VUM motor neuron phenotype of Scer\GAL4eve.RN2, beat-IaUAS.cFa

      (Zarin et al., 2014)

      Nrg180.I.UAS/Scer\GAL4eve.RN2 is an enhancer of larval VUM motor neuron phenotype of eveΔRP2A

      (Zarin et al., 2014)

      Nrg180.I.UAS, Scer\GAL4eve.RN2 is an enhancer of larval VUM motor neuron phenotype of Df(2R)eve, Scer\GAL4eve.RN2, unc-5UAS.Tag:HA,Tag:SS(wg)

      (Zarin et al., 2014)

      unc-5UAS.Tag:HA,Tag:SS(wg), Nrg180.I.UAS, Df(2R)eve, Scer\GAL4eve.RN2 is an enhancer of larval VUM motor neuron phenotype of Scer\GAL4eve.RN2, beat-IaUAS.cFa

      (Zarin et al., 2014)

      Nrg180.I.UAS, Scer\GAL4ppk.PU is an enhancer of larval dorsal multidendritic neuron ddaC | P-stage phenotype of Rab5S43N.UAS, Scer\GAL4ppk.PU

      (Zhang et al., 2014)

      Nrg180.I.UAS, Scer\GAL4ppk.PU is an enhancer of dendritic tree | P-stage phenotype of Rab5S43N.UAS, Scer\GAL4ppk.PU

      (Zhang et al., 2014)

      Nrg180.I.UAS, Scer\GAL4ppk.PU is an enhancer of dendritic tree | P-stage | somatic clone phenotype of Scer\GAL4ppk.PU, Vps4DN.UAS.Tag:HA

      (Zhang et al., 2014)

      Nrg180.I.UAS, Scer\GAL4ppk.PU is an enhancer of larval dorsal multidendritic neuron ddaC | P-stage | somatic clone phenotype of Scer\GAL4ppk.PU, Vps4DN.UAS.Tag:HA

      (Zhang et al., 2014)

      Nrg180.I.UAS, Scer\GAL4GMR.PF is an enhancer of cone cell phenotype of Scer\GAL4GMR.PF, edUAS.cBa

      (Islam et al., 2003)

      Nrg180.I.UAS, Scer\GAL4GMR.PF is an enhancer of eye photoreceptor cell phenotype of Scer\GAL4GMR.PF, edUAS.cBa

      (Islam et al., 2003)

      Nrg180.I.UAS, Scer\GAL4GMR.PF is an enhancer of eye phenotype of Scer\GAL4GMR.PF, edUAS.cBa

      (Islam et al., 2003)
      Suppressor of
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of Nrg180.I.Scer\UAS driven by Scer\GAL4elav.PU significantly partially suppresses the intersegmental nerve branch ISNb motor axon innervation defects (of m6/7) seen in mir-8Δ/mir-8Δ embryos. Expression of Nrg180.I.Scer\UAS driven by Scer\GAL4how-24B significantly partially (only 10%) suppresses the intersegmental nerve branch ISNb motor axon innervation defects (of m6/7) seen in mir-8Δ/mir-8Δ embryos.

      (Lu et al., 2014)

      Expression of Nrg180.I.Scer\UAS in an eveΔRP2A mutant background results in the exit of a single motoneuron of the pair in each hemisegment.

      Co-expression of beat-IaScer\UAS.cFa and Nrg180.I.Scer\UAS in a Df(2R)eve mutant background, under the control of Scer\GAL4eve.RN2 leads to a more robust CNS exit for motor neurons, with many hemisegments displaying exit of both motor neurons. There are two types of exit: unfasciculated, both axons from the same hemisegment chose a different nerve root, or fasciculated, where axons join and exit through the intersegmental nerve root.

      Co-expression of unc-5Scer\UAS.T:Ivir\HA1,T:SS-wg and Nrg180.I.Scer\UAS in a Df(2R)eve mutant background, under the control of Scer\GAL4eve.RN2 leads to a more robust CNS exit for motor neurons, with many hemisegments displaying exit of both motor neurons. There are two types of exit: unfasciculated, both axons from the same hemisegment chose a different nerve root, or fasciculated, where axons join and exit through the intersegmental nerve root.

      Co-expression of unc-5Scer\UAS.T:Ivir\HA1,T:SS-wg, beat-IaScer\UAS.cFa and Nrg180.I.Scer\UAS in a Df(2R)eve mutant background, under the control of Scer\GAL4eve.RN2 leads to a more robust CNS exit for motor neurons, with many hemisegments displaying exit of both motor neurons. The number of hemisegments with one neuronal exit is reduced, compared to controls and single and double mutants.

      Combinatorial misexpression of unc-5Scer\UAS.T:Ivir\HA1,T:SS-wg with Nrg180.I.Scer\UAS under the control of Scer\GAL4eg-Mz360, results in lateral exit of 8% of EW axons.

      Triple misexpression of unc-5Scer\UAS.T:Ivir\HA1,T:SS-wg, beat-IaScer\UAS.cFa, and Nrg180.I.Scer\UAS in EW neurons, under the control of Scer\GAL4eg-Mz360, leads to lateral redirection of axons in 20% of hemisegments.

      Quadrupal misexpression of unc-5Scer\UAS.T:Ivir\HA1,T:SS-wg, beat-IaScer\UAS.cFa, Nrg180.I.Scer\UAS and Fas2Scer\UAS.cLa in EW neurons, under the control of Scer\GAL4eg-Mz360, leads to lateral redirection of axons in 33% of hemisegments.

      (Zarin et al., 2014)

      The addition of Nrg180.I.Scer\UAS enhances the rough eye phenotype seen in edScer\UAS.cBa, Scer\GAL4GMR.PF animals with a reduction in the number of ommatidia and a decrease in the number of bristles. In addition, a significantly higher percentage of ommatidia contain fewer photoreceptor and cone cells.

      (Islam et al., 2003)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      Nrg180.I.UAS/Scer\GAL4c316 partially rescues Nrgibx

      (Carhan et al., 2005)

      Nrg180.I.UAS/Scer\GAL4Mef2.247 partially rescues Nrgibx

      (Carhan et al., 2005)

      Nrg180.I.UAS/Scer\GAL430Y partially rescues Nrgibx

      (Carhan et al., 2005)
      Fails to rescue

      Nrg180.I.UAS/Scer\GAL4Ilp2.PR fails to rescue Nrg849

      (Clements et al., 2021)

      Nrg180.I.UAS/Scer\GAL4Tab2-201Y fails to rescue Nrgibx

      (Carhan et al., 2005)
      Comments

      Expression of Nrg180.I.Scer\UAS under the control of either Scer\GAL4Mef2.247, Scer\GAL430Y, Scer\GAL4Tab2-201Y or Scer\GAL4c316 does not result in any clear evidence of rescue the Nrgibx central brain phenotype.

      Expression of Nrg180.I.Scer\UAS under the control of either Scer\GAL4Mef2.247, Scer\GAL430Y or Scer\GAL4c316 results in a small but significant increase in the sexual receptivity of Nrgibx females, while expression under the control of Scer\GAL4Tab2-201Y has no significant effect.

      (Carhan et al., 2005)
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Nrg180.I.Scer\UAS
      Nrg180.I.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (12)