FlyBase Allele Report: Dmel\ed[UAS.cBa]

General Information

Symbol

Dmel\ed^UAS.cBa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Bai

FlyBase ID

FBal0121789

Feature type

allele

Associated gene

Dmel\ed

Associated Insertion(s)

Carried in Construct

P{UAS-ed.B}

Also Known As

UAS-ed

Key Links

Transgenic product class

wild_type

(Bai et al., 2001)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Bai et al., 2001)

Mutagen

in vitro construct

(Bai et al., 2001)

Progenitor genotype

Carried in construct

P{UAS-ed.B}

Cytology

Description

Expression of ed ORF is governed by UASt regulatory sequences.

(Bai et al., 2001)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^ap-md544

(Escudero et al., 2003)

visible, with Scer\GAL4^Bx-MS1096

(Escudero et al., 2003)

visible, with Scer\GAL4^C-765

(Escudero et al., 2003)

visible, with Scer\GAL4^GMR.PF

(Bai et al., 2001)

visible, with Scer\GAL4^sca-537.4

(Escudero et al., 2003)

visible, with Scer\GAL4^sca-C253

(Escudero et al., 2003)

Phenotype Manifest In

cone cell, with Scer\GAL4^GMR.PF

(Islam et al., 2003)

dorsocentral bristle, with Scer\GAL4^sca-537.4

(Escudero et al., 2003)

eye, with Scer\GAL4^GMR.PF

(Fetting et al., 2009, Islam et al., 2003, Bai et al., 2001)

eye photoreceptor cell, with Scer\GAL4^GMR.PF

(Islam et al., 2003, Bai et al., 2001)

eye photoreceptor cell, with Scer\GAL4^ro.PO

(Fetting et al., 2009)

lateral transverse muscle cell, with Scer\GAL4^twi.PG

(Swan et al., 2006)

macrochaeta, with Scer\GAL4^ap-md544

(Escudero et al., 2003)

macrochaeta, with Scer\GAL4^sca-537.4

(Rawlins et al., 2003)

macrochaeta, with Scer\GAL4^sca-C253

(Escudero et al., 2003)

macrochaeta | ectopic, with Scer\GAL4^ap-md544

(Escudero et al., 2003)

macrochaeta | ectopic, with Scer\GAL4^Bx-MS1096

(Escudero et al., 2003)

macrochaeta | ectopic, with Scer\GAL4^C-765

(Escudero et al., 2003)

macrochaeta | ectopic, with Scer\GAL4^sca-537.4

(Escudero et al., 2003)

microchaeta, with Scer\GAL4^sca-537.4

(Escudero et al., 2003)

ommatidial precursor cluster, with Scer\GAL4^hs.2sev

(Fetting et al., 2009)

ommatidium, with Scer\GAL4^hs.2sev

(Fetting et al., 2009)

ommatidium, with Scer\GAL4^ro.PO

(Fetting et al., 2009)

scutellar bristle, with Scer\GAL4^sca-537.4

(Escudero et al., 2003)

sensory mother cell, with Scer\GAL4^sca-537.4

(Rawlins et al., 2003)

wing, with Scer\GAL4^Bx-MS1096

(Bai et al., 2001)

wing vein, with Scer\GAL4^Bx-MS1096

(Bai et al., 2001)

Detailed Description

Statement

Reference

Ommatidia often under-rotate in animals expressing ed^Scer\UAS.cBa under the control of Scer\GAL4^hs.2sev.

Animals expressing ed^Scer\UAS.cBa under the control of Scer\GAL4^ro.PF show mostly normal rotation of the ommatidia. Some ommatidia are missing photoreceptors.

Expression of ed^Scer\UAS.cBa under the control of Scer\GAL4^GMR.PF results in severe disruption of adult eye tissue.

(Fetting et al., 2009)

Expression of ed^Scer\UAS.cBa under the control of Scer\GAL4^twi.PG results in mild defects in lateral transverse muscle morphology.

(Swan et al., 2006)

The number of macrochaetae/heminotum in animals expressing ed^Scer\UAS.cBa under the control of Scer\GAL4^sca-C253 is - anterior+posterior notopleural: 1.23 (some flies lack macrochaetae at this position, others have duplications at this position), presutural: 0.90, anterior supraalar: 0.95, posterior supraalar: 0.88, anterior postalar: 0.98, posterior postalar: 1.00, anterior+posterior dorsocentral: 1.58 and anterior+posterior scutellar: 0.58. This represents a mild suppression of notum macrochaetae and the appearance of some extra macrochaetae in the notopleural position. The number of macrochaetae/heminotum in animals expressing ed^Scer\UAS.cBa under the control of Scer\GAL4^C-765 is - anterior+posterior notopleural: 2.25, presutural: 1.25, anterior supraalar: 1.15, posterior supraalar: 1.03, anterior postalar: 1.20, posterior postalar: 1.03, anterior+posterior dorsocentral: 2.85 and anterior+posterior scutellar: 3.70. Expression of ed^Scer\UAS.cBa under the control of Scer\GAL4^sca-537.4 results in the partial suppression of some macrochaetae (such as dorsocentral and scutellar) and some microchaetae, and the occasional duplication of some macrochaetae on the notum. Expression of ed^Scer\UAS.cBa under the control of Scer\GAL4^ap-md544 at 20^oC results in the loss of macrochaetae at some positions and extra bristles at others on the notum. Expression of ed^Scer\UAS.cBa under the control of Scer\GAL4^Bx-MS1096 can result in extra macrochaetae on the notum.

(Escudero et al., 2003)

When ed^Scer\UAS.cBa is driven by Scer\GAL4^GMR.PF, animals exhibit a rough eye phenotype and loss of photoreceptor and cone cells. On average 10-15% of ommatidia miss photoreceptor or cone cells.

(Islam et al., 2003)

ed^Scer\UAS.cBa; Scer\GAL4^sca-537.4 flies have many bristles missing. Examination of larval wing discs at puparium formation reveals that very few sensillum precursor cells have been selected.

(Rawlins et al., 2003)

When expression of one copy of ed^Scer\UAS.cBa is driven by Scer\GAL4^GMR.PF, eyes are small and rough with reduced number of photoreceptors. When expression of two copies of ed^Scer\UAS.cBa is driven by Scer\GAL4^GMR.PF, eyes fail to develop at all. When expression is driven by Scer\GAL4^Bx-MS1096 wings are reduced in size and lack wing veins. When expression is driven by Scer\GAL4^T155 or Scer\GAL4^CY2 resulting eggs have completely normal dorsal appendages.

(Bai et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

ed^UAS.cBa/Scer\GAL4^GMR.PF is an enhancer of visible phenotype of Scer\GAL4^GMR.PF, kek1^UAS.cGa

(Bai et al., 2001)

ed^UAS.cBa/Scer\GAL4^GMR.PF is an enhancer of visible phenotype of Scer\GAL4^GMR.PF, sty^UAS.cHa

(Bai et al., 2001)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^twi.PG, ed^UAS.cBa has lateral transverse muscle cell phenotype, enhanceable by Grip^ex36

(Swan et al., 2006)

Scer\GAL4^GMR.PF, ed^UAS.cBa has cone cell phenotype, enhanceable by Fas1::Nrg^GPI.UAS, Scer\GAL4^GMR.PF

(Islam et al., 2003)

Scer\GAL4^GMR.PF, ed^UAS.cBa has eye phenotype, enhanceable by Fas1::Nrg^GPI.UAS, Scer\GAL4^GMR.PF

(Islam et al., 2003)

Scer\GAL4^GMR.PF, ed^UAS.cBa has eye photoreceptor cell phenotype, enhanceable by Fas1::Nrg^GPI.UAS, Scer\GAL4^GMR.PF

(Islam et al., 2003)

Scer\GAL4^GMR.PF, ed^UAS.cBa has cone cell phenotype, enhanceable by Nrg^167.UAS, Scer\GAL4^GMR.PF

(Islam et al., 2003)

Scer\GAL4^GMR.PF, ed^UAS.cBa has eye phenotype, enhanceable by Nrg^167.UAS, Scer\GAL4^GMR.PF

(Islam et al., 2003)

Scer\GAL4^GMR.PF, ed^UAS.cBa has eye photoreceptor cell phenotype, enhanceable by Nrg^167.UAS, Scer\GAL4^GMR.PF

(Islam et al., 2003)

Scer\GAL4^GMR.PF, ed^UAS.cBa has cone cell phenotype, enhanceable by Nrg^180.I.UAS, Scer\GAL4^GMR.PF

(Islam et al., 2003)

Scer\GAL4^GMR.PF, ed^UAS.cBa has eye photoreceptor cell phenotype, enhanceable by Nrg^180.I.UAS, Scer\GAL4^GMR.PF

(Islam et al., 2003)

Scer\GAL4^GMR.PF, ed^UAS.cBa has eye phenotype, enhanceable by Nrg^180.I.UAS, Scer\GAL4^GMR.PF

(Islam et al., 2003)

NOT Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, ed^UAS.cBa has eye photoreceptor cell phenotype, non-enhanceable by Nrg^l4, Scer\GAL4^GMR.PF

(Islam et al., 2003)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, ed^UAS.cBa has cone cell phenotype, suppressible by Nrg^l4, Scer\GAL4^GMR.PF

(Islam et al., 2003)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, ed^UAS.cBa has eye photoreceptor cell phenotype, non-suppressible by Nrg^l4, Scer\GAL4^GMR.PF

(Islam et al., 2003)

Enhancer of

Statement

Reference

ed^UAS.cBa/Scer\GAL4^twi.PG is an enhancer of ventral longitudinal muscle cell phenotype of Grip^ex36

(Swan et al., 2006)

ed^UAS.cBa/Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, kek1^UAS.cGa

(Bai et al., 2001)

ed^UAS.cBa/Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, sty^UAS.cHa

(Bai et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of ed^Scer\UAS.cBa, under the control of Scer\GAL4^twi.PG, in a Grip^ex36 background, greatly enhances the muscle defects of Grip^ex36 embryos; ventrolateral muscles are more severely deranged and often appear to adhere to other muscles, whereas lateral transverse muscles split.

(Swan et al., 2006)

The addition of Nrg^{180.I.Scer\UAS} enhances the rough eye phenotype seen in ed^Scer\UAS.cBa, Scer\GAL4^GMR.PF animals with a reduction in the number of ommatidia and a decrease in the number of bristles. In addition, a significantly higher percentage of ommatidia contain fewer photoreceptor and cone cells. The addition of Nrg^l4/+ significantly suppresses the loss of cone cell phenotype seen in ed^Scer\UAS.cBa, Scer\GAL4^GMR.PF animals but has no effect on photoreceptor loss. The addition of Fas1::Nrg^GPI.Scer\UAS enhances the rough eye phenotype seen in ed^Scer\UAS.cBa, Scer\GAL4^GMR.PF.

(Islam et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

ed^UAS.cBa/Scer\GAL4^hh-Gal4 rescues ed^GD79

(Yue et al., 2012)

ed^UAS.cBa/Scer\GAL4^C-765 rescues ed^1X5

(Chang et al., 2011)

ed^UAS.cBa/Scer\GAL4^C-765 rescues ed^1X5

(Escudero et al., 2003)

Comments

Expression of ed^Scer\UAS.cBa rescues the posterior wing overgrowth phenotype seen when ed^GD79 is expressed under the control of Scer\GAL4^hh-Gal4.

(Yue et al., 2012)

Ubiquitous expression of ed^Scer\UAS.cBa under the control of Scer\GAL4^C-765 rescues the cell sorting phenotypes seen in ed^1X5 mutant wing disc clones. The boundary between the clone and the surrounding wild type cells is no longer smooth edged, instead appearing jagged as in wild type. Normal levels of shg are seen.

(Chang et al., 2011)

The poor viability of ed^1X5 homozygous clones in the wing disc is rescued if the clones are induced in a background where ed^Scer\UAS.cBa is expressed under the control of Scer\GAL4^C-765.

(Escudero et al., 2003)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

ed^Scer\UAS.cBa

ed^UAS.cBa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Bai

(Bai et al., 2001)

Secondary FlyBase IDs

References (10)

Report Sections

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