FB2024_03 , released June 25, 2024
Allele: Dmel\eveΔRP2A
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General Information
Symbol
Dmel\eveΔRP2A
Species
D. melanogaster
Name
FlyBase ID
FBal0155322
Feature type
allele
Associated gene
Dmel\eve
Associated Insertion(s)
Carried in Construct
P{eve.ΔRP2A}
Also Known As
eveΔRP2, RP2A
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Fujioka et al., 2003)
Progenitor genotype
eveE+L.9.2
(Fujioka et al., 2003)
Carried in construct
P{eve.ΔRP2A}
Cytology
Description

Sequence from +8.2kb to +9.2kb (coordinates relative to the eve transcription start site) of the eve genomic region has been deleted, thus sequences from -6.4kb to +8.2kb of eve are present.

(Fujioka et al., 2003)
Allele components
Component
Use(s)
Regulatory region(s)
eve
eve_-6.4/+8.2
Encoded product / tool
eve
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      aCC and RP2 fail to exit the CNS in eveΔRP2A mutants.

      (Zarin et al., 2014)

      eveΔRP2A mutants exhibit a failure of aCC/RP2 motoneurons to project out of the ventral nerve cord. In aCC, these effects are highly penetrant and observed at several stages, whereas in RP2 the effects are partially penetrant at stage 12 and almost absent at stage 15.

      (Garces and Thor, 2006)

      Homozygous Df(2R)eve embryos carrying two copies of eveΔRP2A show defects in the RP2, aCC and pCC neurons.

      Homozygous Df(2R)eve embryos carrying both eveΔRP2A and eveΔRP2B show defects in the RP2, aCC and pCC neurons.

      (Fujioka et al., 2003)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      NOT suppressed by
      Statement
      Reference

      eveΔRP2A has abnormal neuroanatomy phenotype, non-suppressible by Scer\GAL4eve.RN2/grnUAS.cBa

      (Garces and Thor, 2006)
      Enhancer of
      Statement
      Reference

      eveΔRP2A is an enhancer of abnormal neuroanatomy phenotype of Scer\GAL4eg-Mz360, unc-5UAS.Tag:HA,Tag:SS(wg)

      (Zarin et al., 2014)

      eveΔRP2A, beat-Ia[+], eve[+], beat-Ia3 is an enhancer of abnormal neuroanatomy phenotype of unc-58

      (Zarin et al., 2014)

      eveΔRP2A/eve[+] is an enhancer of abnormal neuroanatomy phenotype of unc-58

      (Zarin et al., 2014)

      eveΔRP2A is an enhancer of abnormal neuroanatomy phenotype of Nrgl7

      (Zarin et al., 2014)
      Other
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      A transheterozygous combination of eveΔRP2A/+, unc-58/+ reduces beat-Ia levels to 50% and significantly increases the defects seen in intersegmental nerves, with the number of axons exhibiting stalling increasing from 3% to 7.5% in eveΔRP2A/+, unc-58/+ transheterozygotes and eveΔRP2A/+, unc-58/+, beat-Ia3/+ triple heterozygotes, respectively.

      Expression of unc-5Scer\UAS.T:Ivir\HA1,T:SS-wg in an eveΔRP2A mutant background results in 11% of hemisegments exhibiting dual motoneuron exit. These mutants exhibit an increase in dorsal projections in 67% of hemisegments.

      Expression of beat-IaScer\UAS.cFa in an eveΔRP2A mutant background results in the exit of a single motoneuron of the pair in each hemisegment. These mutants exhibit an increase in dorsal projections in 36% of hemisegments.

      Expression of Fas2Scer\UAS.cLa in an eveΔRP2A mutant background results in the exit of a single motoneuron of the pair in each hemisegment. These mutants exhibit an increase in dorsal projections in 25% of hemisegments.

      Expression of Nrg180.I.Scer\UAS in an eveΔRP2A mutant background results in the exit of a single motoneuron of the pair in each hemisegment.

      Removing one copy of eve in Nrgl7/Y mutants results in more severe intersegmental nerve guidance defects with a significant increase in axon stalling upon the addition of eveΔRP2A.

      (Zarin et al., 2014)

      eveΔRP2A, Df(2R)eve/+ ; unc-58/+ double heterozygous embryos show guidance defects in the intersegmental nerve.

      (Zarin et al., 2012)

      Expression of grnScer\UAS.cBa in the aCC/RP2 neurons, under the control of Scer\GAL4eve.RN2 does not rescue the failure of aCC to project its axon out of the ventral nerve cord in eveΔRP2A mutants.

      (Garces and Thor, 2006)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      eveΔRP2A partially rescues eveunspecified

      (Fujioka et al., 2003)
      Comments

      Two copies of eveΔRP2A fully rescue segmentation function in eve null mutants. However, at the extended germband stage, eve expression in the mesoderm is often weak or missing.

      Homozygous Df(2R)eve embryos carrying two copies of eveΔRP2A show defects in the RP2, aCC and pCC neurons.

      eve null mutants carrying a copy of eveΔRP2A and eveΔRP2C in trans show normal mesodermal expression of eve and normal segmentation and are efficiently rescued to adulthood.

      (Fujioka et al., 2003)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      Df(2R)eveΔRP2A
      (Lacin et al., 2009)
      RP2A
      (Zarin et al., 2012)
      eveΔRP2
      (Zarin et al., 2012)
      eveΔRP2A
      Name Synonyms
      Secondary FlyBase IDs
        References (6)