FlyBase Allele Report: Dmel\ct[UAS.cLa]

General Information

Symbol

Dmel\ct^UAS.cLa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Ludlow

FlyBase ID

FBal0051681

Feature type

allele

Associated gene

Dmel\ct

Associated Insertion(s)

Carried in Construct

P{UAS-ct.L}

Also Known As

UAS-cut, UAS-ct

Key Links

Transgenic product class

wild_type

(Ludlow et al., 1996)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Ludlow et al., 1996)

Mutagen

in vitro construct

(Ludlow et al., 1996)

Progenitor genotype

Carried in construct

P{UAS-ct.L}

Cytology

Description

UASt regulatory sequences drive expression of the full-length ct coding sequence.

(Ludlow et al., 1996)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^dpp.blk1

(Johnston et al., 1998)

Phenotype Manifest In

abdominal 1 lateral pentascolopidial chordotonal organ lch5, with Scer\GAL4^h-1J3

(Ludlow et al., 1996)

abdominal 2 lateral pentascolopidial chordotonal organ lch5, with Scer\GAL4^h-1J3

(Ludlow et al., 1996)

abdominal 3 lateral pentascolopidial chordotonal organ lch5, with Scer\GAL4^h-1J3

(Ludlow et al., 1996)

abdominal 4 lateral pentascolopidial chordotonal organ lch5, with Scer\GAL4^h-1J3

(Ludlow et al., 1996)

abdominal 5 lateral pentascolopidial chordotonal organ lch5, with Scer\GAL4^h-1J3

(Ludlow et al., 1996)

abdominal 6 lateral pentascolopidial chordotonal organ lch5, with Scer\GAL4^h-1J3

(Ludlow et al., 1996)

abdominal 7 lateral pentascolopidial chordotonal organ lch5, with Scer\GAL4^h-1J3

(Ludlow et al., 1996)

adepithelial cell of wing disc, with Scer\GAL4¹¹⁵¹

(Sudarsan et al., 2001)

antenna, with Scer\GAL4^dpp.blk1

(Johnston et al., 1998)

arista, with Scer\GAL4^dpp.blk1

(Johnston et al., 1998)

indirect flight muscle cell, with Scer\GAL4¹¹⁵¹

(Sudarsan et al., 2001)

leg | ectopic, with Scer\GAL4^dpp.blk1

(Johnston et al., 1998)

mechanosensory chaeta | increased number, with Scer\GAL4^sca-P309

(Jhaveri et al., 2000)

Detailed Description

Statement

Reference

ct^Scer\UAS.cLa; Scer\GAL4¹¹⁵¹ flies have almost complete loss of indirect flight muscles, whereas direct flight muscles are unaffacted (10/10 examined). In pupae of this genotype there is an approximately 10-fold reduction in the number of myoblasts (adepithelial cells) overlying the indirect flight muscle larval templates, and a greatly increased incidence of cell death in these cells.

(Sudarsan et al., 2001)

When ct^Scer\UAS.cLa is driven in proneural domains by Scer\GAL4^sca-P309, no affect is seen on the development of any of the olfactory sense organs. However ectopic large external sense organs resembling mechanosensory bristles are seen.

(Jhaveri et al., 2000)

When expression is driven by Scer\GAL4^dpp.blk1 flies are severely malformed due to loss of structures that normally arise in the dpp expression domain in the eyes, legs and wings. In 43% of flies antenna is transformed to leg. Structures such as claws appear at the distal end of, or in place of, the arista. This phenotype resembles that of dominant or ectopic expression Antp alleles.

(Johnston et al., 1998)

Scer\GAL4^C96 induced expression in ct⁶ at 24^oC causes some lethality. The survivors exhibit some rescue of the ct⁶ mutant phenotype: the wing margin, slender and stout mechanosensory bristles, non-innervated hairs. The number of chemosensory bristles is further reduced. Greater extent of rescue of wing marginal tissue and sensory bristles is observed at 17^oC.

(Ludlow et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Other

Statement

Reference

Abd-B^M1, Scer\GAL4^69B, ct^UAS.cLa, ems^UAS.cSa, grn^UAS.cBa, upd1^UAS.cZa has embryonic/larval spiracle | ectopic phenotype

(Lovegrove et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Simultaneous expression of upd1^Scer\UAS.cZa, ems^Scer\UAS.cSa, ct^Scer\UAS.cLa and grn^Scer\UAS.cBa, under the control of Scer\GAL4^69B allows formation of spiracle-like tubular structures in Abd-B^M1/Abd-B^M1 embryos.

(Lovegrove et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

UCut25-9

(Johnston et al., 1998)

ct^Scer\UAS.cLa

ct^UAS.cLa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Ludlow

(Ludlow et al., 1996)

Secondary FlyBase IDs

References (11)

Report Sections

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