FB2024_03 , released June 25, 2024
Allele: Dmel\grnUAS.cBa
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General Information
Symbol
Dmel\grnUAS.cBa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Brown
FlyBase ID
FBal0120484
Feature type
allele
Associated gene
Dmel\grn
Associated Insertion(s)
Carried in Construct
P{UAS-grn.B}
Also Known As
UAS-grn
Key Links
Transgenic product class
cDNA
(Brown and Castelli-Gair Hombria, 2000)
wild_type
(Brown and Castelli-Gair Hombria, 2000)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Brown and Castelli-Gair Hombria, 2000)
wild_type
(Brown and Castelli-Gair Hombria, 2000)
Mutagen
in vitro construct
(Brown and Castelli-Gair Hombria, 2000)
Progenitor genotype
Carried in construct
P{UAS-grn.B}
Cytology
Description

UASt regulatory sequences drive expression of a grn cDNA which includes 5' UTR and complete coding sequences.

(Brown and Castelli-Gair Hombria, 2000)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
grn
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of grnScer\UAS.cBa in dMP2 neurons, under the control of Scer\GAL4Vap.P0201 has no effect on dMP2 pathfinding.

      (Garces and Thor, 2006)

      In most larvae expressing grnScer\UAS.cBa under the control of Scer\GAL4h-1J3, the denticle belts of the alternating segments are reduced or missing, leaving a naked cuticle area. The head skeleton is abnormal in 9-32% of embryos and tail invagination is seen in 6-20%, depending on the number of copies of grnScer\UAS.cBa and the temperature of culture. In embryos with invaginated tails, the spiracles are now at the end of a long tubular structure formed by segments posterior to A8 that normally would be on the outside of the embryo. This structure includes cells from A8, A9, A10 and the telson.

      (Brown and Castelli-Gair Hombria, 2000)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhancer of
      NOT Suppressor of
      Statement
      Reference

      Scer\GAL4eve.RN2/grnUAS.cBa is a non-suppressor of abnormal neuroanatomy phenotype of eveΔRP2A

      (Garces and Thor, 2006)
      Phenotype Manifest In
      Enhancer of
      NOT Suppressor of
      Statement
      Reference

      Scer\GAL4eve.RN2/grnUAS.cBa is a non-suppressor of larval DA1 motor neuron phenotype of eveΔRP2A

      (Garces and Thor, 2006)
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of grnScer\UAS.cBa in the aCC/RP2 neurons, under the control of Scer\GAL4eve.RN2 does not rescue the failure of aCC to project its axon out of the ventral nerve cord in eveΔRP2A mutants.

      Expression of grnScer\UAS.cBa and eveScer\UAS.cBa in dMP2 neurons, under the control of Scer\GAL4Vap.P0201 enhances the eveScer\UAS.cBa dMP2 pathfinding defect, resulting in a high frequency of lateral exit out from the ventral nerve cord (in approximately 45% of cases).

      (Garces and Thor, 2006)

      Simultaneous expression of upd1Scer\UAS.cZa, emsScer\UAS.cSa, ctScer\UAS.cLa and grnScer\UAS.cBa, under the control of Scer\GAL469B allows formation of spiracle-like tubular structures in Abd-BM1/Abd-BM1 embryos.

      (Lovegrove et al., 2006)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL4eve.RN2/grnUAS.cBa rescues grn7L/Df(3R)dsx3

      (Garces and Thor, 2006)

      Scer\GAL4eve.RN2/grnUAS.cBa rescues Df(3R)dsx3/grnSPJ9

      (Garces and Thor, 2006)
      Partially rescues

      grnUAS.cBa/Scer\GAL4eve.CQ2 partially rescues grn7L/Df(3R)dsx3

      (Garces and Thor, 2006)

      grnUAS.cBa/Scer\GAL4eve.CQ2 partially rescues Df(3R)dsx3/grnSPJ9

      (Garces and Thor, 2006)

      Scer\GAL4salm-459.2/grnUAS.cBa partially rescues grn7J

      (Brown and Castelli-Gair Hombria, 2000)
      Comments

      Expression of grnScer\UAS.cBa in the aCC/RP2 neurons, under the control of Scer\GAL4eve.RN2 efficiently rescues grn7L/Df(3R)dsx3 and grnSPJ9/Df(3R)dsx3 mutant axon pathfinding (with 100% muscle 1/9 innervation). By contrast, expression of grnScer\UAS.cBa in the U motoneurons, under the control of Scer\GAL4eve.CQ2 only partially rescues the grn7L/Df(3R)dsx3 and grnSPJ9/Df(3R)dsx3 mutant phenotypes, with approximately 54% of muscles 1/9 being innervated.

      (Garces and Thor, 2006)

      Partially rescues the posterior spiracle phenotype of grn7J embryos when expressed under the control of Scer\GAL4salm-459.2.

      (Brown and Castelli-Gair Hombria, 2000)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      grnScer\UAS.cBa
      grnUAS.cBa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Brown
      (Brown and Castelli-Gair Hombria, 2000)
      Secondary FlyBase IDs
        References (5)